논문 상세보기
pp.187-198
Opuntia ficus-indica (OFI), or Pricky Pear Cactus, is effective in cough, fever, pain and anti-inflammatory action, and asthma. This study aims to investigate the effect of OFI stem extract on the respiratory system of animal models induced by ovalbumin (OVA) and fine dust (PM10) and to analyze the indicator substances of OFI stem extract. In the OFI stem 50% ethanol extract (OFI-50E) administration group, the number of immune cells and inflammatory cytokines in the lungs and BAL decreased to a similar level to the positive control group administered with dexamethasone. In addition, OVA-specific IgE and airway hyper-reactivity (AHR) were significantly reduced. Also, the deposition of PM10 observed through staining of lung tissue was clearly reduced in the OFI-50E 200 mg/kg administration group. The anti-inflammatory mechanism in the lung was found to obstruct the production of inflammatory cytokines by impeding the NF-kB and MAPK pathways through the inhibition of IRAK-1 active cells. The main component of OFI stem 50% ethanol extract was identified to be narcissin. According to the study results, OFI is expected to be a respiratory health functional food.
서 론
재료 및 방법
손바닥선인장 줄기 추출물(OFI extract) 및 미세먼지복합물(FDM)의 제조
HPLC 분석
호흡기 손상 복합동물모델 제작
폐 조직, 기관지 폐포에서의 세포 분리
염증성 cytokine 발현 정도 분석
세포 수 산정
Western blot
면역형광염색(immunofluorescence staining)
폐조직의 조직병리학적 분석
기도 저항성(AHR; airway hyper-reactivity) 측정
Narcissin의 세포독성 측정
통계처리
결 과
OFI-50E의 HPLC 분석
기관지 폐포세척액 및 폐의 총세포수
기관지 폐포세척액 및 폐의 면역세포수
기관지 폐포세척액, 비장 세포 배양액에서의 cytokine
기도저항성 및 Ova-specific Immunoglobulin E
폐조직 검사 분석
폐조직에서 신호전달 IRAK1 단백질 발현 분석
NF-B 및 MAPK pathway 분석
Narcissin의 세포 독성
NO 생성량, IL-6 및 TNF- 생성량
요 약
감사의 글
References
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