We have collected dozens of mid-infrared spectra showing UIR bands from diffuse Galactic emitting regions with the AKARI's Infrared Camera (IRC) onboard AKARI, as part of the ISMGN Mission Program. The datasets cover various directions in the inner Galactic Plane ( |l| < 70 deg), in the outer Galactic Plane ( |l| > 70 deg), and in the off-Plane ( |b| > 2 deg). The variations in the UIR band ratios are examined in terms of the radiation environments judged from the far-infrared ( 50 − 170 μm ) spectral energy distribution (SED) made with AKARI/FIS All Sky Survey data at each slit position where mid-IR spectra were obtained. We have found that the band ratios of 6.2 μm / 11.2 μm and 7.7 μm / 11.2 μm toward the inner Galaxy are systematically higher than those toward the outer Galaxy and off the Galactic plane. Likely causes of the variations in properties of UIR bands in diffuse emission on a Galactic scale are discussed in this paper.

We present the results of the near-infrared (NIR) to mid-infrared (MIR) slit spectroscopic observations of the diffuse emission toward nine positions in the nearby irregular galaxy Large Magellanic Cloud (LMC) with the Infrared Camera (IRC) on board AKARI. The unique characteristic of AKARI/IRC provides a great opportunity to analyze variations in the unidentified infrared (UIR) bands based on continuous spectra from 2.5 to 13.4 μm of the same slit area. The observed variation of I3.3 / I11.3 suggests destruction of small-sized UIR band carriers, polycyclic aromatic hydrocarbons (PAHs) in harsh environments. This result demonstrates that the UIR 3.3 μm band provides us powerful information on the excitation conditions and/or the size distribution of PAHs, which is of importance for understanding the evolutionary process of hydrocarbon grains in the Universe. It also suggests a new diagnostic diagram of two band ratios, such as I3.3 / I11.3 versus I7.7 / I11.3 , for the interstellar radiation conditions. We discuss on the applicability of the diagnostic diagram to other astronomical objects, comparing the LMC results with those observed in other galaxies such as NGC 6946, NGC 1313, and M51.

The previous studies of p16^INK4a, pRb, p53, and Ki-67 expression suggested that these markers may be preferentially expressed in cervical neoplasms. The purpose of this study was to assess the expression and the clinical significance of p16^INK4a, pRb, p53, and Ki-67 proteins in cervical lesions. We obtained 106 cases with various categories of cervical squamous mucosa, including squamous cell carcinoma (n=35), cervical intraepithelial neoplasia (CIN) II/III (n=26), CIN I (n=10), squamous metaplasia (n=15), and normal squamous mucosa (n=20). Immunohistochemical staining was performed for p16INK4a, pRb, p53, and Ki-67 proteins in formalin-fixed and paraffin-embedded tissue sections of the uterine cervix. Evaluation of immunohistochemical staining was based on the frequencies of expression and the mean immunoreactivity scores (IS) in each diagnostic category. p16INK4a positive sotaining was observed in 26 of 35 cases (74.3%) of squamous cell carcinoma, in 16 of 26 cases (61.5%) of CIN II/III, in six of 10 cases (60%) of CIN I, in nine of 15 cases (60%) of squamous metaplasia, and negative in normal squamous mucosa. pRb expression was detected in all diagnostic categories; however, the proportion of pRb positive cells was relatively decreased in CIN II/III (38.5%) and squamous cell carcinomas (51.4%), compared to normal squamous epithelium (90%) and squamous metaplasia (73.3%). No significant differences in expression of p53 were observed in any diagnostic categories. Ki-67 expression was increased in squamous cell carcinoma (37.1%), CIN II/ III (42.3%), and CIN I (40%), but negative in squamous metaplasia and normal mucosa. In 35 cases of squamous cell carcinomas, multivariate analysis revealed no differences in p^INK4a, pRb, p53, and Ki-67 expression according to the age of the patient, lymph node metastasis and clinical stage. In conclusion, the combined use of p16^INK4a and Ki-67 immunoreactivity could improve the diagnostic specificity of squamous cell carcinoma of the uterine cervix.

