참외를 껍질째 먹기 위하여 착과 5일후부터 과실에 배, 사과, 포도봉지를 씌워 재배한 결과, 봉지내의 온도는 외기온에 비하여 주간에는 낮고 야간에는 높았으며 습도는 주간에는 높고 야간에는 낮았다. 무처리구에 비하여 봉지재배로 과장은 짧고 과폭은 좁고 과육두께는 얇고 과중은 가벼운 경향이었으나 처리간 차이는 없었다. 과육 및 태좌부의 당도는 봉지재배 처리구에서 낮은 경향이었으나 처리간 차이는 없었다. 그러나 과실의 당도는 봉지제거 직후 보다는 봉지제거 5일후 조사에서 높아지는 것을 알 수 있었다. 봉지재배로 과피의 경도 및 색도가 낮았다. 이와 같이 봉지재배로 과피의 경도가 낮아 껍질째 먹기는 쉬워졌으나 과피의 색도 및 당도가 낮아 금후 보완이 필요한 것으로 생각된다.
오미자 추출물의 가열 및 저장에 의한 변색을 지연시킴으로써 식품 소재로써 오미자 추출물의 이용도를 증진시키고자 식품에 가장 광범위하게 이용되는 첨가물인 당과 색소 안정화제의 효과를 조사하였다. 단당류로써 glucose, fructose, 이당류로써 sucrose, maltose를 처리한 결과 단당류는 가열에 의한 변색을 촉진시켰고 이당류는 가열에 의한 변색을 억제시키는 효과가 있어 maltose를 10%처리하였을 때 40%정도 변색이 억제되었다. 색소 안정화제 로 각각 1%와 5%의 maltodextrin, β, γ-cyclodextrin, ascorbic acid, phosphoric acid를 첨가하여 조사한 결과 β- 및 γ-cyclodextrin과 maltodextrin 5% 처리구의 경우 DI값이 대조군보다 낮게 나타나 가열 변색에 대한 안정화 효과를 나타내었다. 복잡한 구성성분으로 인하여 matrix 효과를 나타내는 식품에의 적용효과를 조사하고자 오미자 색소 추출물을 첨가한 겔 시스템을 모델 식품으로 사용하여 25℃ 및 35℃에서 저장하면서 표면 색도의 변화를 측정하였다. 저장 온도 및 기간이 증가할수록 a값이 감소하는 경향을 나타내었으며 maltodextrin을 5.0% 첨가한 경우 a 값이 15~20%증가하여 matrix effect가 고려된 실제 식품에서도 색소안정화 효과가 확인되었다.
Tomato fruit color, which is the most visible characteristic among the other fruit traits, is considered to have a substantial influence on consumers. The pink-colored tomatoes with high soluble solids content are considerably preferred especially in Asia compared to the other colors. Generally the pink fruit trait of tomatoes is easily determined by visual examination of intact fruit, however, it is technically determined by the characteristic of the fruit peel. The pink trait is regulated by variations of the SlMYB12(y) gene located on chromosome 1, which controls the accumulation of the naringenin chalcone, which comprises a large proportion of flavonoids. In this study, we developed a derived Cleaved Amplified Polymorphic Sequences (dCAPS) marker and a sequence characterized amplified regions (SCAR) marker in order to discriminate of pink/non-pinktomatoes in the domestic breeding lines. Quantitative RT-PCR analysis indicated that the SlMYB gene is highly expressed in non-pink fruit peel, whereas the expression is significantly lowered in the pink fruit peel. These gene based markers are expected to enhance the efficiency and accuracy of selection pink-tomatoes in tomato breeding programs.
