본 연구는 필리핀의 생물자원 및 농업에 이용되어지는 식물자원과 그 근연종의 다양성 및 관리 현황에 대한 최근 정보를 공유함으로써, 유용 유전자원의 확보와 유전자원 관련 국제기술 협력사업의 효율을 높이기 위한 기초자료를 제공코자 하며, 수행한 결과는 다음과 같다. 1. 필리핀은 7,107개의 크고 작은 섬들로 구성되어 있는 300,000 km2의 면적을 보유하고 있으며, 식물보유종으로 세계 5위를 차지할 정도로 다양성이 높은 국가이다. 2. 전 국토의 32% 정도의 면적이 경작지로 이용되고 있으며, 주요작물로는 벼, 옥수수, 코코넛, 사탕수수, 바나나, 마닐라삼, 망고 등이고, 벼멸구 내성을 지닌 O. offininalis 등 주요작물의 야생종 및 야생근연종들이 다수 분포하고 있다. 3. 필리핀 농업자원의 현지내 보존은 농가 보존형태이며, 야생종 보호를 위해 NIPAS 65개 구역을 지정하여 운영하고 있으나 작물연계한 생물다양성이나 생태시스템 관리 등 종합적인 프로그램은 부족하다. 4. 총 173,205점의 자원들이 현지외 보존되고 있으며, 이중형태특성평가는 40%, 생화학평가는 7%, 분자학적 평가는 3%, 병충해나 생산성 등 평가는 60%가량 수행되었다. 5. 식물자원의 보존과 관리를 위한 정부연구기관 및 네트워크가 설립되어 있으며, 식물유전자원에 관한 연구는 DA, DENR, 그리고 DOST 등의 국가적 조직들과 협력하여 수행하고 있다. 6. 공화국법령 8435 등 식물자원의 보존과 이용을 위한 상세한 내용의 필리핀 국내법이 마련되어 있다.

The objective of this study was to determine the best performing switchgrass (Panicumvirgatum L.) cultivar with three different seeding dates as a bioenergy source in Republic of Korea. Split-plot in time with three replications was performed and three switchgrass cultivars, Carthage (CT), Cave-in-Rock (CIR), and Forestburg (FB) were used in this experiment from 2009 to 2012. Plots were seeded on April 23, May 4, and May 13, 2009 and were harvested once in November each year. No fertilizer was applied to the field for the first year; however, in second and third years (June 2010 and May 2011, respectively), N, P2O5 and K2O fertilizers were applied in 67,45 and 90 kg ha-1, respectively. Soil pH (5.9) and climate condition including temperature (10.4∼17.5℃) and precipitation (89.4~109.8 mm month-1) were suitable for switchgrass cultivation. Total dry matter yields were higher in CT and CIR compared to FB and were 16.9, 15.9, and 4.5 ton ha-1, for CT, CIR, and FB, respectively (p<0.0001). The samples were analyzed for dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude fiber (CF), ether extract (EE), and ash. No significant differences in energy content (p = 0.96) and chemical composition among cultivars (p>0.05) were found. Seeding dates did not affect DM yield (ton ha-1), chemical composition and energy content significantly (p>0.05). Significant difference was observed for heights among CT, CIR, and FB (177.59, 169.98, and 94.89 cm, respectively, p = 0.0002). In conclusion, based on soil characteristics and climate condition in Korea compared to other countries, switchgrass can be cultivated successfully. Considering dry matter yield and energy content of these three cultivars of switchgrass CT and CIR adapted better to climate in Middle Eastern of Republic of Korea than Forestburg for bioenergy purpose.