The shortage of human organs for transplantation has induced the research on the possibility of using animal as porcine. However, pig to human transplantation as known as xeno-transplantation has major problem as immunorejection. Recently, the solutions of pig to human xenotransplantation are commonly mentioned as having a genetically modification which include alpha 1, 3 galatosyl transferase knockout (GTKO) and immune-suppressing gene transgenic model. Unfortunately, the expression level of transgenic gene is very low activity. Therefore, development of gene overexpression system is the most urgent issue. Also, the tissue specific overexpression system is very important. Because most blood vessels are endothelial cells, establishment of the endothelial-specific promoter is attractive candidates for the introduction of suppressing immunorejection. In this study, we focus the ICAM2 promoter which has endothelial-specific regulatory region. To detect the regulatory region of ICAM2 promoter, we cloned 3.7 kb size mini-pig ICAM2 promoter. We conduct serial deletion of 5' flanking region of mini-pig ICAM2 promoter then selected promoter size as 1 kb, 1.5 kb, 2 kb, 2.5 kb, and 3 kb. To analyze promoter activity, luciferase assay system was conducted among these vectors and compare endothelial activity with epithelial cells. The reporter gene assay revealed that ICAM2 promoter has critical activity in endothelial cells (CPAE) and 1 kb size of ICAM2 promoter activity was significantly increased. Taken together, our studies suggest that mini-pig ICMA2 promoter is endothelial cell specific overexpression promoter and among above all size of promoters, 1 kb size promoter is optimal candidate to overcome the vascular immunorejection in pig to human xenotransplantation.

Following the first Public Release of the AKARI Point Source catalogues, we have worked on the production of a new far-infrared All-Sky Diffuse mapping product. In this paper we report first results from the All Sky diffuse maps that will shortly be released to the community, based on analysis of data from the Far Infrared Surveyor ( 65 μm − 160 μm ) instrument. These data are likely to have a strong impact on studies of extended structures, and the diffuse ISM.

We show how the rotation emission from isolated interstellar Polycyclic Aromatic Hydrocarbons (PAHs) can explain the so-called anomalous microwave emission (AME). AME has been discovered in the last decade as microwave interstellar emission (10 to 70 GHz) that is in excess compared to the classical emission processes: thermal dust, free-free and synchrotron. The PAHs are the interstellar planar nano-carbons responsible for the near infrared emission bands in the 3 to 15 micron range. Theoretical studies show that under the physical conditions of the interstellar medium (radiation and density) the PAHs adopt supra-thermal rotation velocities, and consequently they are responsible for emission in the microwave range. The first results from the PLANCK mission unexpectedly showed that the AME is not only emitted by specific galactic interstellar clouds, but it is present throughout the galactic plane, and is particularly strong in the cold molecular gas. The comparison of theory and observations shows that the measured emission is fully consistent with rotation emission from interstellar PAHs. We draw the main lines of our PLANCK-AKARI collaborative program which intends to progress on this question by direct comparison of the near infrared (AKARI) and microwave (PLANCK) emissions of the galactic plane.

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, -MEM or KnockOut-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, -MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and -MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, -MEM tended to be more suitable after passage 6. Although -MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.

Oxidative stress has been reported to be one of causes of neuritis. This study examined antioxidative activities of methanol extracts of six amphibian species known to be medicinal animals (Rana catesbeiana, R. coreana, R. rugosa, R. dybowskii, R. nigromaculata, and Hyla japonica) and investigated their effects of inhibiting nitric oxide (NO) production and cytotoxicity on the murine macrophage RAW264.7 cells. As inflammation is closely associated with reactive oxygen species, assays on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, xanthine oxidase inhibitory activity, superoxide anion radical scavenging activity and NO scavenging activity of the extracts of the six species were performed to investigate their antioxidative activity. The results obtained were as follows; All extracts showed antioxidative activity, and the activity of R. dybowskii was the highest in comparison among those. Anti-inflammatory effects of the extracts were also examined, the five extracts except that of R. rugosa did not show cytotoxicity for RAW264.7 cells at the maximal concentration (1,000 μg mL-1). Selectivity index, meaning NO scavenging activity compared to cytotoxicity, showed the highest level in the extract of R. dybowskii. These results will be very useful basic data for future studies on prevention and treatment of human diseases to understand the biological roles of amphibian extracts throughout the antioxidative or anti-inflammatory pathways.

