There are many difficulties to supply constant power to marine facilities which operate in the sea. Especially, there is a limit to stand alone power supply systems due to the influence of weather conditions. That's why a hybrid power supply system is required to overcome these problems. This paper will describe an Electronic Anti-Fouling System (EAFS) to maximise the power efficiency for a solar - wave hybrid power generation system. A main factor reducing the efficiency of a Wave Energy Converter (WEC) is due to the attachment of aquatic life forms. Therefore the aim of this research is to develop a simulation programme to enable the design of more efficient EAFS for hybrid power generation systems and to provide valuable data for production of more efficient EAFS.

Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for F_1-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in F_1-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating F_1-hybrid cultivar development in radish.

In an attempt to simultaneously produce two human proteins, hGH and hG-CSF, in the milk of transgenic mice, we constructed goat -casein-directed hGH and hG-CSF expression cassettes individually and generated transgenic mice by co-injecting them into mouse zygotes. Out of 33 transgenic mice, 29 were identified as double transgenic harboring both transgenes on their genome. All analyzed double transgenic females secreted both hGH and hG-CSF in their milks. Concentrations ranged from 2.1 to for hGH and from 0.04 to for hG-CSF. hG-CSF level was much lower than hGH level but very similar to that of single hG-CSF mice, which were introduced with hG-CSF cassette alone. In order to address the causes of concentration difference between hGH and hG-CSF in milk, we examined mRNA level of hGH and hG-CSF in the mammary glands of double transgenic mice and tissue specificity of hG-CSF mRNA expression in both double and single transgenic mice. Likewise protein levels in milk, hGH mRNA level was much higher than hG-CSF mRNA, and hG-CSF mRNA expression was definitely specific to the mammary glands of both double and single transgenic mice. These results demonstrated that two transgenes have distinct transcriptional potentials without interaction each other in double transgenic mice although two transgenes co-integrated into same genomic sites and their expressions were directed by the same goat -casein promoter. Therefore goat -casein promoter is very useful for the multiple production of human proteins in the milk of transgenic animals.

고준위폐기물 처분장의 기본적인 기능은 단기간에 과도한 방사성핵종이 유출되는 것을 방지하는데 있다. 이를 위해서는, 처분시스템의 구성요소들과 관련된 많은 기술 기준이 개발되고 수립되어야 한다. 처분장 안전성평가는 합리적으로 단순화된 모델에 바탕을 두고 처분장의 미래 전개에 대한 정량적인 결과를 산출하므로 안전성평가는 기술기준의 하나로 간주되고 있다. 본문에서는 평가기간, 선량 제한치 및 평가의 불확실성 등을 중심으로 안전성평가와 관련된 주요국의 기술기준을 조사하였다. 특히, 미국의 기준을 조사하는 과정에서 안전성평가에서 도출된 peak dose가 선량 제한치를 충족하지 못하는 경우, 평가기간 및 peak dose에 대한 미국 규제당국의 접근방안은 참고할만한 가치가 있음을 알 수 있었다.

In mammals, puberty is a dynamic transition process from infertile immature state to fertile adult state. The neuroendocrine aspect of puberty is started with functional activation of hypothalamus-pituitary-gonadal hormone axis. The timing of puberty can be altered by many factors including hormones and/or hormone-like materials, social cues and metabolic signals. For a long time, attainment of a particular body weight or percentage of body fat has been thought as crucial determinant of puberty onset. However, the precise effect of high-fat (HF) diet on the regulation of hypothalamic GnRH neuron during prepubertal period has not been fully elucidated yet. The present study was undertaken to test the effect of a HF diet on the puberty onset and hypothalamic gene expressions in immature female rats. The HF diet (45% energy from fat, HF group) was applied to female rats from weaning to around puberty onset (postnatal days, PND 22-40). Body weight and vaginal opening (VO) were checked daily during the entire feeding period. In the second experiment, all animals were sacrificed on PND 36 to measure the weights of reproductive tissues. Histological studies were performed to assess the effect of HF diet feeding on the structural alterations in the reproductive tissues. To determine the transcriptional changes of reproductive hormone-related genes in hypothalamus, total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Body weights of HF group animals tend to be higher than those of control animals between PND 22 and PND 31, and significant differences were observed PND 32, PND 34, PND 35 and PND 36 (p<0.05). Advanced VO was shown in the HF group (PND p<0.001) compared to the control (PND ). The weight of ovaries (p<0.01) and uteri (p<0.05) from HF group animals significantly increased when compared to those from control animals. Corpora lutea were observed in the ovaries from the HF group animals but not in control ovaries. Similarly, hypertrophy of luminal and glandular uterine epithelia was found only in the HF group animals. In the semi-quantitative RT-PCR studies, the transcriptional activities of KiSS-1 in HF group animals were significantly higher than those from the control animals (p<0.001). Likewise, the mRNA levels of GnRH (p<0.05) were significantly elevated in HF group animals. The present study indicated that the feeding HF diet during the post-weaning period activates the upstream modulators of gonadotropin such as GnRH and KiSS-1 in hypothalamus, resulting early onset of puberty in immature female rats.

