To investigate the diversity of industrially useful microorganisms from fermented agricultural products in the Sacheon region, 128 strains were isolated and analyzed phylogenetically. The analysis indicated that the isolates were categorized into three phyla, eight families, 13 genera, and 33 species. The Bacillaceae family, which was the main taxon, comprised 88.3% of the isolates and included four genera and 22 species: Bacillus, Exiguobacterium, Fictibacillus, and Metabacillus. The Caryophanaceae family, which was the second most abundant taxon, comprised 3.9% of the isolates, including two genera and two species: Rummeliibacillus and Sporosarcina. The isolates were examined for hydrolytic enzyme activity (protease, amylase, and lipase), and 118 strains (92.2%) exhibited at least one of these enzyme activities. Furthermore, auxin production was observed in all seven strains. This study demonstrates that the isolated strains have potential applications in the food and agricultural industries in South Korea, highlighting the importance of genetic resources.