Sucrose and alcohol are rewarding and appetitive. They are occasionally over-consumed and cause addiction. The parabrachial nuclei (PbN) are the second taste relay in the central taste pathway. The nucleus accumbens (NAcc) is an important neural substrate in the reward system. Intake of sucrose or alcohol induces dopamine release in the NAcc. Although alcohol is not classified as a taste stimulus, a substantial number of sucrose-responsive neurons in the PbN respond to stimulation by alcohol on the tongue. In the present study, we investigated whether or not application of 0.5 M sucrose, 10% ethanol (EtOH), mixture of sucrose and EtOH, and double-distilled water (DDW) to the tongue induces c-Fos-like immunoreactivity (cFLI) in the PbN and NAcc. We also examined whether or not the number of cFLI following sucrose/EtOH is comparable to the number of cFLIs following sucrose and EtOH, respectively. Male Sprague-Dwaley rat was anesthetized with a mixture of Zoletil and Rompun while stimulation solution was applied to the anterior tongue. The rat was sacrificed by perfusion, and the fixed brain was sectioned and immunostained. Data from a total of 18 animals were analyzed. The number of cFLI following stimulation with sucrose and/or EtOH was greater than that of DDW in the PbN. Numbers of cFLI following sucrose, EtOH, and sucrose/EtOH were not significantly different from each other in the PbN. The number of cFLI in response to stimulation solution was not different from that of DDW in the NAcc. The result of the present study suggests that not only sucrose but also EtOH activates some neurons in the PbN, and that some pontine neurons possibly respond to both sucrose and EtOH.

The aim of this study was to investigate the antioxidant activity of thermal treatment yam (Dioscorea batatas DECNE.) in Korea. Thermal treatment yam was extracted by different solvents including 70% methanol, 70% ethanol and chloroform–methanol mixture (CM, 2:1, v/v). Then color property, total phenol content and antioxidant activity were analyzed. Yam possessed high L* value and H° value, which were 54.92±2.18 and 73.20±0.77, respectively. Thermal treatment yam exhibited great antioxidant activity evaluated by ABTS [2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, and ferric reducing antioxidant power. Total phenol contents of various extracts from thermal treatment yam increased in the following order: 70% methanol extract (63.53±0.33 mg CAE/g), 70% ethanol extract (69.47±1.00 mg CAE/g) and CM extract (97.49±0.66 mg CAE/g), respectively. The same trend was also could be found in antioxidant activity assays except for reducing power assay. These results implied that these extracts from thermal treatment yam might be useful to take a good part in prevention of human diseases and aging.

In this study, the thermal treatment yam (Dioscorea batatas DECNE.) was analyzed for its proximate and nutritional compositions including mineral, vitamin, amino acids and fatty acids. Thermal treatment yam is mainly consisted of carbohydrate (70.3%) with small amounts of crude protein (15.8%), crude ash (5.1%) and crude fat (1.6%). It was found to be a good source of essential minerals such as K (1100.2 mg/100 g), Mg (99.4 mg/100 g), Ca (54.8 mg/100 g) and Na (44.9 mg/100 g) but Zn (4.8 mg/100 g) and Fe (0.2 mg/100 g) content was low. Little amounts of vitamin E (8.3 mg/100 g), vitamin C (3.5 mg/100 g) and vitamin B1 (2.1 mg/100 g) could be found. The amino acid analysis revealed that the yam was superior with respect to serine (1454.2 mg%), lysine (684.6 mg%) and histidine (684.6 mg%). Essential amino acids were calculated to be 2849.3 mg%. The amino acid profiles showed that thermal treatment yam is limiting in isoleucine and phenylaline. Linoleic acid was the most predominant fatty acids with the value of 47.3% followed by palmitic acid (24.7%) and oleic acid (12.7%). And the unsaturated fatty acids including oleic acid and linoleic acid were present in big quantities in thermal treatment yam.

We map two molecular clouds located in the exact anticenter region emitting in the (J = 1-0) transition of 12CO and 13CO using the 3-mm SIS mixer receiver on the 14-m radio telescope at Taeduk Radio Astronomy Observatory. The target clouds with anomalous velocities of VLSR ~ - 20 km s-1 are distinguished from other clouds in this direction. In addition, they are located in the interarm region between the Orion Arm and the Perseus Arm. Sizes of the clouds are estimated to be about 8.6 and 10.8 pc, respectively. The total mass is estimated to be about 4 x 103 M⊙ using CO luminosity of the clouds. Several cores are detected, but no sign of star formation is found according to the IRAS point sources. Their larger linewidths, anomalous velocities, and their location at the interarm region make these clouds more distinguished, though their physical properties are similar to the dark clouds in the solar neighborhood in terms of mass and size.