Antitrust deals with the competitive consequences of conduct by firms in the market. Marketing places emphasis on understanding how firms compete from individual perspective; by studying the thinking and motivations of managers and purchase preferences of consumers. As an academic discipline marketing aims to describe and predict the performance of companies engaged in exchange to reach organizational goals (Gundlach, Phillips, & Desrochers, 2002). For antitrust, the complementary nature of marketing's constitutes in providing a basis for understanding the nature of competitive conduct and the welfare of consumers. The influence of antitrust on marketing strategy raises many concerns. There is a strand of literature investigating how the antitrust law perceives different marketing activities. Many common marketing practices are coming under greater scrutiny from regulators, antitrust lawyers and scholars. It is essential for companies to understand how that will affect competition. When considering marketing issues such as distribution policy, product line extension, enhancing the company’s positive image, they may not realize the increasing likelihood of violating antitrust laws (Bush & Gelb, 2005). Brodley (1982) analyzes how joint ventures may threaten competition by falsifying competitive incentives among joint venture participants. He describes various incentive-modifying remedies that mitigate anticompetitive risks, and then applies this presumptive approach to various types of joint ventures. Some papers analyze how antimonopoly decisions can influence management of transaction. Joskow (2002) shows the importance of the application of transaction cost economics (TCE) to antitrust legal rules and antitrust remedies specification because it may result in different legal rules comparing to cases ignoring TCE ideas. He emphasizes that antitrust legal rules must be sensitive to the information and analytical capabilities of institutions in the market, the characteristics of potential anticompetitive behavior, market structures etc. Not only the development of marketing practices challenges antitrust enforcement. Antitrust policy can impose significant and long-lasting restrictions on marketing policies of companies, including pricing decisions. One relevant for Russia example is pricing antitrust remedies under the merger deals clearance or investigations on the abuse of dominance. In order to prevent abuse in a form of excessive price antitrust authorities set ‘soft price cap’ on the domestic market price using different benchmarks including quotes in the world commodity markets, price of export contracts, price of export contracts net of transport cost and custom duties, best (lowest) price of export contract etc. This type of remedies is already applied for chemicals, electric steel, cocking coal, aluminum and other commodities. Reform of tariff regulation in Russia takes this path also: from the next year price cap for natural gas for industrial customers will be set at the level of contract price of the largest supplier Gazprom net of transport cost. We argue that this type of remedies being imposed in order to protect customers of dominant company can diminish incentives to compete, along with the restricting incentives for an efficiency-improvement. This paper investigates how soft price regulation affects companies` behavior. In many regulated industries over the world price cap as a method of price regulation replaces cost-plus pricing. It is a kind of incentive regulation introduced in order to enhance productive efficiency by strengthening sellers’ incentives for cost reduction (Laffont, 1993; Cabral & Riordan, 1989) as well as incentives for more efficient pricing. A price-cap regulation might be suggested to address the market by making it extremely difficult for the industry to use price as a marketing strategy and by reducing the available sources the industry has for spending on marketing and lobbying. However pricing under cap is not neutral for competition in the market. We argue that the impact of price cap regulation on market competition depends on the design of cap. More specifically if cap for one (regulated) market depends on the price of the supplier in other (non-regulated) market, there is sub-type of price cap regulation (known in Russian tariff regulation as ‘netback minus’) that enhance incentives to collude in non-regulated market. Traditionally impact of price caps on collusion is analyzed in the framework of focal point problem. Price ceilings might weaken competition serving as collusive focal points for pricing decisions (Schelling, 1960; Scherer & Ross, 1990). According to the Folk Theorem (Tirole, 1988) any individually rational prices can occur as a Nash equilibrium in infinitely repeated games with sufficiently high discount factor. In collusive equilibrium, companies face a coordination problem so that price ceilings may become a focal point on which companies may coordinate. It means