핵연료 운반용 실린더의 재사용을 위한 용기세척공장의 제염공정에 대한 성능을 평가하기 위하여 Na2CO3 + H2O2 혼합용액의 조합을 약간 달리한 2회의 시험을 실행하였다. 각 시험은 모두 일련의 5 단계에 걸쳐 실시되었다. 우라늄 제염의 주 화학종은 Na4UO2(CO3)3 로 식별되었다. 그리고 첫 단계에서의 세척액은 물이었으며, 이 단계에서 50% 이상의 우라늄이 제염되었다. 그 이후로는 단계가 더해 갈수록 우라늄의 제염양은 지수함수적으로 감소하는 경향을 나타내었으며, 화학양론적으로 제거된 우라늄에 비하여 투여된 Na2CO3 의 양은 과다함을 나타내었다. 이러한 결과들에 의하면, 공정최적화를 통하여 Na2CO3 의 투여량 감축, 세척폐액의 감량, 제염단계 축소 등을 꾀할 수 있을 것으로 판단된다.

In this paper, offshore wind resources within the Japan’s EEZ (Exclusive Economic Zone) are assessed using wind speed data from the microwave scatterometer SeaWinds onboard QuikSCAT. At first, from the 10m-height wind speed from QuikSCAT, 60 m-height wind speed is estimated by using an empirical equation for height correction. Based on the 60m-height wind speeds, annual energy production is calculated under an assumption of installing 2 MW wind turbines every 0.64 km2. The annual energy production is then accumulated for the entire Japan’s territorial waters and EEZ (4.47×106 km2). As a result, it is shown that the total energy production is estimated to be 4.86×104 TWh/yr. This offshore wind energy potential within the EEZ is approximately 50 times higher than the actual annual electricity production in Japan.

The effects of the cation-to-anion resin ratio and bed depth on ion exchange performance of mixed-bed were studied at ultralow solution concentration. Breakthrough curves were experimentally obtained for NaCl solution as functions of resin ratio and bed depth. The bed depth affects the pattern of the sodium breakthrough curve but not the chloride breakthrough curve in beds because of the selectivity difference. Resin selectivity determines the shape of breakthrough curves. Some sodium and chloride breakthrough curves crossed at a point as a function of resin ratio. The lower cation-to-anion resin ratio showed the higher effluent concentration or treated volume of the crossover point regardless of the total resin weight.

An experiment was conducted to find out among 9 trap settings the most appropriate site for trap placement in the Brinjal field based on Brinjal shoot and fruit borer trapping efficiency, shoot and fruit infestation, healthy and total fruit yield, and BCR. The efficiency of different trap setting positions varied significantly. Trapping efficiency observed the T1 ensured the minimum shoot and fruit infestation 10.02% and 20.95%, respectively, minimum infested fruit yield (4.75 ton/ha), maximum healthy and total fruit yield (26.72 and 31.47 ton/ha) and the maximum BCR (1.70), which was followed by T2 and T4. The minimum trapping efficiency of T9 treatment led the maximum shoot and fruit infestation 13.89 and 29.26%, respectively, maximum infested fruit yield (7.59 ton/ha), minimum healthy and total fruit yield (17.74 and 25.32 ton/ha) and the minimum BCR (1.00). A correlation between the number of BSFB adults trapped from the most efficient trap setting and the shoot and fruit infestation recorded and found a linear positive correlation between number of BSFB adults trapped and shoot infestation (r = 0.781) and fruit infestation (r = 0.810). The effect of pheromone trap positions observed in this study may be attributed to the easy accessibility of the lures and traps, when they are placed at the canopy.

The objective of this study was to characterize the main-effect QTLs, epistatic QTLs and QTL-by-environment interactions (QE), which are involved in the control of protein content. A population of 120 doubled haploid (DH) lines derived from a cross between 'Samgang' and 'Nagdong', was planted and determined for protein content over three years. Based on the population and a genetic linkage map of 172 markers, QTL analysis was conducted by WinQTLcart 2.5 and QTLMAPPER. Three main-effect QTLs affecting protein content of brown rice were detected from 2004 to 2006 on chromosomes 1 and 11. The qPC11.2 was repeatedly detected across two years. Seven pairs of epistatic loci were identified on eight chromosomes for protein content and collectively explained 39.15% of phenotype variation. These results suggest that epistatic effects might be an even more important component of the genetic basis for protein content and that the segregation of the DH lines for protein content could be largely explained by a few main-effect QTLs and many epistatic loci.

The goal of ecotourism is the conservation of nature, creating benefits for local residents, and providing high quality tourism for visitors. This study investigated: (1) visitors' satisfaction about festival activities and (2) the contribution of ecotourism to the region, specifically visitors' understanding of the local culture and visitors' consumption at this festival. The results of this study were as follows. First, spending time with family was the major reason of visiting this festival. The major visiting pattern of this festival was family, too. Developing family oriented experience programs were required. Second, visitors were quite satisfied with the programs of this festival. Visitors were the most satisfied the International Butterfly Room where visitors can see live butterflies. Third, visitors could understand the local culture in this festival and the county's development strategy is related to butterflies. Visitors could see butterflies even on blankets and pillows of motels, and designs at the bus terminal in Hampyeong county. Fourth, in terms of visitors' consumption at this festival, visitors spent more money on lodging, traffic, entrance fees. Finally, managerial implications for the festival staff were suggested.