Fresh market tomato cultivars are divided largely based on fruit color appearance (red or pink), which is attributed by the trait of peel. It had been reported that mutation of the Slmyb12 gene suppresses synthesis of yellow-colored flavonoid (naringenin chalcone) in peel and causes pinkish tomato fruit. Whereas wild-type tomato plants synthesizing naringenin chalcone produce yellow-colored peel, which resulting in the fruit appearance to be red. The present study was performed to investigate the association between the Slmyb12 and fruit color of domestic tomato inbreed lines. A SCAR marker was developed from an Indel mutation site (72bp insertion in exon3) of the Slmyb12, and tested on 22 and 18 red and pink-fruited inbred lines, respectively. Unexpectedly, the results showed that all inbred lines tested had wild-type Slmyb12. The full length sequences of the Slmyb12 were cloned from two inbred lines (FCR1 and FCP1), but the sequence alignment did not identify any nucleotide variations within this gene. Furthermore, scanning of SNPs between FCR1 and FCP1 using SolCAP Tomato SNP array) found no SNPs for Slmyb12. To delimit the genomic region of the gene conferring fruit color of domestic tomato lines, we are analyzing SNPs in the genes adjacent to the Slmyb12 using an F2 population derived from FCR1 x FCP1. So far, one SNP located at 1,750kbp downstream from the stop codon of Slmyb12 was mapped using 54 F2 plants and 83% of phenotype-marker association was revealed, demonstrating that the fruit color is controlled bySlmyb12 indeed, or other neighboring gene(s) involved in the pathway of naringenin chalcone synthesis. Further study with more SNPs will clear up this question.
흑색계(캠벨어얼리, 거봉, 머스캇베일리에이)와 적색계(노스레드)의 4종류의 포도 품종에 대하여 안토시아닌의 조성을 분석하고 과피에서 추출한 안토시아닌 색소의 색발현을 조사하였다. 조사한 4종류의 품종에서 모두 12종류의 안토시아닌을 확인할 수 있었으며 흑색계 품종에서 특히 머스캇베일리에이 품종은 안토시아닌 조성이 가장 다양한 반면 적색계의 노스레드는 안토시아닌 조성이 비교적 단순하였다. 포도 과피에서 안토시아닌 농축액을 조제한 후 품종별 색 발현
Apple fruit grading is largely dependant on skin color degree. This work reports about the possibility of nondestructive assessment of apple fruit color using infrared(NIR) reflectance spectroscopy. NIR spectra of apple fruit were collected in wavelength range of 1100~2500nm using an InfraAlyzer 500C(Bran+Luebbe). Calibration as calculated by the standard analysis procedures MLR(multiple linear regression) and stepwise, was performed by allowing the IDAS software to select the best regression equations using raw spectra of sample. Color degree of apple skin was expressed as 2 factors, anthocyanin content by purification and a-value by colorimeter. A total of 90 fruits was used for the calibration set(54) and prediction set(36). For determining a-value, the calibration model composed 6 wavelengths(2076, 2120, 2276, 2488, 2072 and 1492nm) provided the highest accuracy : correlation coefficient is 0.913 and standard error of prediction is 4.94. But, the accuracy of prediction result for anthocyanin content determining was rather low(R of 0.761).
The seasonal flower, leaf and fruit color characterlstics of deciduous woody landscape plants were investigated through the field survey in Suwon, Korea from January 1, 1992 toMarch 20, 1993.The results were as follows ;1. There were 12 cases in the combination of leaf spreading, anthesis, deblossom, fruit coloring,fruit falling, fall foliage coloring and leaf falling of deciduous woody landscape plants(163 spe-cies) investigated. And species of the leaf spreading, anthesis, deblossom, fall foliage coloringand leaf falling were 71(43.9%). And species of the leaf spreading, anthesis, deblossom,fruit coloring, fall foliage coloring, leaf falling and fruit falling were 40(24.5% ).2. The total continuing period of the flowering, fall foliage coloring and fruit coloring of decid-uous woody landscape plants(79 species) investigated varied from 2 months to more than 9months. And the species over 3 months below 4 months were 23(29.2%).3. There were 24 cases in the combination of color of flower, fall foliage and fruit of deciduouswoody landscape plants(79 species) investigated. And the species of the white flower, red fall fo-liage and red fruit were 16(20.3% ).