that price ceilings may facilitate tacit collusion, increase its stability and lead to higher prices on the market. In addition, price ceilings may become a signal that if firms charge prices below that level than they would not be investigated for collusion by government authorities. Thus, it becomes less risky to maintain collusion at price level that government assess as binding. There is a strand of literature providing the empirical evidence for a collusive focal-point effect of price ceilings in different markets. Knittel and Stango (2003), studying data on state-level price ceilings on credit card charges during the 1980s, find that companies used state-level price ceilings as focal points to sustain tacit collusion. Ma (2007) investigates price ceilings in Taiwan’s flour market and argues that firms set prices above competitive levels during most of the regulation period. Moreover, all flour firms set their prices equal to ceilings. Some studies try to explain the existence of asymmetric retail price adjustments to crude oil price or wholesale price shocks by market power and possible collusion effects (Deltas, 2008; Borenstein, Cameron, & Gilbert, 1997). Sen, Clemente and Jonker (2011) evaluating the effects of price ceiling on retail gasoline prices in Eastern Canada 1999-2007 find the evidence that the enactment of such regulation is significantly correlated with higher prices. A potential explanation is that price ceilings serve as focal points stimulating firms to set higher prices. In contrast, laboratory experiments do not find a collusive focal-point effect of price ceilings. Coursey and Smith (1983) find convergence to the competitive equilibrium prices and no collusive effects of price ceilings in posted-offer markets. Despite in the design of Coursey and Smith (1983) the incentives to collude are small, Engelmann and Normann (2009) also find the evidence against the focal-point hypothesis, who study posted-offer markets with four symmetric sellers but larger incentives for collusion at the price ceiling. Finally, Engelmann and Muller (2011) results again fail to support the focal-point hypothesis. Collusion is as unlikely in markets with a price ceiling as in markets with unconstrained pricing. The possible explanation for different results in the laboratory and in the field is the lack of the focal-point effect in the field. The field results might be driven by other factors. These might include explicit collusion (which might either be triggered by the introduction of the price ceiling or might be easier to keep up in its presence) or other reasons that the empirical studies failed to control for (Engelmann & Muller, 2011). However the design of our research differs from ‘focal point’ approach. It is much closer to the literature on the multimarket contacts (Bernheim & Whinston, 1990; Spagnolo, 1999). Multimarket contacts was also analyzed in the context of multimarket rivalry (Phillips & Mason, 1996) but not from the point of view of cap design as we do. The goal of this paper is to show the mechanism by means of which the establishment of the domestic regulated prices ceiling can promote the stability of collusion and conditions at which it occurs. Intuition of the paper is the following. Under price cap which depends on price in deregulated market deviation from collusion in this market decreases profit in regulated market (in the punishment phase). In the model we think about regulated market as domestic and about completely deregulated market as world market. Under certain conditions the establishment of a price ceiling of domestic market by the principle "price would not excess of the world price" can promote the collusion stability in the world market. We argue that there is an unexpected ratio between the principle of the internal price establishment based on a world indicative and collusion incentives: the difference between the world and internal price to which the regulator aspires is higher, the negative impact of this practice on the competition is higher. Not all the versions of price cap which are based on the world market benchmark provide the same impact on the incentive to collude. Other important variables which reflect transport cost (or more generally all cost of trade). Price cup under model ‘world price plus trade (transport) cost’ provides no impact on the incentives to collude under some level of trade (transport) cost. Price cap under model ‘world price minus trade (transport) cost’ supports the collusion to the greatest extent. This paper represents an interesting focus for the marketing field. The price cap for domestic customer influences companies` incentives to collude in the world markets. Thus, marketing needs to recognize the incentives for cooperative behavior as a strategic marketing tool under soft price cap. Important implication is that pricing remedies imposed on Russian exporting companies will affect prices for the customers of their international competitors.

