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페놀폐수 처리를 위한 Rhodococcus sp. EL-GT 와 활성슬러지를 이용한 호기성 생물막 반응기의 미생물 군집 동태 KCI 등재
Dynamics of Microbial Community of Aerobic Biofilm Reactor using Rhodococcus sp. EL-GT and Activated Sludge for Phenol Wastewater Treatment
pp.239-245
This research was performed to investigate the dynamics of microbial community by RBC (Rotating Biological Contactor) using Rhodococcus sp. EL-GT and activated sludge. Cell counts revealed by DAPI were compared with culturable bacterial counts from nutrient agar. Colony counts on nutrient agar gave values 20∼25% and 1∼15% of cell counts (DAPI). The cell counts for the dynamics of bacterial community were determined by combination of in situ hybridization with fluorescently-labelled oligonucleotide probes and epifluorescence microscopy. Around 90∼80% of total cells visualized by DAPI were also detected by the bacteria probe EUB 338. For both reactors proteobacteria belonging to the gamma subclass were dominant in the first stage (1 and 2 stage) and proteobacteria belonging to the gamma subclass were dominant in the last stage (3 and 4 stage).
