We determined the complete mitochondrial genome of the yellow-spotted long horned beetle, Psacothea hilaris (Coleoptera: Cerambycidae) that is an endangered insect species in Korea. The genome was sequenced from four overlapping fragments: two short fragments and two long fragments. The 15,857-bp long P. hilaris mitochondrial genome has the gene content typical of animal mitochondrial genome: 13 protein-coding genes, 22 tRNA genes, 2 ribosomal genes, and one non-coding A+T-rich region. The gene arrangement of the molecule is identical to the most common type found among insect mitochondrial gene arrangement that is regarded as ancestral for insects. Like several other coleopteran species the P. hilaris COI gene has typical mitochondrial start codon ATT. The 1,190-bp long A+T-rich region contains 57-bp long seven identical repeat sequences and at least seven stem-and-loop structures, composed of stems with perfect matches and loops with variable size. All P. hilaris tRNAs can be folded into the typical clover-leaf structure, with the exception of tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. After more genomic and phylogenetic analyses are performed, further detailed information will be available.

In this study, we determined the complete mitochondrial genome of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae), from four overlapping fragments. The 15,592-bp long C. fulgidissima mitogenome exhibits a gene arrangement and content identical to the most common type in insects. The start codon of the C. fulgidissima COI gene is unusual, in that no typical ATN codon is available. The 875-bp A+T-rich region is the shortest among the coleopteran mitogenomes that have thus far been sequenced in their entirety. The most unusual feature of the genome is the presence of three tRNA-like sequences within the A+T-rich region: two tRNALeu(UUR)-like sequences and one tRNAAsnlike sequence. These sequence stretches evidence the proper anticodon sequence and the potential to form secondary structures, but also harbor many mismatches in the stems. Phylogenetic analysis using a concatenation of 13 amino acid sequences of protein-coding genes among the available sequenced species of coleopteran superfamilies (Buprestoidea and Elateroidea belonging to the infraorder Elateriformnia, and Chrysomeloidea and Tenebrioroidea belonging to the infraorder Cucujoiformia) by Bayesian inference, maximum-parsimony analyses, and maximum-likelihood analysis unexpectedly revealed a lack of support for monophyletic Elateriformia.

The 15,389-bp long complete mitochondrial genome (mitogenome) of the redspotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, but differs from the most common type, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. No typical start codon of the P. bremeri COI gene is available. Instead, a tetranucleotide, TTAG, which is found at the beginning context of all sequenced lepidopteran insects was tentatively designated as the start codon for P. bremeri COI gene. All protein-coding genes (PCGs), but COII (T) have complete termination codon TAA or TAG. One of the most unusual feature of the P. bremeri mitochondrial genome is the presence of two tRNA-like structures, such as the tRNATrp-like sequence and tRNALeu (UUR)-like sequence with proper anticodon and clover-leaf structures in the 514-bp long A+T-rich region. Furthermore, the A+T-rich region possesses three sequences that have the potential to form stem-and-loop structures, flanked by the conserved sequences, "TA(A)TA" at the 5’ end and "G(A)nT’ at the 3’ end. After more genomic and phylogenetic analyses are performed, further detailed information will be available.

The completely sequenced mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys, is a circular molecule of 16,518 bp with a total A+T content of 76.4%. Nucleotide composition and codon usage of this genome are near the means observed in other 12 hemipteran mitochondrial genomes; however, the initiation codon for CO1 gene appears to be TTG, dissimilar to what has been seen in the 12 mitochondrial genomes. In this genome, the A+T rich region between srRNA and tRNAIle gene includes two extensive repeat regions, in which each region includes 4 and 12 tandem repeats of a 73 bp sequence, respectively. The gene content, order, and structure of the H. halys mitochondrial genome are consistent with that of Triatoma dimidiata, belong to the same suborder Heteroptera, but different from two suborders, Auchenorrhynca and Sternorrhyncha, including various gene rearrangements. Analyzing phylogenetic relationship and comparing gene order and content of the 13 hemipteran mitochondrial genomes of three suborders, Heteroptera, Auchenorrhynca, and Sternorrhyncha, supported the morphology-based current hypothesis that both Auchenorrhynca and Sternorrhyncha are a monophyletic group.