Lactic acid bacteria were cultivated from the gut of insects and analyzed. The gut samples were obtained from Anoplocnemis dallasi, Apis mellifera, Diestrammena coreana, Gonolabis marginalis, and Mycalesis gotama. 16S rRNA gene sequence analysis revealed that the culture-dependent lactic acid bacteria isolated from the insect gut samples belonged to the genera Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, and Weissella. The genera Enterococcus and Lactococcus were dominant, constituting 57% and 25% of the isolated strains, respectively. The distribution of lactic acid bacteria was found to differ according to the insect species. However, because culture-dependent methods identify only a portion of lactic acid bacterial communities, the use of culture-independent methods in future studies will be required to complement the methods used in the present study.

The aim of this study was to develop PCR primer sets for the rapid classification of lactic acid bacteria (LAB). Published gene sequences for LAB carbohydrate metabolic enzymes were collected from the NCBI GenBank, and 45 primer sets were designed using the gene sequences. Twelve species of LAB were used as reference strains: Lactobacillus brevis, Lactobacillus farciminis, Lactobacillus fermentum, Lactobacillus hilgardii, Lactobacillus plantarum, Weissella cibaria, Weissella confusa, Weissella kimchii, Leuconostoc citreum, Leuconostoc lactis, Pediococcus acidilactici, and Pediococcus pentosaceus. PCR amplification and gel electrophoresis were performed to verify the specificities of the designed primers. The results showed that the primer set No. 1 of 5'-aacaacaaaatcaccgcaca-3' & 5'- gtcgtcaatgttgtcgatgc-3' for phosphofructokinase amplified PCR products from 5 species of heterofermenter with different molecular weight depending on the genus. Primer set No. 8 of 5'-gcgtcgccgtctcg-3' & 5'- gcctgcggcttttcg-3' produced specific PCR products from three heterofermentative lactobacilli such as L. brevis, L. fermentum, and L. hilgardii. Primer set No. 18 of 5'-gtgacggtgctgtaggttca- 3' & 5'-gcagtcgcttacgccatatt-3' was specifically reacted to homofermentative species including L. farciminis, P. acidilactici, and P. pentosaceus. Hence, the primer sets which were developed in this study could be used as a tool for the classification and differentiation of homo- and hetero fermentative species in lactic acid fermented foods, like as kimchi.

A progressive failure analysis procedure for composite laminates is developed in here and in the companion paper. An anisotropic plastic constitutive model for fiber-reinforced composite material, is developed, which is simple and efficient to be implemented into computer program for a predictive analysis procedure of composites. In current development of the constitutive model, an incremental elastic-plastic constitutive model is adopted to represent progressively the nonlinear material behavior of composite materials until a material failure is predicted. An anisotropic initial yield criterion is established that includes the effects of different yield strengths in each material direction, and between tension and compression. Anisotropic work-hardening model and subsequent yield surface are developed to describe material behavior beyond the initial yield under the general loading condition. The current model is implemented into a computer code, which is Predictive Analysis for Composite Structures (PACS), and is presented in the companion paper. The accuracy and efficiency of the anisotropic plastic constitutive model are verified by solving a number of various fiber-reinforced composite laminates with and without geometric discontinuity. The comparisons of the numerical results to the experimental and other numerical results available in the literature indicate the validity and efficiency of the developed model.

Scarabaeus typhon has the extensively wide distribution from Europe to the Far East Asia and the extremely similar morphological feature with closely related species. The Korean population of S. typhon had often been confused in its specific status including several misidentifications and synonymous name. By these circumstances, we presumed that there is a possibility to exist potential cryptic species or subspecies, which might be separated between local populations across their distribution range. Therefore, the primary aim of this study was to reassess that the geographical populations of S. typhon can be divided into each other and to establish a sketchy knowledge of its unknown phylogenetic relationships between the relatives using COI gene and comparative morphology. As the result, S. typhon was detected as a single species despite to have the wide distribution and the various intraspecific distances ranging from 0.67% to 3.50%. Two species, S. pius and S. babori were revealed to have two distinct lineages respectively. Among them, two Korean female specimens were detected belonging to group B of S. pius, it is suggested as a cryptic species or subspecies.

Insects impact human health through vector-borne diseases and cause major economic loses through damaging crops and stored agricultural products. Insect-specific growth regulators (IGR) represent attractive control agents because of their safety to the environment and humans. Here, we report identification of plant compounds that are antagonists of the insect-specific juvenile hormone (PJHANs), using the yeast two hybrid system transformed with the mosquito JH receptor as a reporter assay. We show that these compounds act by inhibiting larval growth and reproduction in mosquitoes. We also demonstrate that PJHANs affect the JH receptor, Methoprene-tolerant (Met), by disrupting its complex with CYCLE, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants, Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Two of these diterprenes affect Met function, leading to reduction in expression of Met target genes and causing retardation of follicle development in mosquito ovaries. Developing potent compounds counteracting JHaction (JH antagonists) would find a wider range of control applications. However, so far such JH antagonists have not been developed. Here, we report the discovery of potent JH antagonists in plants, which represents an innate resistance mechanism of plants against insect herbivores. These newly discovered plant JH antagonist compounds could be used as the starting material for developing novel insecticides.