We aimed to extend the Stimulus–Organism–Response framework. Atmospherics and staff service were evaluated as stimulus impacting attendees’ social/self concept and memory (organism), and further leading to behavioral intention (response). Memory is not only an effective predictor of behavioral intention but also mediates the social/self concept value and behaviors.

The luxury market is surely one of the most affected areas by the counterfeiting phenomenon. The presence of fake goods means very often a financial loss for companies, both in terms of reduced turnover, but also in terms of intangible losses (i.e. brand value reduction). This phenomenon has led companies, in the last years, to ask their New Product Development (NPD) division if and how it was possible to develop unique products, difficult or even impossible to replicate, and how to help customers in the identification of authentic goods versus fake ones. The authors propose a model to support fashion companies for developing anti-counterfeiting solutions since the NPD phases in order to deal with the effect of black and grey market and preserve their brand and products values.

Subjective knowledge (SK thereafter) is defined as what consumers think they know, or their perceived level of knowledge (Brucks, 1985). SK influences what consumers search for, the effort that they put into searching, and the purchasing decision and choice of brands they finally make (Hadar, Sood, & Fox, 2013; Moorman, Diehl, Brinberg, & Kidwell, 2004). The existing literature on consumer knowledge development mainly argues that SK development is associated with consumers’ product related experience, such as product ownership and experience from usage (Alba & Hutchinson, 2000; Park, Mothersbaugh, & Feick, 1994). This study contributes to the literature as follows. We consider a broad market segmentation where the market consists of product owners and non-owners, The literature suggests that these two groups of consumers should have different levels of perceived knowledge due to their different product-specific experience (Park et al., 1994). Our research specifically contends that it is important to distinguish, within the group of non-owners, between those who intend to buy and those who do not intend to buy the product. Thus, this study examines how previous ownership and intention to buy, which is the goal motivation, influence the SK levels of consumers, which in turn impacts on consumers’ heterogeneous preferences for different attributes within a product category. It is contended that the motivation to learn about a product category of non-car owners who have strong intentions to buy influences what this group learns compared to those non-owners who do not intend to buy. More specifically, the research proposes to uncover how learning influences how different groups of consumers perceive their subjective knowledge and how this relationship in turn shapes their preferences, especially when we compare owners and non-owners who have strong intentions of buying a product. Therefore, we develop following research hypotheses: H1: The SK level is positively associated with the product ownership experience. H2: The SK level is positively associated with purchase intention. H3: The relationship between purchase intention and SK level is moderated by the ownership of the product. H4: Preference heterogeneity for different types of brand attributes of the product between owners and non-owners is moderated by the SK level. The empirical context that illustrates the importance of this research is the Chinese car market where the majority of consumers are first time buyers. Our research shows that the SK level is positively associated with both car ownership and purchase intention. Furthermore, the segmentation analysis finds that purchase intention has a significant effect only on non-car owners’ SK level, while the effect on car owners’ SK level is insignificant. An interesting finding is that SK development, in turn, influences consumer’s preferences for different types of brand attributes of a product. We demonstrate the role of SK in forming consumer preference heterogeneity by comparing preferences for a tangible and a non-tangible brand attribute respectively across different consumer segments. We further show how this preference heterogeneity across two consumer segments (car owners versus non-car owners) depends on their SK level. The results of this study can be generalized beyond the specific context of the empirical study of the Chinese car market when marketers are considering new products launch or launching products where the majority of consumers may be unfamiliar with the product.

Motivated by an illustrative case study which assesses the strength of the New Zealand wine brand, we develop a framework that provides a contemporary perspective on Country of Origin (COO) branding. The New Zealand wine industry’s COO branding was initially built around the country image of “green” production with the logo “riches of a clean green land”. This has been superseded by a branding strategy that focuses on developing the collective meaning of the COO brand with the logo “pure discovery”. A collective approach to branding involves broader considerations, where the brand is used to facilitate processes that co-create experience and meaning that reflects collective interest. This entails the alignment of a complex set of industry relationships where value is co-created within a network of stakeholders that contribute to the heritage of New Zealand Wine and its quality positioning as the country-of-origin. Our contribution comes from a wider conceptual understanding of COO to show that “COO matters” when a shared identity and image are integrated to form a collective meaning which co-creates value to fulfil the expectations of a brand’s promises of innovation, authenticity and quality.

This study examines how consumer trust across multiple customer interaction channels, company management policies, and customer previous experience affect service renewal, loyalty. Structural equation modeling of an online survey of 657 US insurance and mobile telecomm consumers confirmed the importance of the three antecedents and that the impact of the three interaction channels differed in the two service contexts.

본 연구는 양념 닭 가슴살의 양념 액에 귤 껍질 추출물(CPE, 2%), 양파 껍질 추출물(OPE, 2%), calcium lactate(2%), 난황 유래 phosvitin(0.1%)과 CPE, OPE 및 calcium lactate 복합처리를 첨가하였을 때 서로 다른 저장온도와 저장기간에 따른 항균 효과 및 품질 변화를 확인하고자 수행되었다. 모든 저장온도와 저장기간에서 CPE, OPE 및 복합 처리군의 총 호기성 세균 수는 대조구에 비해 유의적으로 낮게 확인되었으며, 가장 높은 미생물 생장 억제 효과가 있었던 처리군은 CPE 처리군이었다. 하지만, calcium lactate와 phosvitin 처리군의 미생물의 성장 억제 효과는 나타나지 않았다. pH의 경우, calcium lactate와 OPE 처리군은 모든 저장온도의 저장 0일차에서 대조구에 비해 유의적으로 낮은 값을 나타내었다. OPE 처리군은 모든 저장온도와 저장기간에서 다른 처리구들에 비해 유의적으로 높은 적색도를 나타내었고, CPE와 함께 저장온도에 따른 유의적 차이를 나타내지 않았다. 하지만 이러한 OPE 처리군은 풍미, 맛 및 종합적 기호도에서 다른 처리구들에 비해 유의적으로 낮은 값을 보였으며, calcium lactate와 phosvitin 처리군의 종합적 기호도는 대조구와 유의적 차이가 나타나지 않았다. 결론적으로, 양념 닭가슴살의 CPE, OPE 및 복합처리군에서 저장온도를 달리한 저장기간에 따른 미생물 억제 효과를 확인할 수 있었으나, 관능적 특성을 개선하는 방법의 개발이 필요하다고 판단된다.