We determined the complete mitochondrial genome (mitogenome) of the Japanese Oak Silkmoth, Antheraea yamamai (Lepidoptera: Saturniidae) from two overlapping fragments and subsequent shotgun sequencing. The 15,601-bp long A. yamamai mitogenome contains gene arrangement and content identical to the most common arrangement found in lepidopteran insects. Most individual A. yamamai mitochondrial (mt) genes were well within the range found in the respective genes of other insects, except for small ribosomal RNA (1,037 bp). The 336-bp A+T-rich region is relatively smaller than that of other lepidopteran insects. The region is interesting in that it contains tRNA-like structures as found in the A+T-rich regions of other insect mitogenomes. The start codon of A. yamamai COI gene is unusual in that no typical one (ATN) is available. Three of the 13 protein-coding genes have incomplete termination codon T or TA. All tRNA formed stable stem-and-loop structure, except for tRNASer(AGN), the DHU arm of which formed a simple loop as seen in many other metazoan mt tRNASer(AGN).

We determined the complete mitogenome of the oriental mayfly, Ephemera orientalis (Ephemeroptera: Ephemeridae) and the dragonfly Davidius lunatus (Odonata: Gomphidae). The 16,463-bp long E. orientalis and the 15,912 bp long D. lunatus mitogenome contains gene arrangement and content identical to the most common type found in a diverse insect order. Most individual E. orientalis and D. lunatus mt genes were well within the size found in the respective genes of other insects. The initiation codon for the D. lunatus COI gene was typical as ATA, whereas no typical start codon was found in the start region of E. orientalis COI gene. The A+T-rich regions of both mitogenomes have a few unusual feature. The A+T-rich region of E. orientalis contains a tandem repeat composed of two identical copies of 55 bp long, whereas that of D. lunatus contains a tandem repeat composed of duplicated identical 261-bp copies and one partial copy of the repeat. Also, the A+T-rich region of E. orientalis contains a single sequence and that of D. lunatus contains nine sequences, along with the tandem triplicate sequences, that has the potential to form stem-and-loop structures, flanked by the conserved sequences, “TA(A)TA” at the 5’ end and “G(A)nT’ at the 3’ end. Furthermore, the A+T-rich region of D. lunatus contains two tRNA-like structures, tRNALeu(UUR)-like sequence and tRNATyr-like sequence that have proper anticodon TAA and clover-leaf structure that were previously found in the hymenopteran insects.

We determined the complete mitochondrial genome (mitogenome) of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae) from two overlapping fragments and subsequent sub fragments. The 15,592-bp long C. fulgidissima mitogenome contains gene arrangement and content identical to the most common arrangement found in insects. Most individual C. fulgidissima mitochondrial (mt) genes were well within the range found in the respective genes of other insects. The 875-bp A+T-rich region is shortest among the coleopteran mitogenomes sequenced in their entirety. The region is interesting in that it contains several stem-and-loop structures and tRNA-like structure found in the A+T-rich regions of other insect mitogenomes. As seen in other insect motogenomes the start codon of C. fulgidissima COI gene also is unusual because no typical start codon is available. Three of the 13 protein-coding genes have incomplete termination codon T or TA. All tRNA formed stable stem-and-loop structure, except for tRNASer(AGN), the DHU arm of which formed a simple loop as seen in many other metazoan mt tRNASer(AGN).

In this study, the paint, which has very close relationship to the of indoor air quality, was examined for the air pollution leveling test, and using the various passive sampler, the test was evaluated on the regard of TWA(Time Weighted Average). This study is to examine how the air pollution levels differ between the regular paint that is used for public and the new developed environment friendly paint. The consecutive experiments were performed in the small chamber test to investigate the VOCs and formaldehyde, ammonia emissions and characteristics from paint which are commonly used as the complete material of building. In conclustion, the natural paint showed the highest emission of VOCs among the selected paints, and water paint showed the highest contents of ammonia. Formaldehyde showed N.D(Not Detected) in most of tests and found a small amount in vinyl paint however, it was not as bad as to fail the certify level of building materials. It is recommended to constantly develop the chamber system and it will be very much possible to be used for the estimation of indoor air pollutants from the various paints and other complete materials.

