This study constructed a wheat-specific primer and a probe using the internal transcribed spacer (ITS). 2 regions of wheat (Triticum aestivum) and real-time PCR conditions were established. The calibration curve showed a slope of -3.356, a correlation coefficient of 0.998, and an amplification efficiency of 98.589%. Experiments were carried out on the rice flour mixed with 50%, 10%, 1%, 0.1%, 0.01%, and 0.001% of wheat. The result showed that it was possible to detect samples mixed with up to 0.01% of wheat. As a result of checking, the wheat detection potential of rice, 34 processed foods, and seven processed foods was ascertained. The real-time PCR method using the wheatspecific primer and probe developed in this study can be used to identify the authenticity of the raw materials, such as the incorrect indication of the raw materials utilized and the unintended mixing of wheat during the manufacturing process.

Abstract
서 론
재료 및 방법
    재료 및 분석기기
    유전자추출
    염기서열 분석 및 프라이머 제작
    유전자 증폭
    밀 유전자 표준품
결과 및 고찰
요 약
References

• 신상운(경기도보건환경연구원 보건연구기획팀) | Sang-Woon Shin (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment) Corresponding author
• 김영숙(경기도보건환경연구원 보건연구기획팀) | Young-Sug Kim (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 김양희(경기도보건환경연구원 보건연구기획팀) | Yang-Hee Kim (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 박은미(경기도보건환경연구원 보건연구기획팀) | Eun-Mi Park (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 조상훈(경기도보건환경연구원 보건연구기획팀) | Sang-Hoon Cho (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 엄경숙(경기도보건환경연구원 보건연구기획팀) | Kyoung-Suk Eum (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 강효정(경기도보건환경연구원 보건연구기획팀) | Hyo-Jeong Kang (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)
• 윤미혜(경기도보건환경연구원 보건연구기획팀) | Mi-Hye Yoon (Public Health Research Planning Team, Gyeonggi-Do Institute of Health and Environment)