During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or 5 ℃) and supplemented them with 1mM glucose and 0.5mM glutamine as a source of carbohydrate metabolites. After recovery of COCs and in vitro fertilization, a significantly higher number of oocytes developed into blastocysts. The developmental competence of embryos that were originated from ovaries preserved at 15 ℃ was increased compared to those of 25 or 5 ℃. The maturation rate of oocytes was not differed between 24 and 36 h at 15 ℃ but showed lower than control group (71% versus 78%). In vitro-fertilized oocytes from ovaries stored at 25 ℃ for 24 h or at 5 ℃ for 24 h had a significantly decreased developmental potentials, but at 15 ℃ did not (27% versus 29% of blastocysts to develop into day 8). With these results, bovine ovaries can be preserved at 15 ℃ for 36 h without decreasing developmental capacity of in vitro-fertilized oocyte at least to the blastocyst stage. This information provides valuable information of preserving ovaries for embryo transfer or in vitro embryo production.

The PHBV nonofibrous membrane fabricated by electron-spinning method for tissue engineered bone regeneration scaffold was evaulated in terms of cellular prolieration and cryopreservation efficiency. The rat calvarial periosteum derived primary cells were cultured with PHBV nanofibrous membranes and analyzed the cellular proliferation and differention fashion and cryopreservation potential by in vitro MTT assay as well as ALP staining and Alizarine red staining with or without cryopreservation for 2 weeks. The rat calvarial periosteum derived primary cells cultured with PHBV nonofibrous membrane showed favorable proliferation and alkaline phosphatase activity with numerous mineral nodule formation regardless of cryopreservation, even though its efficiency was slightly decreased in cryopreserved condition. These findings suggest that PHBV nanofibrous membrane can be applicable as an efficient cell engineered membrane for guided bone regeneration or scaffold for tissue engineered bone regeneration.

본 연구는 항생제가 첨가되지 않은 돼지 혼합액상 정액을 17 정액 보관고에 보관하면서 보관일수의 증가가 따라 정자의 운동성, 정액 내 세균의 증식 여부 및 체외 수정란 생산 효율에 미치는 영향을 조사하고자 하였다. 정자의 운동성은 1일 (78.72.4%)에 비하여 3일(78.72.4%)과 5일째(64.82.4%)는 유의적으로(p<0.05) 낮은 운동성을 나타내었다. 보관일수에 따른 정액 내 세균수의 변화는 보관 5일이 Cfu로 0일과 3일의 Cfu와 C

Oxidative stress is one of the major causes of failure in in vitro storage of boar semen. Reactive oxygen species (ROS) are known to be important mediators of such stress. The present study examined the effects of pyruvate and taurine on sperm motility and expression of BAD, Cytochrome c, Caspase-3 and Cox-2 protein in in vitro storage of boar semen, and tested the effect of semen treated with antioxidant with or without hydrogen peroxide on the development of IVM/IVF porcine embryos. Semen samples were transported to the laboratory at 17℃ within 2 hr after collection and were treated with different concentration of pyruvate (1~10mM) and taurine (25~100mM) with or without 250uM H2O2 respectively. The supplementation of pyruvate and taurine increased sperm motility in boar semen during in vitro incubation at 37℃. Expression of apoptosis protein (BAD, cytochrome c, caspase-3 and cox-2) were reduced in the group of boar semen treated with pyruvate and taurine when compared to the other groups. The developmental rates of IVM/IVF porcine embryos fertilized by semen treated with pyruvate and taurine were significantly increased when compared to control (P<0.005). These results indicate that supplementation of pyruvate and taurine as antioxidants in boar semen extender can improve the semen quality and increase in vitro development of porcine IVM/IVF embryos when boar semen treated with antioxidants was used for in vitro fertilization.

본 연구는 고양이의 불임 해결을 위한 방안의 하나로써 체외수정란을 생산할 목적으로 난자의 형태, 보존 및 번식주기별 난자의 체외발생에 미치는 영향을 조사하였다. 1. 신선 난소로부터 회수한 난구세포부착 및 나화 난자를 각각 배양했을 때 GV 및 MI으로의 체외성숙율은 74.3%와 25.7%와 및 28.6%와 11.4%,로써 난구세포 부착 난자가 나화 난자의 비해 높은 체외성숙율을 나타냈다. 2. 휴지기, 난포기 및 황체기 단계로 구분하여 채취한 난구세포