With the rapid development of information technology, e-commerce is gradually changing people's production and lifestyle, changing the way of international trade; even the system and mechanism of international trade are changed by e-commerce, and new business models are created. E-commerce not only creates more trade opportunities for enterprises in various countries, but also shortens the trade distance and trading time of trade. It makes transactions unrestricted by geographic location and natural conditions, and the simplification of traditional trading procedures also increases transaction efficiency. It achieves truly global, intelligent and paperless transactions. This paper further expands the influence of e-commerce on international trade from inter-enterprise trade to individual-enterprise transactions, and analyzes the impact from the perspective of influencing mechanism. On the basis of theoretical analysis, combined with the development of international trade and the status of the promotion of e-commerce to trade development. From the aspects of trade mode, trade subject, trade management, a new elaboration has been made. From the perspective of influencing mechanism, the spatial aggregation of trade, commercial platform, trade flow, business model, information flow and business flow were systematically analyzed.

Along with the economic globalization and network generalization, this provides a good opportunity to the development of cross-border ecommerce trade. Based on this background, this paper sets ASEAN countries as an example to exploit the determinants of cross-border ecommerce trade including the export and the import, respectively. The panel data from the year of 1998 to 2016 will be employed to estimate the relationship between cross-border e-commerce trade and relevant variables under the dynamic ordinary least squares and the error correction model. The findings of this paper show that there is a long-run relationship between cross-border e-commerce trade and relevant variables. Generally speaking, the GDP(+) and real exchange rate(-export & +import) have an effect on cross-border e-commerce trade. However, the population (+) and the terms of trade (-) only have an effect on cross-border e-commerce import. The empirical evidences show that the GDP and the real exchange rate always affect the development of cross-border e-commerce trade. Therefore, all ASEAN countries should try their best to develop the economic growth and focus on the exchange rate regime so as to meet the need of crossborder e-commerce trade development.

With the increasingly mature development of China's Internet technology, China's e-commerce has developed, which has given a huge boost to China's economic growth. In recent years, mobile payment has become a bright spot in China's economy, promoting the further development of e-commerce. On the one hand, various e-commerce sales and service platforms in China have greatly developed and with Alibaba as the representative, the annual turnover of e-commerce sales platform has increased year by year. On the other hand, with the implementation of the "one belt and one way" policy, cross-border electricity providers have promoted China's trade with the world and become China's new economic growth point. This paper analyzes the current situation of e-commerce in China and puts forward Suggestions for the further development of e-commerce in China.

Shipping containers promote rapid development of the transport industry, and generate economic and social value. However, problems often occur due to imbalance in dynamics between exports and imports, container relocation, traffic congestion, and general shipping network designs. So, efficient and cost-effective cargo management has become crucial for the Jeju Island – Mokpo container route (JMCR) in South Korea. Dozens of recent studies reveal that collapsible or foldable containers, may become an innovative solution to problematic issues within the shipping industry. The major purpose of this study is to discuss conditions required for successful commercial application of foldable containers, based on a cost-benefit analysis precisely for the JMCR. Findings reveal usage of foldable containers can be cost-effective, if containers make a “FULL<->EMPTY” route. To the contrary, a “FULL<->FULL” route can drastically increase expenditure. However, economic analysis of actual figures for 2010-2015 indicate that benefits for Mokpo – Jeju direction are significant to cover losses on the Jeju – Mokpo line. Seasonal patterns and mixing percentages of foldable and standard containers on the route, may also produce cost-effective solutions. Sensitivity analysis reveals that benefits from using foldable containers, depend mostly on empty container shares in addition to various extra costs.

