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        검색결과 75

        41.
        2012.12 KCI 등재 서비스 종료(열람 제한)
        Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.
        42.
        2012.07 서비스 종료(열람 제한)
        Korean soybean variety Kwangan was transformed with coat protein (CP), helper component-proteinase (HC-Pro), and ABRE binding factor 3 (ABF3) genes using highly efficient soybean transformation system. Among these genes, CP and HC-Pro were transformed using RNAi technology. Transgenic plants with CP were confirmed for gene introduction and their expression using PCR, real-time PCR, RT-PCR, Southern blot, and Northern blot. To investigate the response of viral infection with CP, T1 plants were inoculated with SMV-infected leaves and confirmed the existence of mosaic symptom in both leaves and seeds. Two transgenic lines with CP were highly resistant to SMV with clear leaves and seeds while SMV-susceptible lines showed mosaic symptom with seed mottling. The transcript levels of T1 plants with CP were also determined by northern blot, suggesting that SMV-resistant T1 plants did not show viral RNA expression whereas SMV-susceptible T1 plants showed viral RNA expression. Currently, the response of viral infection with HC-Pro is investigating to produce SMV-resistant soybean transgenic plants, and the physiological experiment with ABF3 is also carrying out to produce drought-tolerant soybean transgenic plants.
        52.
        2007.12 KCI 등재 서비스 종료(열람 제한)
        A new cultivar of Phalaenopsis, "Snow Dream" was initially selected from the progenies of the cross of Phal.3016xPhal.3021 (no name, collection at the nursery) in 1996 at the National Horticultural Research Institute, Rural Development Administration. Final selection was made in 2003 after the investigation of the characteristics for five years (1999-2003). "Snow Dream" has long blooming (about 4months), white flower, pink stripe lip, semi-upright leaf. PLB(Protocorm Like Body) induction rate was 70%. The width of flower is 9.0 cm. The direct sun light should be avoided for its appropriate growth. Growing temperatures should not be exceed 35℃/15℃ (day/night). A variety protection for "Snow Dream" has been applied in 2005.
        53.
        2007.12 KCI 등재 서비스 종료(열람 제한)
        A new cultivar of Phalaenopsis, "Orange Dream" was initially selected from the progenies derived from self-pollination of 3091(no name) in 1996 at the National Horticultural Research Institute, Rural Development Administration. It was finally selected in 2003 after the investigation of the characteristics for five years (1999-2003). "Orange Dream" has orange color and purple line with thick petal, sepal, and long blooming, semi-upright leaf and high-mass propagation of 70%. The width of flower is 6.5 cm with medium sized and the number of flowers is 6. Strong sunlight should be avoided for its growth. Growing temperatures of 35℃/15℃(day/night) are advisable. A variety protection has been applied for this cultivar in 2005.
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