본 연구는 유칼립투스(Eucalyptus pulverulenta) 잎에서 치료적 효과가 큰 α-pinene과 1,8-cineole 함량증가에 미치는 H2O2와 SA 처리의 영향에 대해 알아보기 위하여 수행하였다. 유칼립투스가지를 H2O2 수용액(0.3, 0.5, 1%)과 SA 수용액(0.1, 1mM)에 침지하거나 잎에 엽면살포(0.1mM SA, 1% H2O2)한 뒤 시간흐름(0, 0.5, 1, 2, 4시간)에 따른 α-pinene과 1,8-cineole의 함량변화를 분석한 결과, 침지처리에서 유칼립투스 잎의 α-pinene 함량(mg･L-1)은 0.1mM SA에 2시간 침지 후 1.62에서 5.48로 크게 증가하였으며(238.27%, p=0.012), 1,8-cineole 함량(mg･L-1)은 1mM SA에 4시간 침지 후 44.44에서 78.96으로 크게 증가하였다(77.66%, p=0.026). 살포처리에서는 0.1mM SA를 엽면살포 30분 후 α-pinene 함량(mg･L-1)은 1.62에서 3.91로(141.36%, p=0.007), 1,8-cineole 함량(mg･L-1)은 44.44에서 87.91로 증가하였다(97.82%, p=0.001). α-pinene과 1,8-cineole 모두 살포처리 30분 뒤 크게 증가하여, 엽면살포가 짧은 시간내에 방향화합물을 증가시키는데 침지처리보다 효과적이었으며, H2O2와 SA 처리는 α-pinene과 1,8-cineole의 함량증가에 유의한 영향이 있었다. 또한, SA의 처리가 H2O2보다 방향화합물 함량증가에 더 효과적이었으며, 유칼립투스 가지의 경우 원예치료 2시간 전에 0.1mM SA 용액에 담그거나 30분 전에 잎에 살포하면 monoterpene의 유칼립투스 함량이 크게 증가되었다.

Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ɑ-pinene on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ɑ-pinene, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ɑ-pinene in a dose-dependent manner. These results suggested that ɑ-pinene may be a useful agent for inhibiting the cariogenic properties of S. mutans.

α-Acaridial [2(E)-(4-methyl-3-pentenyl)butenedial] is a novel monoterpene secreted from the house dust mites. Because of its molecular nature of a highly reactive, small lipidic compound, we addressed whether α-acaridial might function as a haptenic allergen that induced allergic contact dermatitis. Mice sensitized with α-acaridial were challenged by the same antigen on the ear skin. After 2 days, significant ear swelling with a prominent infiltration of CD4+ T lymphocytes was observed. In vitro, α-acaridial exhibited an outstanding ability to quickly interact with and chemically modify a reference protein. Virtually all cysteine residues and a sizable fraction of lysine residues were found to be selectively modified, suggesting that α-acaridial could potentially interact with any proteins. Previously, numerous mite-derived proteinaceous allergens have been associated with contact dermatitis. Our study now emphasizes that small lipidic compounds released from mites comprise a new class of mite allergens, and therefore, is of significant medical implications.