Soybean [Glycine max (L.) Merr.] protein is a high quality source for food and feed. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein of mature soybean seed is a main antinutritional factor in soybean seed. The Le gene controls a lectin protein and Ti gene controls the KTI protein in soybean. Ti locus has been located on molecular linkage group A2 (chromosome 8) of soybean. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. Although this type is considered chlorophyll-deficient, it is fairly vigorous in growth. The objective of this research was to exam an agronomic traits of y9ti genotype selected from the breeding line. The seeds of y9ti genotype were planted in the field. Traits of maturity date, seed weight, and seed coat color were checked.

Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. Soybean Kunitz trypsin inhibitor protein has been proposed as one of the major antinutritional factor. The absence of Kunitz trypsin inhibitor protein in mature seed is inherited as a recessive allele designated ti. The objective of this research was to confirm independent inheritance between ti gene and y9 gene. The F1 seeds from Gaechuck#1 (Kunitz trypsin inhibitor protein absent, normal leaf type) x C-142 (Kunitz trypsin inhibitor protein present, leaf chlorophyll -deficient) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of Kunitz trypsin inhibitor protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.

Soybean [Glycine max (L.) Merr.] proteins are widely used for human and animal feed in the world. Glycinin (11S globulin) and β-conglycinin (7S globulin) account for storage protein from 70% to 80% in soybean seed. 7S globulin protein exhibits poorer nutritional and food processing properties. β-conglycinin is composed of α’, α, and β-subunits. α′-subunit of 7S globulin are main antinutritional factors in soybean seed. The absence of α′-subunit were controlled by single recessive alleles, cgy1. Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. The objective of this research was to confirm the linkage or independent assortment between cgy1 gene and y9 gene. The F1 seeds from C-142 (α′-subunit of 7S globulin present, leaf chlorophyll -deficient: Cgy1Cgy1y9y9 genotype) x PI line (α′-subunit of 7S globulin absent, normal leaf type: cgy1cgy1Y9Y9 genotype) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of α′-subunit of 7S globulin protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.

dlm mutants controlling disease lesion mimic leaf trait may be useful in basic research of disease hypersensitive response and programmed cell death in soybean. The study on genetic relationship between dlm trait and other morphological C trait, position of dlm allele on classical linkage group, and a molecular marker linked to dlm allele was little reported. Two populations [T173 (ffDlmDlm) x T363 (FFdlmdlm), T363 (dlmdlmY9Y9) x T135 (DlmDlmy9y9)] were made to find independent assortment or linkage between dlm locus and f locus or between dlm locus and y9 locus. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values suggested that the disease lesion mimic leaf, fasciation stem, and chlorophyll-deficient leaf traits were controlled by a single recessive gene. Segregation ratios of 78 Dlm_F_: 19 Dlm_ff: 17 dlmdlmF_ : 3 dlmdlmff based on F2 phenotype showed that dlm allele was inherited independently with the f allele controlling fasciation stem trait in soybean. Also, segregation ratios of from 149 Dlm_Y9_: 41 Dlm_y9y9: 38 dlmdlmY9_ : 5 dlmdlmy9y9 based on F2 phenotype confirmed that dlm allele was inherited independently with the y9 allele controlling chlorophyll- deficient leaf trait in soybean. From these results, dlm allele would not be located on linkage group 11 (molecular linkage group: D1b+W) and linkage group 14 (molecular linkage group: E) in soybean. This results will helpful to attempt to position the Dlm locus on the soybean molecular linkage map.

Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic stud-y9 locus. A mapping population con-sisting of 94 F2 progeny was derived from a cross between normal green leaf Clark (Y9 ) and chlorophy