Potting media are an integral part of potted plants production system, formulated by a mixture of various raw materials to act as a substrate for plant support and source of nutrition. There is a wide range of potting media available, which are used for quality production of high-value plants worldwide. In this study, quality assessment of Dracaena plants grown in different potting media like: mushroom compost, peat, sand, farmyard manure, coconut coir, and leaf compost in different combinations were carried out. A mixture of sand, mushroom compost, coconut coir, and peat proved to be the best, by promoting root and shoot growth, leaf area, fresh and dry weights of root and shoot, and also improved overall quality of the plant. Media containing garden soil alone or a mixture of sand and leaf compost produced the lowest quality of plants with considerable reduction in most of the growth parameters. Anatomical parameters showed weak relation to media composition as compared to other morphological features. The best medium was made with sand, mushroom compost, coconut coir, peat, that produced plants with thick epidermis and larger pith cells, but thin endodermis and smaller metaxylem vessels in their roots. As moisture availability was sufficient enough in this medium combination, the development of water storing tissue may not contribute significantly. However, epidermis can play a decisive role in protecting roots from external environmental hazards.

The carbon nanotube (CNT) represents one of the most unique inventions in the field of nanotechnology. CNTs have been studied closely over the last two decades by many researchers around the world due to their great potential in different fields. CNTs are rolled graphene with SP2 hybridization. The important aspects of CNTs are their light weight, small size with a high aspect ratio, good tensile strength, and good conducting characteristics, which make them useful as fillers in different materials such as polymers, metallic surfaces and ceramics. CNTs also have potential applications in the field of nanotechnology, nanomedicine, transistors, actuators, sensors, membranes, and capacitors. There are various techniques which can be used for the synthesis of CNTs. These include the arc-discharge method, chemical vaporize deposition (CVD), the laser ablation method, and the sol gel method. CNTs can be single-walled, double-walled and multi-walled. CNTs have unique mechanical, electrical and optical properties, all of which have been extensively studied. The present review is focused on the synthesis, functionalization, properties and applications of CNTs. The toxic effect of CNTs is also presented in a summarized form.

L2 synonyms (and other related words) that are undifferentiated (i.e., are translated as the same word) in learners’ L1 are among the most difficult lexical items for L2learners to grasp. How to help students learn the appropriate use of these words has been a great challenge for L2 teachers. Drawing on recent research in corpus linguistics and corpus-based language teaching, this paper strives to show that corpus analysis can effectively differentiate synonyms and help L2 students successfully learn the appropriate use of the words in a synonym set. The paper begins with a brief overview of research on synonyms and their learning/use. Then using four sets of English synonyms/related words that are not differentiated in Korean, it discusses, with specific examples of learning activities and exercises, how corpus analysis and its results can be used to help learners differentiate these difficult words and grasp their usage patterns. The paper concludes with a summary of both the benefits and challenges of such a teaching approach and some strategies for dealing with the challenges.

Part 2 of the Questionnaire Survey on Marine Safety and VTS in the Philippine Coastal Waters presents the alternatives to risk reduction, modifying the VTS, the qualification and competency of a VTS operator and effectiveness of the services and assistance rendered by VTS. This study finds that majority of respondents chose "Improved education and training of mariners" as the best alternative to risk reduction and the "Strict enforcement of ship safety regulations" as the best alternative based on the respondents' ranks last served onboard and areas of familiarity. In modifying the VTS, the areas with VTS chose to "Improve or upgrade" and for those without, is to "Implement" the system. The best VTS system is the "Port and Approaches" type and the PCG is selected to operate, maintain and supervise the system. As a VTS operator, the necessity of shipboard experience is considered "Essential" and the experiences include "Ship-handling and Communication", and "Management level" experiences. The effectiveness of the assistance and services rendered by VTS are considered "Very Important". Based on these findings, this study recommends improvement of education and training of mariners and users of the waterways including the training of VTS operators manning the VTS centers, strict enforcement of ship safety regulations along the busy and main ports of the country and installation of VTS system with the most appropriate type with the supervision of PCG.

