The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method’s limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.

The southern rough shrimp Trachysalambria curvirostris (Stimpson) was monthly sampled from the coastal area of Yeosu, Korea from June 2000 to May 2001 and its stomach contents were investigated. Mysids and amphipods were the most dominant prey, comprising

Ghost fishing으로 추정되는 유실된 플라스틱 붕장어 통발에 어획된 붕장어의 섭이 상태와 개체의 성장에 대해 조사하기 위하여, 통발 조업선에 어획된 붕장어와 트롤 그물에 어획된 붕장어를 이용하여 비교 분석하였다. 어획된 붕장어의 평균 전장은 연안 통발 조업선과 시험 조업에서 어획된 것이 33.6cm, 트롤 그물에 어획된 것이 48.9cm 그리고 수거된 유실 통발에 어획된 것이 45.6cm이었다. 이러한 체장의 분포는 붕장어가 성장하면서 연안에서 외양으로 나간다는 사실과 일치한다. 위내용물 조사에서 먹이를 섭이하지 못해 위내용물이 전혀 없었던 붕장어의 비율은 통발 조업선 5.0%, 트롤 4.2% 그리고 유실 통발 87.6%로 유실 통발에서 17～20배 높게 나타났다. 먹이로써 어류를 섭이한 붕장어의 비율은 통발 조업선이 98.7%, 트롤이 78.8% 그리고 유실 통발이 63.3%로 나타났으며, 그 외의 먹이 생물은 갑각류와 두족류 등이었다. 순체중과 전장을 이용하여 계산한 비만도는 트롤 어획물이 1.514이고, 유실 통발 어획물이 1.292로서 약 15%의 차이가 있었으며, 이들 자료에 대한 공 분산분석에서 실시한 t검정 결과 5% 유의수준에서 위치의 차이가 있는 것으로 나타났다.