This study considered the effect of the heat treatment temperature on the compressive strength of coal powder compacts affected by density, porosity, and crystallinity. Coal powder compacts were made by pressing of milled coal powder and were heat treated at 200, 400, 600, 800, and 1000℃. The density and porosity of the heat treated specimens at each temperature were measured using the Archimedes method and changes in crystallinity were analyzed using Raman spectroscopy. Increases in compressive strength at 600℃ or higher temperatures were proportionally related to increases in the density and the degree of crystallinity.

In the past four years, outbreaks of acute respiratory diseases associated with canine influenza H3N2 viruses in dogs and cats have been reported in South Korea and China. For prevention of disease from spread of the disease and for administration of timely medical treatments, including countermeasures for quarantine, use of a rapid and highly sensitive detection method are important to detection of the causative viruses. This study was conducted in order to develop a real time RT-PCR for the H3N2 subtype. It was based on primers targeting the highly homologous sequences of matrix, hemagglutinin, and neuraminidase genes. The detection limit of real time RT-PCR was 10 copies/ul with matrix and hemagglutinin genes, and 1 copy with neuraminidase genes, respectively. This real time RT-PCR was as sensitive as virus isolation in 52 clinical samples. The detection system developed in this study might provide more rapid and highly sensitive results than commercial rapid kits based on immunochromatographic assay.

Aggregatibacter actinomycetemcomitans is the most important etiologic agent of aggressive periodontitis and can interact with endothelial cells. Monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) are chemokines, playing important roles in periodontal pathogenesis. In our current study, the effects of A. actinomycetemcomitans on the production of MCP-1 and IL-8 by human umbilical vein endothelial cells (HUVEC) were investigated. A. actinomycetemcomitans strongly induced the gene expression and protein release of both MCP-1 and IL-8 in a dose- and time-dependent manner. Dead A. actinomycetemcomitans cells were as effective as live bacteria in this induction. Treatment of HUVEC with cytochalasin D, an inhibitor of endocytosis, did not affect the mRNA up-regulation of MCP-1 and IL-8 by A. actinomycetemcomitans. However, genistein, an inhibitor of protein tyrosine kinases, substantially inhibited the MCP-1 and IL-8 production by A. actinomycetemcomitans, whereas pharmacological inhibition of each of three members of mitogen-activated protein (MAP) kinase family had little effect. Furthermore, gel shift assays showed that A. actinomycetemcomitans induces a biphasic activation (early at 1-2 h and late at 8-16 h) of nuclear factor-κB (NF-κB) and an early brief activation (0.5-2 h) of activator protein-1 (AP-1). Activation of canonical NF-κB pathway (IκB kinase activation and IκB-α degradation) was also demonstrated in these experiments. Although lipopolysaccharide from A. actinomycetemcomitans also induced NF-κB activation, this activation profile over time differed from that of live A. actinomycetemcomitans. These results suggest that the expression of MCP-1 and IL-8 is potently increased by A. actinomycetemcomitans in endothelial cells, and that the viability of A. actinomycetemcomitans and bacterial internalization are not required for this effect, whereas the activation of protein tyrosine kinase(s), NF-κB, and AP-1 appears to play important roles. The secretion of high levels of MCP-1 and IL-8 resulting from interactions of A. actinomycetemcomitans with endothelial cells may thus contribute to the pathogenesis of aggressive periodontitis.

