Shading and soil environment are the main factors of growth and yield in ginseng (Panax ginseng C. A.Meyer). Ginseng yield is directly related to survival rate because of increased missing plant for their growing period. Underfield conditions, diseases and pests significantly affect plant survival rate. We evaluated the seedling establishment, growthand ginsenoside of the ginseng plants, under controlled management conditions in a plastic greenhouse, when their treatedwith different types of organic matter. Ginseng seeds were sown at a rate of three seeds per hole, and the seeding space mea-sured 10㎝×15㎝. Compared to the control, treatment of cattle manure vermicompost (CMV) was shown to increaseseedling establishment and decrease ginsenoside content. Root weights of plants treated with CMV were higher than those ofplants treated with other types of organic matter. In addition, seedling establishment of 2-year-old ginseng plants wasdecreased when it was compared to that of 1-year-old ginseng plants. Our results indicated that organic matter type and ratewere associated with seedling establishment, growth characteristic and ginsenoside content in greenhouse of ginseng direct-sowing culture.