This study investigated the relationship among seven species of trichoderma through the identification of strains collected in Korea. The phylogenetic tree among the collected strains was classified into four groups. The trichoderma strains isolated in this way showed inhibitory effect on the fusarium wilt which is parasitic to cotyledon stem..The invisibility of J9, J10, J13 and J16 strains were higher in comparison with other strains in vitro test stand, and their spore production level was also higher. In the aluminum ring tests, it showed that the yield of the spores in J9, J10 and J13 were more than any other strain. As a result conducting the port test for cucumbers, the plant lengths of J13 were larger than the control plot, and the root lengths of all strains, except for J2 were longer than the control plot, and the root weights of J1, J9, J10, J13 and J16 were larger than the control plot. The disease severity for the fusarium wilt showed the smallest values at J13 and J16 in comparison with the control plot, and the control values of J13 and J16 were higher than other strains.

This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

This experiment was conducted to assess changes to the chemical properties of soil and applicability in a case of rice cultivation with organic fertilizers. The investigator applied organic fertilizers to rice cultivation for five years to examine changes to the chemical properties of soil and found that the experiment group of organic fertilizers made an ongoing increase in pH, organism content, and available phosphate content annually compared with the control group with no big differences according to the amounts of organic fertilizers used. As for the yield components, there were no statistical differences in the number of spikelets and grain filling rate between the experiment group of organic fertilizers and the control group. The experiment group recorded a higher level in 1,000 seeds weight and yield than the control group. Experiment Group 4 recorded the highest level at 29.11 kg of 1,000 seeds weight. Experiment Groups 3 and 4, which used 222 kg and 267 kg per 10 a, respectively, recorded 576 kg and 572 kg of yield, respectively, which were 4.7% and 4.1% higher than 549 kg of control group, respectively. As for the quality of brown rice, there were no statistical differences in the head rice percentage between the control and experiment group, both of which were in the range of 83.2-85.7%. As for the protein content, Experiment Groups 3 and 4, both of which used a lot of organic fertilizers, were in the range of 6.9-7.1%, which was lower than 7.5% of control group. Those findings indicate that the long-term application of organic fertilizers can improve the chemical properties of soil and increase the yield more than the conventional method of fertilizer application. The findings also suggest that it will be effective to apply 222 kg of organic fertilizers or more per 10 a.

his study was carried out to investigate antimicrobial activities of water extracted Pleurotus ostreatus var Heuktari (PO), and their application to F. fujikuroi Nirenberg growth inhibition material. Various organic solvents (chloroform, hexane, ethyl acetate, ethanol) were tested to investigate the antimicrobial activities of Pleurotus ostreatus var Heuktari against F. fujikuroi Nirenberg. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but butanol extract showed 2.5% strong activities in order of F. fujikuroi Nirenberg disk diffusion test. Then we observe Antifungal activity using green house. Bed soil and mushroom extract mixed at 10%, 7.5%, 5%, 2.5%, 1% total bed soil mass. Screening of Antifungal activity was tested two periode 18day and 25day. The Antifungal activity rate of each period and extract density was 7.5% mixed bedsoil has investigated 80% Antifungal activity rate. The result indicates that the Pleurotus ostreatus var Heuktari extract using F. fujikuroi Nirenberg registance, which shows the development of organic agriculture seed disinfectant.