An overview of the North Ecliptic Pole (NEP) deep multi-wavelength survey covering from X-ray to radio wavelengths is presented. The main science objective of this multi-wavelength project is to unveil the star-formation and AGN activities obscured by dust in the violent epoch of the Universe (z=0.5-2), when the star formation and black-hole evolution activities were much stronger than the present. The NEP deep survey with AKARI/IRC consists of two survey projects: shallow wide (8.2 sq. deg, NEP-Wide) and the deep one (0.6 sq. deg, NEP-Deep). The NEP-Deep provides us with a 15 μm or 18 μm selected sample of several thousands of galaxies, the largest sample ever made at these wavelengths. A continuous filter coverage at mid-IR wavelengths (7, 9, 11, 15, 18, and 24 μ m ) is unique and vital to diagnose the contribution from starbursts and AGNs in the galaxies at the violent epoch. The recent updates of the ancillary data are also provided: optical/near-IR magnitudes (Subaru, CFHT), X-ray (Chandra), FUV/NUV (GALEX), radio (WSRT, GMRT), optical spectra (Keck/DEIMOS etc.), Subaru/FMOS, Herschel/SPIRE, and JCMT/SCUBA-2.

Far-infrared observations provide crucial data for the investigation and characterisation of the properties of dusty material in the Interstellar Medium (ISM), since most of its energy is emitted between ~ 100 and 200 μm . We present the first all-sky image from a sensitive all-sky survey using the Japanese AKARI satellite, in the wavelength range 50 − 180 μm . Covering > 99% of the sky in four photometric bands with four filters centred at 65 μm , 90 μm , 140 μm , and 160 μ m wavelengths, this achieved spatial resolutions from 1 to 2 arcmin and a detection limit of < 10 MJy s r − 1 , with absolute and relative photometric accuracies of < 20%. All-sky images of the Galactic dust continuum emission enable astronomers to map the large-scale distribution of the diffuse ISM cirrus, to study its thermal dust temperature, emissivity and column density, and to measure the interaction of the Galactic radiation field and embedded objects with the surrounding ISM. In addition to the point source population of stars, protostars, star-forming regions, and galaxies, the high Galactic latitude sky is shown to be covered with a diffuse filamentary-web of dusty emission that traces the potential sites of high latitude star formation. We show that the temperature of dust particles in thermal equilibrium with the ambient interstellar radiation field can be estimated by using 90 μm , 140 μm , and 160 μm data. The FIR AKARI full-sky maps provide a rich new data set within which astronomers can investigate the distribution of interstellar matter throughout our Galaxy, and beyond.

Environmental problems such as global warming become social issues today. It is reported that increasing energy consumption has affected on these environmental issues. Buildings deploys 40 percent of the raw materials in the world while at the same time they emit over 35 percent of the total CO2, so that it utilize over 50 percent of the whole energy in the world. At this point, energy saving though effective building control may eventually bring social, environmental and economical effects. Thus, this study will explore the difference between measured temperature and monitored temperature for operating air control system in the building, to which BEMS is applied. Also, it will suggest a method to accurately predict the load of air-conditioning, and will examine the different amounts of the load according to the temperature as well as the reduced amount of the load through changes of operation standard temperature.

This study deals with an enhanced assumed strain (EAS) three-dimensional element for free vibration analysis of laminated composite and sandwich structures. The three-dimensional finite element (FE) formulation based on the EAS method for composite structures shows excellence from the standpoints of computational efficiency, especially for distorted element shapes. Using the EAS FE formulation developed for this study, the effects of side-to-thickness ratios, aspect ratios and ply orientations on the natural frequency are studied and compared with the available elasticity solutions and other plate theories. The numerical results obtained are in good agreement with those reported by other investigators. The new approach works well for the numerical experiments tested, especially for complex structures such as sandwich plates with laminated composite faces.

