Hepatoprotective and antioxidant activities of Acer mono and A. okamotoanum were compared after beingextracted by low temperature and high pressure (LTHP) at 20 MPa and 60℃ for 15 minutes. Extraction yield of both A.mono and A. okamotoanum was increased about 40~43% by this extraction process. On scavenging activities, the bark of A.okamotoanum from this extraction process showed the highest activity as about 97%. This value was higher than that fromconventional water extraction and A. mono extracts. Both of A. mono and A. okamotoanum showed high ability on nitritescavenging, but decreasing tendency according to increasing of pH. On SOD-like test, A. okamotoanum had the highestactivity as 46.28% at 1.0㎎/㎖ concentration. A. okamotoanum extracted by LTHP also showed the highest activity as197.38% in adding 1.0㎎/㎖ concentration. Generally, the extracts from low temperature and high pressure extraction pro-cess are higher hepatoprotective and antioxidant activities than that from conventional water extraction. It can concludethat the bark of A. okamotoanum has better biological activities than other parts of A. mo

The low quality fresh ginseng was extracted by water at 80℃ and 240bar for 20min (HPE, High pressureextraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of theextracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at 100℃ in adding themaximum concentration of 1.0㎎/㎖. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhib-ited up to about 86%, in adding 1.0㎎/㎖ of extracts from HPE. This values were 9-12% higher than those from conven-tional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated freshginseng extracts from HPE of 100㎎/㎏/day concentration was very lower than control and other group. The extracts fromHPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibitionactivities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liq-uid chromatogram (HPLC), amount of ginsenoside-Rg2, Rg3, Rh1 and Rh2 on fresh ginseng were increased up to 43-183%by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role ineffectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low qualityfresh ginseng associated with this process has more biologically compound and better anticancer activities than that fromnormal extraction process.