이 연구에서는 저수온(0–4℃), 고수온(30–36℃), 저염분(5–15) 조건에서 돌비늘백합 (Mercenaria mercenaria)의 생존력과 생리 반응을 종합 평가하였다. 저수온에서 누적 폐사는 15% 미만(LT50: 53.1–85.1일)으로 우수했고, 고수온은 33캜 이상에서 급격한 폐사(LT50: 36℃ 기준 0.8일)를 보였다. 저염분에서는 염분 15 까지는 생존이 가능하나, 염분 10 이하에서는 폐사가 급증하였다(LT50: 14.1–15.7일). 극한 환경에서 면역 기능이 저하되고, 탄수화물 함량 감소 등 에너지 고갈 징후도 나타났다. 이 결과는 돌비늘백합이 국내 해역의 계절 변화에 충분히 적응할 수 있음을 시사하지만, 33℃ 이상과 염분 10 이하의 환경에서는 생존 관리가 필요함을 시사한다.

The hard clam, Mercenaria mercenaria, has emerged as a promising candidate for aquaculture diversification in Korean coastal waters, particularly given the increasing environmental challenges posed by climate change. This study comprehensively evaluated the survival limits and physiological responses of M. mercenaria under extreme environmental conditions including low temperature (0-4°C), high temperature (30-36°C), and low salinity (5-15 psu) stress. Mortality rates, condition index, total carbohydrate content, and hemocyte immune parameters were assessed. Under low temperature, cumulative mortality remained below 15% across all treatment groups, with estimated LT50 values ranging from 53.1 to 85.1 days, demonstrating excellent cold tolerance. High temperature experiments revealed distinct survival thresholds, with 30°C showing high survival rates while temperatures ≥33°C resulted in rapid mortality (LT50 = 0.8 days at 36°C). Low salinity tolerance experiments indicated survival capability down to 15 psu, but severe mortality occurred below 10 psu (LT50 = 14.1-15.7 days). Hemocyte immune responses showed functional impairment under extreme conditions (≥33°C: decreased phagocytosis rate, increased oxidative stress). Condition index remained stable across most treatments, while carbohydrate content decreased significantly under extreme stress conditions (5 psu, ≥35°C), indicating energy depletion. These findings demonstrate that M. mercenaria possesses sufficient environmental tolerance to survive seasonal variations in Korean coastal waters. However, extreme conditions such as temperatures exceeding 33°C or salinities below 10 psu may pose significant risks.

서 론
재료 및 방법
    1. 실험설계 및 생물관리
    2. 누적폐사율 및 반수치사일 산정
    3. 비만도 및 총 탄수화물 함량
    4. 혈구면역력
    5. 통계
결 과
    1. 누적폐사율 및 반수치사일(LT50, Lethal Time50%)
    2. 비만도 및 총 탄수화물 함량
    3. 혈구면역력 변화
고 찰
요 약
사 사
참고문헌

• 정희도(국립수산과학원 양식연구과) | Hee-Do Jeung (Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Korea)
• 김민경(국립수산과학원 갯벌연구센터) | Mingyeng Kim (Tidal Flat Research Center, National Institute of Fisheries Science (NIFS), Gunsan 54042, Korea)
• 안현미(국립수산과학원 갯벌연구센터) | Hyun-Mi Ahn (Tidal Flat Research Center, National Institute of Fisheries Science (NIFS), Gunsan 54042, Korea)
• 김유철(국립수산과학원 갯벌연구센터) | Yu-Cheol Kim (Tidal Flat Research Center, National Institute of Fisheries Science (NIFS), Gunsan 54042, Korea)
• 황운기(국립수산과학원 갯벌연구센터) | Un-Ki Hwang (Tidal Flat Research Center, National Institute of Fisheries Science (NIFS), Gunsan 54042, Korea)
• 조영관(국립수산과학원 갯벌연구센터) | Young-Ghan Cho (Tidal Flat Research Center, National Institute of Fisheries Science (NIFS), Gunsan 54042, Korea) Corresponding author
• 강현실(국립수산과학원 아열대수산연구소) | Hyun-Sil Kang (Subtropical Fisheries Research Institute, National Institute of Fisheries Science (NIFS), Jeju 63068, Korea)