Researches based on the pattern of planned behavior holds that the three variables of entrepreneurial attitudes, subjective norms, and perceived behavior control influence each other and influence entrepreneurial intentions respectively. However, there are also different, even conflicting research conclusions that continue to emerge. Researches based on the pattern of alertness, believe that profit opportunities and individuals’ pursuit to truth are the driving forces. Many scholars have demonstrated the impact of individual entrepreneurial alertness on entrepreneurial intentions. However, as an exogenous causal agent, profit opportunities have a logical problem: if there are no other assumptions, the mere existence of opportunities does not adequately explain entrepreneurial alertness. To address this gap, this study considered samples from mainland China, where entrepreneurial activities are very active currently, to test the role that entrepreneurial alertness is assumed to play in the planned behavior model. The results show that the three dimensions of alertness, individually partly intermediate the influence of entrepreneurial attitude on entrepreneurial intention, the influence of subjective norms on entrepreneurial intention, and that of perceived behavior control on entrepreneurial intention. This article studied the production of entrepreneurial intention by integrating the two patterns of planned behavior and alertness through empirical analysis, and opened up a new field for subsequent research on entrepreneurial intention.

The study examines the magnitude of economic spillover and the impact of foreign direct investment (FDI) inflows on the efficiency of the bank industry in China. This study employs unit root tests, cointegration tests and cointegrating regression analysis, including fully modified ordinary least squares (FMOLS), canonical cointegrating regression (CCR) and dynamic OLS (DOLS) to test the proposed hypotheses. The sample is restricted to the period of time in which monthly data is available and comparable among variables for the period from January 2002 to October 2013 (142 observations). All of the time series data was collected and retrieved from the People’s Bank of China, China Monthly Statistics from the National Bureau of Statistics of China, and International Financial Statistics database from International Monetary Fund. The results of the Johansen cointegration test suggest that there is a long-run equilibrium relationship between FDI inflows, foreign exchange rate and banks performance in China. The results of cointegrating regression analysis using FMOLS, CCR and DOLS suggest that M2 supply and FDI inflows are significant at the 0.01 level. The results confirm that FDI inflows in the banking sector are positively related to the increase of banks productivity and performance and short-term loans in China. However, the results suggest that Chinese Yuan currency exchange rate to U.S. dollar is not significant in the banking and financial industry of China.

Purpose - This research addresses the question of how an international retailer like Costco can succeed in a foreign mature market and satisfy the local consumers. Our study aims to promote our understanding of how foreign retailers influence local consumers in a mature market with differentiated business models. Research design, data, and methodology – Our study uses company publications, secondary sources of information and the results of a questionnaire survey consisting of 106 participants. Consumer responses were solicited through a questionnaire survey conducted in the city of Kobe in December of 2013. Results - Product differentiation from local retailers in a mature market like Japan gave Costco a competitive edge. Compared with local supermarkets, Costco was preferred by Japanese consumers for its variety of goods that it carries, as well as in-store promotion large package of selling units, in-store amenities, and customer services. Conclusions - First, a theoretical framework is proposed in this study that can aid in developing localization strategies in a mature market such as Japan. Second, it reveals that an international retailer can succeed in a foreign market by stimulating local consumers to change their purchasing behavior, without having to alter the prevailing format of operation.

Purpose –This paper is to study the process of the negative effects of Sino-foreign joint ventures, hoping to find out an effective path in which local enterprises can avoid the risks in the utilization of foreign capital, and ultimately achieve independent innovation. Research design, data, and methodology –The outflow of assets, the loss of local brands and the "technology hollowing-out" problem brought by joint ventures is becoming more and more serious. Based on conflict analysis graph model, this paper takes Yuhuan joint venture as an example to identify the conflict problem in Sino-foreign joint ventures. Results –The results of the stability analysis show that establishing joint venture cannot really realize the introduction of technology because the technology is fully controlled by the foreign part. So when introducing foreign capital, local enterprises should participate in R&D and master the initiative. Conclusions – Local enterprises should pay attention to patent containment and technology blockade of multinationals. Domestic enterprises should try to protect state-owned assets and local brands in Sino-foreign joint ventures. Independent innovation is the most effective strategy for the development of enterprises in China.