This study was performed to compare the muscle activity of lumbar stabilizers between stoop and semi-squat lifting techniques at different lifting loads. Twenty healthy subjects (9 males, 11 females) were recruited for this study. Muscle activity of external obliques (EO), internal obliques (IO) and lumbar multifidus (LM) muscle was measured by surface electromyography during stoop and semi-squat lifting at different lifting loads (10%, 20%, and 30% of the subject's body weight). A one-way repeated measure ANOVA was applied. The results showed that EMG activity of EO was significantly increased with a load of 30% of body weight compared to 10% and 20% of body weight in both lifting techniques (p<.05). Muscle activity of LM was significantly increased in 20% compared to 10% and 30% compared to 10% of subject's body weight in stoop lifting and the muscle activity of LM was significantly increased in 20% compared to 10%, 30% compared to 20%, and 30% compared to 10% of the subject's body weight in semi-squat lifting (p<.05). However, there was no significant difference in activity of IO according to lifting loads in both lifting techniques. There were no significant differences in muscle activity of EO, IO, and LM between stoop and semi-squat technique (p>.05). Therefore, the results of this study suggested that the EO can contribute to increase the lumbar stability during stoop and semi-squat lifting at 30% of body weight rather than at lower loads, and the LM seems to act as counteractor to imposed loads during stoop and semi-squat lifting with increasing loads.

Morphological control on hydroxyapatite crystals has attractive prospects in research to clarify the effects of crystal planes on biological performance. Hydrothermal processing is known as a typical type of processing for fabricating well-grown crystals with unique morphology. The purpose of the present study is to examine the feasibility of well-crystallized crystals with oriented structures through hydrothermal treatment of calcite. A single crystal of calcite was applied to hydrothermal treatment in a phosphate solution at 160˚C. Rod-shaped hydroxyapatite crystals with micrometer-size were formed on the 100 face of calcite after treatment, while nanometer-sized hydroxyapatite crystals were formed on the (111). The hydroxyapatite crystals formed on each plane were not morphologically changed with increasing treatment periods. An oriented structure of rod-shaped hydroxyapatite was constructed after hydrothermal treatment of 100 planes on the calcite single, while such orientation was not observed on the (111) plane after the treatment. The layer of hydroxyapatite formed on the 100 plane was thicker than that of the (111) plane. The 100 plane of calcite shows a higher reactivity than that of the (111) plane, which results in rapid crystal growth of hydroxyapatite. The difference in the morphology of the formed hydroxyapatite was governed by the reactivity of each crystal plane exposed to the surrounding solution.

로렌스의 <채털리 부인의 연인>은 과거에 유럽은 물론 일본, 미국에서도 센세이션을 일으켰던 유명한 서적이다. 자유스러운 인터넷, 디지털 문학이 주류가 된 오늘 현대에 있어서 음란물 소지 또는 유포 행위를 형사 처벌한다는 것은 헌법적으로 어떤 의미가 있을까. 고전문학 <채털리 부인의 연인>을 재조명하여 미국 판례를 분석해 보았다. 미국은 이탈리아에서 출간된 이 책을 강력하게 음란물로 제정했다가 1959년에서야 비로소 뉴욕 법원에서 금지령을 풀어 주었던 역사가 있다. 1928년에 발행된 이후 지난 세월 동안 음란물 유포 행위 관련 범죄를 다루는 시각이 미국에서 어떻게 변화해 왔는지, 그리고 현재 사용되는 관련 법률, 또한 유죄 판결 통계를 살펴 보았다. 미국 법원에서 <채털리 부인의 연인> 이후로도 음란물 유포 행위는 종종 형사 처벌의 대상이 되어 왔는데, 시대와 장소에 따라서 변화한다는 것을 알 수 있다. 이것은 미국이 지역적 기준(community standard)으로 볼 때 음란물이어야 한다는 법적 기준을 유지하고 있는데 기인한다. 예를 들면 2012년에 뉴욕 맨하탄 시민의 기준으로 음란물을 보는 시각은 1950년대 캔자스 지역에서 옥수수 농사를 짓는 배심원들의 시각과는 현저히 다를 수 있다는 것이다. 음란물의 유포 행위를 범죄화 하는 법제 자체가 이제는 지나간 시대의 산물이라고 말하는 이들도 종종 있지만, 아직까지 음란물은 미국에서 헌법의 보호를 받을 수 있는 언론의 자유 행사 행위에 포함되지 않는다는 것이 대법원의 의견이다.