In this study, we optimized dissolution the dissolution conditions of porous amorphous powder to havehigh specific surface area. Porous metallic glass(MG) granules were fabricated by selective phase dissolution, in whichbrass is removed from a composite powder consisting of MG and 40 vol.% brass. Dissolution was achieved throughvarious concentrations of H2SO4 and HNO3, with HNO3 proving to have the faster reaction kinetics. Porous powderswere analyzed by differential scanning calorimetry to observe crystallization behavior. The Microstructure of milledpowder and dissolved powder was analyzed by scanning electron microscope. To check for residual in the dissolvedpowder after dissolution, energy dispersive X-ray spectroscory and elemental mapping was conducted. It was confirmedthat the MG/brass composite powder dissolved in 10% HNO3 produced a porous MG granule with a relatively high spe-cific surface area of 19.60 m2/g. This proved to be the optimum dissolution condition in which both a porous internalgranule structure and amorphous phase were maintained. Consequently, porous MG granules were effectively fabricatedand applications of such structures can be expanded.

This research tends to answer the following question: How do multinational corporations transmit their service brand to the local employees of the host country? Service brand has been discussed from the point of internal marketing, which ensures that employees can demonstrate the authentic value of the service brand in their attitudes and behaviors. However, when a corporation attempts to transmit the service brand across borders, there needs to be a balance between globalization and localization. This research intends to build a systematic internal marketing mechanism from the viewpoint of internal marketing, international service branding and subsidiary brand-specific transformational leadership. This study uses both qualitative and quantitative methods to conduct an in-depth case study and questionnaire survey. This research provides the following theoretical and managerial contributions. Firstly, it proposes a systematic internal marketing mechanism to transmit the service brand across international borders. This includes a brand-specific leadership with cultural sensitivity, a brand norm positively led by the leader and an organizational learning system, which influences the practice of the brand norms. Secondly, in order to transmit the international service brand successfully, this paper argues that subsidiaries’ leaders should have cultural sensitivity and demonstrate a brand-specific transformational leadership style. Thirdly, the paper finds and verifies that brand-specific transformational leadership has a positive effect on the brand norms and organizational learning, which in turn affect employee brand behavior and attitude toward the company’s core brand value. Managerially, the systematic internal marketing mechanism developed by this research can enlighten those service companies who intend to expand their international scope and ascertain their service brand value is transmitted accurately and executed locally.

This study explores the relationship among brand experience, consumer satisfaction and brand loyalty with virtual experience and the virtual community as the intervening variables. We intend to discuss how the new concept of ‘brand experience’ impacted by the internet environment. We integrate the following new research subjects together: brand experience, virtual experience and virtual community. As the research purpose is to understand the effects of virtual environments on brand experience on consumer satisfaction and on brand loyalty, it takes virtual experience as the intervening variable to discuss whether it positively or negatively influences the relationship between brand experience and consumer satisfaction, and takes the virtual commodity variable as the intervening variable to discuss whether it positively or negatively influences the relationship between consumer satisfaction and brand loyalty. This study takes the virtual community members of apple brand in taiwan as the research subjects and 516 questionnaires were completed and returned. The results show that the brand experience had a positive effect on the consumers’ satisfaction and brand loyalty. In addition, the virtual experience has a positive moderating effect on the relationship of brand experience and consumer satisfaction. Finally, this study also discovered that the virtual community also has a positive moderating effect on the relationship between consumers’ satisfaction and brand loyalty.

We apply differential affine velocity estimator (DAVE) to the Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager (HMI) 12-min line-of-sight magnetograms, and separately cal- culate the injected magnetic helicity for the leading and the following polarities of nine emerging bipolar active regions (ARs). Comparing magnetic helicity ux of the leading polarity with the following polarity, we nd that six ARs studied in this paper have the following polarity that injected more magnetic helicity ux than that of the leading polarity. We also measure the mean area of each polarity in all the nine ARs, and nd that the compact polarity tend to possess more magnetic helicity ux than the fragmented one. Our results conrm the previous studies on asymmetry of magnetic helicity that emerging bipolar ARs have a polarity preference in injecting magnetic helicity. Based on the changes of unsigned magnetic ux, we divide the emergence process into two evolutionary stages: (1) an increasing stage before the peak ux and (2) a constant or decreasing stage after the peak ux. Obvious changes on magnetic helicity ux can be seen during transition from one stage to another. Seven ARs have one or both polarity that changed the sign of magnetic helicity ux. Additionally, the prevailing polarity of the two ARs, which injects more magnetic helicity, changes form the following polarity to the leading one.

The purpose of this study is to evaluate salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva of the head and neck cancer patients. Twenty three cancer patients (19 males, 4 females) who had undergone chemotherapy and radiation therapy and twenty four healthy volunteers (14 males, 10 females) as a control were included. Salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva were examined. Compared to saliva of the control group, salivary flow rate (p<0.001) and salivary pH (p<0.001) were significantly lower in head and neck cancer patients. The colony counts of Lactobacilli was higher in head and neck cancer patients (p<0.05) than in control group. These salivary factors and cariogenic activity can increase the prevalence of dental caries in head and neck cancer patients.