Stachys affinis tubers are known for its high content of stachyose and eaten as an edible vegetable. In this work, we assessed on the anti-inflammatory and anti-proliferation activity of a various type of extracts derived from S. affinis tubers. The n-hexane and dichloromethane fractions were showed the high cytotoxicity on the cell lines including RAW264.7 macrophages, HEK293 human kidney cell, A549 human lung cancer cell, KB human oral cancer cell, and a PC-3 human prostate cancer cell. N-butanol and water fractions were not exhibited cytotoxicity on the tested cancer cells, limited in anti-inflammatory and anti-cancer activities. Nevertheless, the ethyl acetate fraction showed little harm to RAW264.7 cells but inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) significantly. In addition, it arrests the cell growth in A549, KB, and PC-3 cell while little cytotoxicity on HEK293 cells. Consequently, these results supported that the ethyl acetate fraction of S. affinis tubers could be a potential anti-inflammatory and anti-cancer ingredient.

Ascidian embryos have become an important model for embryological studies, offering a simple example for mechanisms of cytoplasmic components segregation. It is a well-known example that segregation of mitochondria into muscle lineage cells occurs during ascidian embryogenesis. It is, however, still unclear what signaling and molecular event control polarized distribution of mitochondria in the early ascidian embryonic development. To obtain molecular markers for studying mechanisms involved in polarized distribution of mitochondria, we have produced monoclonal antibodies, Mito-1, Mito-2 and Mito-3, that specifically recognize mitochondria-rich cytoplasm in all cells of the ascidian Halocynthia roretzi embryos. These antibodies stained cytoplasm like a mesh structure in epidermis cells, except for nuclei, at the early tailbud stage. Similar pattern was observed in vital staining of mitochondria with DiOC2, a fluorescent probe of mitochondria. These antibodies showed that mitochondria were distributed evenly in the animal hemisphere blastomeres at cleavage stages, whereas did not in the vegetal hemisphere blastomeres. Mitochondria were transferred more into cells of the marginal zone, such as muscle and nerve cord lineage cells, than into cells of the central zone, such as mesenchyme, notochord and endoderm lineage, in the vegetal hemisphere. Therefore, it is suggested that these antibodies may be useful as markers for analysing mechanisms involved in polarized distribution of mitochondria during ascidian embryogenesis.

Asymmetric cell divisions play crucial roles during ascidian embryogenesis. In these processes, an FGF signaling is an essential inductive signal for establishing cell fate polarization, such as mesenchyme and notochord. It was well reported that the FGF signaling cascade is composed of FGF, FGF receptor, Ras, MEK, Erk and Ets. However, mechanisms of communication between the FGF and other signaling pathways and of integrated regulation of signaling pathways have remained largely unknown. In this study, we isolated HrS6K, a homologue of the S6K gene that belongs to the S6-H1 kinase of the ribosomal S6 kinase family, and HrNck1, a homologue of Nck1 gene that encodes an adaptor protein containing Src homology 2 and 3 domains, from the ascidian Halocynthia roretzi, to elucidate the mechanisms. Zygotic expression of HrS6K was initiated as early as the 16-cell stage. In the 64-cell stage embryos, expression of HrS6K was seen in mesenchyme precursor cells. The signal was detected in dorsal midline cells and mesenchyme clusters of the early tailbud embryos, and then down-regulated by the late tailbud stage. In adults, HrS6K mRNA was highly detected in muscle and stomach by QPCR method. On the other hand, HrNck1 transcripts are detected maternally. Zygotic HrNck1 mRNA was strongly expressed in mesenchyme clusters of the neurula, and in tail tip cells of the early tailbud embryos. These results suggest that HrS6K and HrNck1 are involved in formation of mesenchyme cells, which are specified by the FGF signaling.

