Haploids are plants with a gametophytic number of chromosomes in their sporophytes. Androgenesis occurs from asymmetric division of pollen grains into generative cells and vegetative cells, followed by re-entry of the vegetative cell during S-phase, which causes microspores progress into G2/M transition in culture. One of the most interesting features of haploids is the possibility to produce doubled haploid (DH) individuals. Doubled haploidy is extremely useful to plant breeders because it enables shortened breeding periods and efficiency in selection of useful recessive agronomic traits. Doubled-haploid technology is not only applicable to breeding, but also to transformation programs of desired genes. In addition to practical breeding programs, DH lines provide useful materials of fundamental genetics including exploitation of QTLs and genes conferred with various agronomic traits by establishing DH populations. This paper provides historical overviews on androgenesis and describes several mechanisms associated with pollen embryogenesis, including mode of actions in pollen embryogenesis, mechanisms of chromosome doubling and factors affecting androgenesis. We also discuss recent progress in application of haploids to breeding, genes associated with in vitro response and drawbacks to anther culture for application of doubled haploids in crop breeding.

AtSAGT1 encodes a salicylic acid (SA) glucosyltransferase enzyme that catalyzes the formation of SA glucoside and SA glucose ester. Here, the AtSAGT1 gene expression patterns were determined in AtSAGT1 promoter::GUS transgenic Arabidopsis plants. As a result, the factors regulating the induction of AtSAGT1 were identified as pathogen defense response, wound response, exogenous application of SA, and jasmonic acid treatment.

The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15.

An efficient plant regeneration protocol is developed for a standard-type chrysanthemum. When petal segments derived from flower buds (4 or 8cm in diameter) were used as the culture material, the highest shoot regeneration frequency (96%) was obtained on a Murashige and Skoog (MS) medium supplemented with 0.5 mg/L IAA, 2 mg/L BA, 3% sucrose, and a 0.8% agar. Pre-culturing the explants under dark conditions for 14 days produced better results for the shoot regeneration frequency than the explants cultured under a continuous 16 h photoperiod (40 μmolm-2 s -1 ). The shoot regeneration frequency ranged from 19.0% for the Shinmato cultivar to 89.1% for the Baeksun cultivar. Activated charcoal (0.2%) enhanced the root formation of the regenerated shoots in a hormone-free MS medium. The rooted plantlets were acclimatized and successfully established in a greenhouse.

This study was undertaken to determine free radical scavenging capacity and oxidative DNA damage protecting activity of methanol extract of red tea stem. The extract was subjected to assess their antioxidant potential using various in vitro systems such as DPPH•, ABTS•+ , super oxide and nitric oxide free radicals and it exhibited IC50 values of 68.88 ± 1.1, 12.08 ± 0.65, 404.38 ± 1.6, 93.6 ± 2.7, µg/mL respectively. Red tea extract also showed ferric reducing ability (FRAP) with 2606.85 mmol Fe (II)/g of extract. Furthermore, Methanol extract of red tea stem showed significant DNA damage protecting activity in concentration dependent manner against H2O2+UV induced photolysis on pUC19 plasmid DNA. Results of this study showed that the methanol extract of Red Tea stem has strong antioxidant potential along oxidative DNA damage protecting capacity that would be the significant sources of natural antioxidants, which might be helpful in preventing the progress of various oxidative stress generated diseases. Further study is necessary for isolation and characterization of the active antioxidants, which may serve as a potential source of natural antioxidant.

Nuts are one of the most common sources of allergies in individuals of all ages. In order for a particular protein to render an allergic reaction, it must resist proteolytic digestion by intestinal enzymes. In this study, three well-known allergenic nuts, almonds, cashew nuts, and peanuts, were used as samples, and enzyme digestion with Bacillus protease and porcine pepsin was tested. A proteomic approach using two-dimensional gel electrophoresis and an MS/MS analysis was applied to visualize and identify the proteins that were resistant to enzyme digestion. Among the 150 protein spots tested, 42 proteins were assigned functions. Due to the lack of genomic databases, 41% of the identified proteins were grouped as hypothetical. However, 12% of them were well-known allergens, including AraH. The remainder were grouped as storage, enzymes, and binding proteins.