Developmental potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A (TSA) to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embryos. Electrically activated oocytes were treated with 5 nM TSA for different exposure times (0, 1, 2 and 4 hr) and then the activated embryos were cultured for 7 days. The reconstructed embryos were treated with different concentrations of 0, 5, 10 and 25 nM TSA for 1 hr. Also 5 nM TSA was tested with different exposure times of 0, 0.5, 1, 2 and 4 hr. And fetal fibroblast cells were treated with 50 nM TSA for 1, 2 or 4 hr and with 5 nM TSA for 1 hr. Cumulus-free oocytes were enucleated and reconstructed by TSA-treated donor cells and electrically fused and cultured for 6 days. In parthenogenetic activation experiments, 5 nM TSA treatment for 1 hr significantly improved the percentage of blastocyst developmental rates than the other groups. Total cell number of blastocysts in 1 hr group was significantly higher than other groups or control. Similarly, blastocyst developmental rates of porcine NT embryos following 5 nM TSA treatment for 1 hr were highest. And the reconstructed embryos from donor cells treated by 50 nM TSA for 1 hr improved the percentage of blastocyst developmental rates than the control group. In conclusion, TSA treatment could improve the subsequent blastocyst development of porcine parthenogenetic and nuclear transfer embryos.

Although there are a number of reports regarding the toxicity evaluation of inorganic nanoparticles, knowledge on biodegradable nanomaterials, which have always been considered safe, is still limited. For example, the toxicity of chitosan nanoparticles, one of the most widely used drug/gene delivery vehicles, is largely unknown. In this report, we examined the cytotoxic effects of chitosan nanoparticles on mouse embryos at the blastocyst stage and in vivo implantation by embryo transfer. Blastocysts treated with 250 nm chitosan nanoparticles exhibited significantly increased apoptosis and a corresponding decrease in total cell number, which was concentration‐dependent. Moreover, the TUNEL positive signal in the embryos exposed to chitosan nanoparticles showed an increased of the ICM and the implantation success rate was lower than that of their control counterparts. Our results collectively indicate that in vitro exposure to chitosan nanoparticles induces apoptosis and retards implantation development after transfer to host mice. The results collectively show that chitosan nanoparticles have the potential to induce embryo cytotoxicity. Further studies are required to establish effective protection strategies against the cytotoxic effects of these nanoparticles.

The objective of the present study was to investigate the effects of different concentrations of sorbitol supplementation for in vitro maturation medium and in vitro culture medium, on porcine cumulus oocyte complexe(COC) maturation and subsequent developmental capacity after parthenogenetic activation. Porcine COC were cultured for 44 h(0～ 22 h termed MI stage and 22～44 h termed MII stage) in TCM199 without(— ) or with(+) sorbitol (20 μM, 100 μM, 200 μM), and the resultant metaphase II oocytes cultured in PZM-3 for 7 days following activation. Our results showed that supplementation with appropriate concentrations of sorbitol (20 μM) during full term maturation culture(MI+/MII+) significantly(p<0.05) improved blastocyst formation rates and total cell number. When the concentration of sorbitol were increased to 100 μM and 200 μM during maturation culture, the maturation rate of COC were significantly reduced compared with 20 μΜ or control groups. Also blastocyst formation rates significantly(p<0.05) reduced with increasing concentration of sorbitol(200 μM). Supplementation with sorbitol(20 μM, 50 μM, 100 μM) into PZM-3 for in vitro culture significantly(p<0.05) inhibited blastocyst formation compared with control group. However, the blastocyst formation rates start to rise again when 50 μ M sorbitol was used for the first 48 hours and then cultured in PZM-3 without sorbitol. There was no significant difference in cell number between control and sorbitol treated groups. When the activated oocytes were cultured in PZM-3 for 48h and then cultured in PZM-3 with sorbitol, interestingly, the blastocyst formation rate was similar to that of PZM-3 with sorbitol for in vitro culture and significantly lower than control group. These results suggest that addition of low concentrations of sorbitol(20 μM) during oocyte maturation is beneficial for subsequent blastocyst development and improved embryo quality. However, treatment with sorbitol supplementation during in vitro culture medium is negative effect to blastocyst formation.