To create urban landscapes that take human emotion into consideration, the present study verified the psychological effects of artificial waterscape facilities on users, as these facilities significantly impact their psychological comfort. Data was collected using the SD scales and POMS of 60 male and 60 female participants after they watched a video of four waterscape facilities. Participants deemed the video clip of a fountain waterscape to be artificial and linked it with changeable images that increased their vigor. The video clip of waterfall stimulated various impressions (e.g., vital, liked, active, etc.) and changed participant’ mood states by increasing their vigor and decreasing fatigue. The video clip of the pond yielded familiar impressions, produced less free images, and decreased tension among participants. Finally, the video clip of the stream stimulated quiet and comfortable images as well as reduced negative feelings of anger, confusion, and depression among participants. Furthermore, males experienced more positive effects than females, regardless of the type of waterscape facility. The study findings indicate that the four different waterscape facilities influenced participants’ mood states. Additionally, the psychological effects differed by gender. The data suggest that landscape planners need to carefully consider their choice of waterscape facility when designing green spaces to ensure that the space is psychologically comforting to users.

Cranioplasty following decompressive craniectomy is regarded as a relatively simple and safe procedure to protect the brain and improve neurological function. However, some serious complications, such as infection, hematoma, bone resorption, seizure, and epidural or subdural fluid collection may develop after cranioplasty. Among these complications, epidural fluid collection after cranioplasty is not well described and with limited reports on the same. Here, we report recurrent epidural fluid collection after cranioplasty, eventually necessitating the removal of the bone flap. This study discusses the possible pathological mechanisms of this undesirable complication with a review of the literature.

The anti-diabetic effect of Cirsium setidens water extract and the combinations with Bletilla striata, Cymbidium kanran, and Sparganium stoloniferum Buch.-Ham. ethanolic extracts had been studied. The combination of four extracts (3:1:1:1) showed larger anti-diabetic activity in vitro and in vivo. It is notable that the single water extract from C. setidens exhibited more effective anti-diabetic effect than most of the combinations. We also investigated whether fermentation process was promoted the anti-diabetic activity. The data suggested the fermentation product of combination of four extracts (3:1:1:1) exhibited the strongest activity both in vitro and in vivo, which was higher than the non-fermented group. The result indicated the fermentation and the appropriate combination of extracts enhanced the anti-diabetes activity.

The authors calculate the long-term predictability of GDP, domestic demand, investment, and net exports for Guangdong province, P.R. China from 2000 to 2013. A vector autoregressive (VAR) model with quarterly data for this period is first co-integrated then the Granger causality test is applied to empirically assess the relationships among gross domestic product (GDP), consumption, investment, and net exports. There is a strong causality effect between investment and net exports in Guangdong province. However, the variance decomposition results indicate that exports respond to foreign shocks rather than domestic ones, making their impact on the Guangdong economy to predict. Results show the stimulating effect of domestic demand on GDP is larger than the stimulating effect of net exports and much larger than even the stimulating effect of investment. The analysis suggests that there are dynamic influences with various levels of persistence between GDP, consumption, investment, and net exports. Macroeconomic policy adjustments are urgently required to expand domestic demand and thereby stimulate economic growth in Guangdong province.

