The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3- 0.070169X1 2-0.292175X2 2-0.008818X3 2, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.
Cast fishing gear needs some time for fishing progress, and catches and their composition by submerged time can change by several cause. Therefore, it is very importance to study fishing capacity of fishing gear by submerged time. This study is to investigate catches and their composition of shrimp trap, that is used in the coastal of the East Sea, and to find the fittest lifting time of trap. Experimental term are September 2006 and August 2007, the location is the coastal of Oho, Goseong, Gangwondo, Korea and one hundred trap is used at each casting and lifting of gear. For convenience of description, survey of 2006 and 2007 are dented as experimental code 1 and 2, and submerged time 21hr, 43hr and 66hr are dented as code A, B and C. The result of obtained from the above approach are summarized as follows: Many Northern shrimps(Pandalus eous), dominated 96.36%, are only catched in experimental code A, and in code B and C, some of coonstripe shrimp(Pandalus hypsinotus) and few morotoge shrimp(Pandalopsis japonica) are catched. CPUE of code 1A, 1B and 1C per trap were 21.67g, 29.51g and 28.48g, and those of code 2A, 2B and 2C per trap were 25.44g, 32.93g and 33.36g. Therefore, 24.66% of catch increased according as submerged time passes from 1 day to 2 days, and almost no change of catch was to be -1.1%. Carapace length of code 1A, 1B and 1C were 23.77mm, 25.00mm and 25.57mm, and those of code 2A, 2B and 2C per trap were 23.83mm, 24.95mm and 25.45mm. Thus, the more submerged time is, the less catch of small fish is and the more catch of large fish is. Consequently, fit lifting time of shrimp trap is after 2 days, and if considered trouble of fishing gear and condition of catch, the fittest lifting time is the third successive day of casting date.