Purpose: This study aimed to investigate the readiness for interprofessional education and educational needs, develop a pediatric nursing clinical practicum program for interprofessional education, and evaluate its effectiveness. Methods: A survey of total 272 undergraduate nursing students and medical students and a focus group interview were used to explore the need of interprofessional education. An interprofessional pediatric nursing practicum program was developed. The program consisted of three sessions: orientation and ice break (1 h), holo-patient assessment (1 h), and 5 twins team-based learning (2 h). Effectiveness was evaluated among nine nursing and medical students. Data were analyzed by descriptive statistics, the independent t-test, and the Wilcoxon rank-sum paired t-test. Results: The scores of readiness for interprofessional learning in nursing students were significantly higher than those in medical students. And the scores significantly increased after participating in the program (Z=-2.81, p=.005). The overall satisfaction with the program was 4.33 out of 5 points. Conclusion: The interprofessional education program had a positive effect on the readiness for interprofessional learning. This program can have a positive impact on patient safety and quality of care and can contribute to the expansion of nursing education through various teaching methods.