A three-dimensional (3-D) method of analysis is presented for determining the free vibration frequencies and mode shapes of solid paraboloidal and complete (that is, without a top opening) paraboloidal shells of revolution with variable wall thickness. Unlike conventional shell theories, which are mathematically two-dimensional (2-D), the present method is based upon the 3-D dynamic equations of elasticity. The ends of the shell may be free or may be subjected to any degree of constraint. Displacement components ur, uθ, and uz in the radial, circumferential, and axial directions, respectively, are taken to be sinusoidal in time, periodic in θ, and algebraic polynomials in the r and z directions. Potential (strain) and kinetic energies of the paraboloidal shells of revolution are formulated, and the Ritz method is used to solve the eigenvalue problem, thus yielding upper bound values of the frequencies by minimizing the frequencies. As the degree of the polynomials is increased, frequencies converge to the exact values. Convergence to four digit exactitude is demonstrated for the first five frequencies of the complete, shallow and deep paraboloidal shells of revolution with variable thickness. Numerical results are presented for a variety of paraboloidal shells having uniform or variable thickness, and being either shallow or deep. Frequencies for five solid paraboloids of different depth are also given. Comparisons are made between the frequencies from the present 3-D Ritz method and a 2-D thin shell theory.

Objectives: To develop normative data for normal young adults, to interpret patients’ CMDT score objectively and select the employees who require manual dexterity. Methods: Participants were sixty normal subjects (thirty males and thirty females) between ages of 19 and 26. Averaged performance duration of normal subjects on the CMDT was recorded and Pearson Correlation Coefficients between subtests of CMDT were analysed. Results: Women’s performance duration was shorter than that of men’s, but the percentile scores were different according to the percentile. Pearson Correlation Coefficients were significantly different between the subtests, but not in the results of comparing ‘displacing’ and ‘one hand turning and placing’. Conclusions: These data will assist occupational therapists and others to interpret patients’ CMDT scores objectively.

본 증례연구는 복합손상을 가진 척수손상환자의 재활치료과정을 소개하여 유사한 사례의 치료에 도움이 되고자 하는 것이다. 증례연구의 대상자인 26세의 남자환자는 흉수 4번 완전손상과 사고 당시 전기화상에 의한 좌측하박 절단과 우측손의 정중신경이 마비되었다. 치료초기에는 일상생활동작 검사에서 MBI (Modified Barthel Index) 점수가 22점으로 독립적으로 가능한 것은 거의 없었고, 기능적으로도 모든 도움이 필요한 상태였으나 재활치료결과 독립적

비옥도(肥沃度)가 상이(相異)한 토양(土壤)에서 잎담배를 재배(栽培)할 경우 단위면적당(單位面積當) 최대(最大) 수납대금(收納代金)을 확보할 있는 시비량(施肥量)을 결정(決定)하기 위하여 비옥도(肥沃度)에 따라 상(上), 중(中), 하(下)로 구분(區分)하여 시비반응(施肥反應)을 조사(調査)하고 그에 따른 수량(收量)과 품질(品質)을 조사(調査)하였다. 상급지토양(上級地土壤)은 유효인산(有效燐酸), 유기물(有機物) 및 무기태질소(無機態窒素)의 함량(含量)이 높았다. 잎담배 중(中)의 전질소(全窒素), nicotine 및 reducing suger의 함량(含量)은 후엽(厚葉) 3등(等)이 박엽(薄葉) 3등(等)에 비하여 높은 것으로 나타났다. 연초용(煙草用) 복합비료(複合肥料)의 시용량(施用量)을 증가(增加)함에 따라 전질소(全窒素) 및 nicotine의 함량(含量)은 증가(增加)하였고, reducing sugar의 함량(含量)은 감소(減少)하였다. 잎담배의 수량(收量)은 상급지토양(上級地土壤)에서 75kg/10a, 중급지(中級地)와 하급지토양(下級地土壤)에서 100kg/10a이 시비량(施肥量)일 경우 가장 양호(良好)하였다. 잎담배의 수량(收量), 품질(品質) 및 대금(代金)으로 볼 때 적정시비량(適定施肥量)의 한계(限界)는 상급지(上級地)는 50~75kg/10a, 중급지(中級地)와 하급지(下級地)는 100kg/10a이하(以下) 이었다.