The follicle loss of transplanted ovarian tissue (OT) is caused by ischemia and slow revascularization. To shorten the ischemic period and promote angiogenesis, some angiogenic factors have been treated for transplanted tissues. Angiopoietin-2 (ANG-2) is one of the major angiogenic factors and has been reported to promote blood vessels and increase vascular permeability in the ischemic and/or hypoxic environment. So, this study was designed to assess the impact of ANG-2 on follicle integrity and revascularization of mouse OT grafts. The 5-week-old B6D2F1 female mice were divided into 3 groups (a control and 2 ANG-2 groups) followed by ovary collection and vitrification. After warming, the ovaries were autotransplanted into kidney capsules with/without ANG-2 injection (50 or 500 ng/kg), and then killed at day(D)2, 7, 21 and 42 after transplantation. Total 2,437 follicles in OT grafts were assessed for the follicular density, integrity and classification by H&E staining. Apoptosis, revascularization, and serum FSH levels were evaluated by TUNEL assay, CD31 immunohistochemistry, and ELISA respectively. All the ANG-2 groups showed remarkable increase of morphologically intact follicle ratio across all the grafting duration except D21 (no statistical difference). The numbers of CD31(+) vessels (the sum of 3 fields at ×400 magnification) were significantly increased in both ANG-2 groups compared with the control group at all the grafting duration. Especially at D42, the 500ng ANG-2 group showed significantly more vessels than the 50 ng ANG-2 group as well as the control group. However the mean follicle numbers of grafts, apoptosis ratio and serum FSH levels showed no significant difference among the groups. In this study, remarkably well preserved follicles and larger amount of vessels were appeared in ANG-2 treated groups. So we thought that ANG-2 treatment is effective for OT transplantation and improve transplantation outcomes.

Fungal blast caused Magnaporthe oryzae, bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo), bacterial streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) are devastating diseases of rice worldwide. Application of host resistance to these pathogens is the most economical and environment-friendly approach to solve this problem. Some major disease resistance (MR) genes controlling qualitative resistance and quantitative trait loci (QTLs) controlling quantitative resistance are valuable sources for broad-spectrum and durable disease resistance. We have characterized a number of rice MR genes and resistance QTL genes that confer a broad-spectrum or durable resistance to M. oryzae, Xoo, and Xoc. How to efficiently use these genes for rice improvement will be discussed.

Early pregnancy loss in humans, which often occurs due to defects that occur before, during or immediately after implantation, is a worldwide social and economic concern. For successful implantation to occur in the receptive uterus, the blastocyst must also attain implantation competency. The first evidence that the state of activity of the blastocyst determines the “window” of implantation in the receptive uterus was derived from reciprocal blastocyst transfer experiments in a delayed implantation mouse model. This model is a powerful approach to define the molecular signaling components that direct blastocyst activation or dormancy. Nearly 100 mammals in seven different orders undergo delayed implantation, but the underlying mechanism remains largely unknown. There is evidence that catecholestrogens produced from primary estrogens in the uterus activate blastocysts. Another lipid signaling molecule that targets blastocysts is an endocannabinoid anandamide, which activates G-protein coupled cannabinoid receptors CB1 and CB2. Expression of CB1 in the Tr, and uterine synthesis of anandamide, suggest that endocannabinoid signaling is critical to implantation in mice. Levels of uterine anandamide and blastocyst CB1 are coordinately downregulated with the attainment of uterine receptivity and blastocyst activation, respectively, in contrast to their elevated levels in the nonreceptive uterus and dormant blastocysts. Anandamide regulates blastocyst function by differentially modulating MAPK signaling and Ca2+channelactivityviaCB1. Using delayed implantation model, a global gene expression study showed that these two different physiological states of the blastocyst are molecularly distinguishable. The main functional categories of altered genes include cell cycle, cell signaling and energy metabolic pathways. This study also showed an upregulated expression of heparin-binding EGF-like growth factor (HB-EGF) in activated blastocysts and is complementary to earlier reports of upregulated expression of its receptor ErbB1 and ErbB4 in similar blastocysts. Recently, we demonstrated that silencing of Wnt-beta-catenin signaling in mice does not adversely affect the development of preimplantation embryos to blastocysts and uterine preparation for receptivity, but, remarkably, blocks blastocyst competency to implantation. A coordinated activation of canonical Wnt-beta-catenin signaling with Cox-2-PPARd signaling pathway ensures blastocyst competency to implantation. These findings constitute novel evidence that Wnt signaling is at least one pathway that determines blastocyst competency for implantation. More insight into the molecular basis of blastocyst competency for implantation might help to improve pregnancy rates in human IVF programs.