In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 100 CFU/mL to 106 CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)- mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37oC. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 106 CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency.

본 논문에서는 반응표면법과 다목적최적화 기법을 이용한 유한요소모델개선기법의 절차를 제안하고 이를 저층의 철근콘 크리트건물의 모델개선에 적용하였다. 대상건물은 전단벽 신설 및 댐퍼부착을 위한 부재의 강재보강을 통해 내진보강이 이 루어진 건물로서 보강전후에 소형 가진기를 이용한 진동실험을 실시하여 동특성을 구하였다. 대상건물의 개선에 사용된 변 수는 기존콘크리트, 신규타설된 콘크리트, 조적의 탄성계수, 신축줄눈부의 스프링계수, 강재보강된 부재의 유효강성비이다. 보강전후 건물의 초기모델을 구축한 후 중심합성법에 따라 개선변수의 값을 변화시키면서 얻은 해석결과를 통해 고유진동 수의 오차와 모드형상의 오차를 나타내는 2개의 반응함수를 구하고, 이를 다목적최적화의 목적함수로 사용하였다

Multi-objective optimization using response surface methodology-based surrogate model was employed to find optimal design parameter of TMD installed on structure under the El Centro earthquake. It is found that the RSM based weighted multi-objective optimized damper improves frequency responses and root mean square displacements of the structure without TMD by 31.6% and 82.3% under El Centro earthquake, respectively, and has an equal or higher performance than the conventionally designed dampers with respect to frequency responses and root mean square displacements and when applied to earthquakes.