The carbon-offset mechanism based on forest management has been recognized as a meaningful tool to sequestrate carbons already existing in the atmosphere. Thus, with an emphasis on the forest-originated carbon-offset mechanism, the accurate measurement of the carbon stock in forests has become important, as carbon credits should be issued proportionally with forest carbon stocks. Various remote sensing techniques have already been developed for measuring forest carbon stocks. Yet, despite the efficiency of remote sensing techniques, the final accuracy of their carbon stock estimations is disputable. Therefore, minimizing the uncertainty embedded in the application of remote sensing techniques is important to prevent questions over the carbon stock evaluation for issuing carbon credits. Accordingly, this study reviews the overall procedures of carbon stock evaluation-related remote sensing techniques and identifies the problematic technical issues when measuring the carbon stock. The procedures are sub-divided into four stages: the characteristics of the remote sensing sensor, data preparation, data analysis, and evaluation. Depending on the choice of technique, there are many disputable issues in each stage, resulting in quite different results for the final carbon stock evaluation. Thus, the establishment of detailed standards for each stageis urgently needed. From a policy-making perspective, the top priority should be given to establishinga standard sampling technique and enhancing the statistical analysis tools.

환경변화 중요성과 유전적 안전성은 최근에 증가추세의 형질전환 작물에 인식되고 있다. 본 실험은 건조저항성형질전환체 작물의 유전적인 안전성과 환경변이에 따른 농업적인 특성을 분석하였다. 내건성 형질전환체인CaMsrB2-8, 23 과 모품종인 ‘일미’ 및 일반품종을 대조구로GM필드에서 작물학적 생육특성을 조사하였다. 농업적인생육 특성에서 CaMsrB2-8, 23 계통은 모품종인 ‘일미’와년차간, 지역별 평균으로 표현형은 유사하였다. 수량에서 년도별, 지역별 차이는 있지만 통계적인 유의성은 없었다.현미의 미립특성에서 CaMsrB2-8, 23은 모품종인 ‘일미’와차이를 보이지 않았다. 현미의 화학적 성분 분석에서CaMsrB2- 2 계통의 전분과 단백질함량은 모품종인 ‘일미’보다 일반품종인 ‘일품’ 화학적 성분함량이 유사하였다.본 실험결과에서 내건성 형질전환체인 CaMsrB2-8, 23는GM 작물의 후대에서도 유전적인 안전성과 함께 수행될수 있을 것으로 사료된다.

농용무인 헬리콥터는 벼농사는 물론 전작, 과수 등 소규모 필지의 정밀방제에 이용되고 있으며 농작업의 새로운 패러다임으로 자리 잡고 있다. 본 연구의목적은 농용헬리콥터의 비상시퀀스에서 정지비행 중 측풍에 의해서 비상착륙자리를 이탈하지 않고 위치를 유지하는 편류제어 모듈과 알고리즘의 성능을 평가하는데 있다편류제어의 목적은 비상착륙과 연동되어있는데, 비상조건에 도달하게 되면 호버링을 하면서 비상대처 알고리즘이 작동하게 된다. 그러나 관성 제어 는등속운동에서기체의 움직임을 감지하지 못하게 되고 측풍에 의하여 비상지점으로부터 편류를 하게 되어 착륙 목표지점으로부터 멀어질 수 있다.GPS 모듈을 기초로 개발한 편류제어모듈을 시험하였다.알고리즘 및 실효성을 고려하여 5 m 직경 내에 위치를벗어나지 않는지에 대한 기준을 적용하였다. 초기에는 2~4m/s의 측풍 교란에 대하여 과민하게 반응하였지만 이후5 m직경 내에서 위치를 벗어나지 않고 자세 및 요의 방향을 유지 하였다. 목도의 관찰에서는 전후좌우 흔들림을인지하기 어려운 정도이지만, 데이터에서 보인 편위는GPS 수신기의 특성에 기인하는 것으로 판단한다. 이와같은 편류제어는 비상착륙이나 호버링을 유지하려 할 때의도하지 않는 편류를 제어하는데 사용될 수 있다.

This study analyzed agricultural water distribution systems for the utilization of water demand-oriented water supply systems. Three major TM/TC(telemeter/telecontrol) districts of agricultural water management were selected for analyzing the characteristics of the water distribution systems. In addition, the characteristics of the water supply systems for general water supply zones based on irrigation facilities were also investigated, along with the case of special water management during the drought season. As a result, high annual and monthly variations were observed for the water supply facilities, including the reservoirs and pumping stations. In particular, these variations were more obvious during the drought season, depending on the type of facility. The operations of the pumping stations and weirs were more sensitive to the stream levels than the reservoirs, and the smaller reservoirs were influenced more than the larger reservoirs. Therefore, a water-demand-oriented water supply system should consider the existing general practices of water management in the agricultural sector, and focus on achieving a laborsaving system rather than water conservation in the case of reservoirs. Equal water distribution from the start to the end point of irrigation channels could be an effective solution for managing pumping stations.