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재배기간에 따른 쓴메밀(Fagopyrum tataricum Gaertner)싹의 항산화 활성 및 생리활성 평가 Changes of Antioxidant Activity as affected by cultivation period in Buckwheat (Fagopyrum species) Sprouts

김현영, 우소연, 서우덕, 이미자
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  • URLhttps://db.koreascholar.com/Article/Detail/404806
韓國食生活文化學會誌 (한국식생활문화학회지)
제35권 제6호 (2020.12)
pp.590-596
한국식생활문화학회 (The Korean Society Of Food Culture)
초록

Buckwheat (Fagopyrum species) has long been an excellent functional food. Besides, buckwheat sprouts contain various functional substances. In this study, we investigated the antioxidant activity of buckwheat sprouts in the context of cultivars harvested after different cultivation periods (0, 3, 5, 7, 9, 13, and 15 days after planting). Buckwheat sprouts were cultivated at 25oC for up to 15 days and then extracted with ethanol. Antioxidant components were then extracted from sprouts and leaves using a freeze dryer. The total polyphenolic content, flavonoid content, and antioxidant activity were then analyzed. The total polyphenol content increased from 32.26 mg GA eq/100 g for raw buckwheat to 114.75 mg GA eq/100 g after 7 days of cultivation. Also, the flavonoid content increased from 20.61 mg catechin eq/100 g (0 days) to 56.54 mg/g after 9 days of cultivation. The DPPH radical scavenging activity (concentration of extract at 0.25 mg/mL) increased from 7.89% at day 0 to 53.48% after 9 days of cultivation. Additionally, the ABTS radical scavenging activity increased from 10.26% at day 0 to 32.89% after 7 days of cultivation; of note, the activity decreased afterward. These results suggest that the best buckwheat sprouts with higher biological activities are those cultivated for 7-9 days. For a complete understanding of the potential of buckwheat sprouts as functional foods, we plan to further analyze their antioxidant activity in the future.

키워드
Buckwheatsproutantioxidant activitypolyphenolflavonoidchlorophyllfree amino acid
목차
Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 재료 및 시약
    2. 메밀싹 재배 및 methanol 추출물 제조
    3. 유리아미노산 및 GABA 분석
    4. 클로로필 a,b 함량 분석
    5. 총 폴리페놀 및 플라보노이드 함량
    6. 라디칼 소거능 측정
    7. 통계분석
III. 결과 및 고찰
    1. 메밀싹의 유리아미노산 및 GABA 함량 변화
    2. 메밀싹의 클로로필 a, b 함량
    3. 메밀싹의 재배 기간별 ABTS 및 DPPH 라디칼 소거능
    4. 메밀싹의 재배 기간별 총 폴리페놀 및 플라보노이드 함량
IV. 요약 및 결론
감사의 글
Conflict of Interest
References
저자
  • 김현영(농촌진흥청 국립식량과학원 작물기초기반과) | Hyun Young Kim (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) Corresponding author
  • 우소연(농촌진흥청 국립식량과학원 작물기초기반과) | So-Yeun Woo (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
  • 서우덕(농촌진흥청 국립식량과학원 작물기초기반과) | Woo Duck Seo (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
  • 이미자(농촌진흥청 국립식량과학원 작물기초기반과) | Mi Ja Lee (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)