To investigate skin-whitening effect of Adenophora triphylla var. japonica sprout extract, antioxidant activity, inhibitory effect on tyrosinase and melanin synthesis in B16/F10 melanoma cell were examined. Total phenolic content (246.25 ㎎ GAE/g) and total flavonoid content (303.94 ㎎ RE/g) of ethyl acetate fraction from Adenophora triphylla sprout (EFAT) showed the highest contents than other fractions (n-hexane, chloroform and distilled water). Antioxidant activities of EFAT has been evaluated using ABTS, DPPH radical scavenging activities, FRAP and inhibitory effect of lipid peroxidation. EFAT showed excellent radical scavenging activity and inhibitory effect on MDA production. Inhibitory effect of tyrosinase as a major enzyme of melanin synthesis was also measured. In these results, EFAT showed higher inhibitory effect against L-DOPA (51.27%) than L-tyrosine. IC50 value on α-glucosidase was 41.93 ㎍/㎖. In B16/F10 melanoma cells, EFAT inhibited melanin synthesis at 200 ㎍/㎖ concentration (about 42% decrease). Finally, main physiological compounds of EFAT were identified as a rutin and a chlorogenic acid using high performance liquid chromatography.

• 유슬기(경상대학교 농업생명과학대학 농화학식품공학과) | Seul Ki Yoo (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
• 박선경(경상대학교 응용생명과학부(BK21 plus)) | Seon Kyeong Park (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 강진용(경상대학교 응용생명과학부(BK21 plus)) | Jin Yong Kang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 김종민(경상대학교 응용생명과학부(BK21 plus)) | Jong Min Kim (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 박상현(경상대학교 응용생명과학부(BK21 plus)) | Sang Hyun Park (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 권봉석(경상대학교 응용생명과학부(BK21 plus)) | Bong Seok Kwon (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 이창준(경상대학교 응용생명과학부(BK21 plus)) | Chang Jun Lee (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 강정은(경상대학교 응용생명과학부(BK21 plus)) | Jeong Eun Kang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 박수빈(경상대학교 응용생명과학부(BK21 plus)) | Su Bin Park (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
• 이욱(국립산림과학원 특용사원연구과) | Uk Lee (Division of Special Purpose Trees, National Institute of Forest Sciece)
• 허호진(1경상대학교 농업생명과학대학 농화학식품공학과, 2경상대학교 응용생명과학부(BK21 plus)) | Ho Jin Heo (1Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea 2Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea) Corresponding author