담배포장에서 담배나방의 발생은 조식 및 만식 재배구 모두에서 2 또는 3개의 뚜렷한 최성기를 보였다. 반면에 Campoleitis chlorideae는 담배 조식구에서는 담배나방의 두번째 최성기 직후 짧은 기간동안 발생하였으며 만식구에서는 담배나방 유웅이 존재하는 한 발생하였다. 만식구에서의 담배나방 발생은 조식구에서보다 많았다. 4가지 담배 품종중 NC-744에서 담배나방 유충의 발생이 비교적 컸다. 그러나, 기생벌의 발생은 Burley-21과 NC-82에서 많았다. 담배나방 유충의 포장에서의 인위적인 방사 및 재포획법에 의한 결과는 C. chlorideae의 활동은 6월말부터 9월초까지 보였으며 7월초 부터 8월말까지는 비교적 높은 밀도로 발생하였다. C. chlorideae의 담배나방에 대한 생물적방제 수단으로서의 가치평가를 위한 포장내 소규모 케이지 실험결과 방사밀도가 높을수록(암컷 4마리/2 ) 유충기생률이 높고(86.1%) 담배잎의 피해는 감소함을 보였다.(8.7%) 무처리구에서의 담배잎의 피해는 상당히 높았으며(23.2%) 살충제처리구에서 피해가 가장 낮았다.(1.6%)

Theoretical profiles of selected rotational lines of C 2 CH, CN, TiO and MgH are computed by using the current models of sunspot unbrae and penumbrae. It is found that the lines of the diatomic carbides are enhanced in penumbrae relative to umbrae, while MgH lines are more strongly enhanced in umbrae than in penumbrae and the quiet photosphere. The results are discussed with respect to selecting lines suitable for studying the structure of sunspots.

Surface density distributions for globular clusters were obtained from photoelectric surface photometry (using centered aperture photometry). These surface brightness profiles were then compared with the theoretical surface density distribution of King's model. From the comparison of the theoretical and observed surface density distributions, we determine he structural parameters of the clusters (the core radius r c , the tidal cut off r t , and the concentration factor C).

Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.

수도 생육기간중 이상저온이 내습하였던 1980년에 동일위도상의 표고가 다른 두 개 지역, 수원(127˚ , 37˚ 20' 표고 37m)과 제천 (128.2˚ , N37˚ 10', 표고 280-300m)에다 Japonica 품종인 대골도, 진흥, 신002와 통일형품종 조생통일, 수원 287호(태백벼), wx 817-1-65-2-3(서울대 농대육성) 등을 4월 21일 파종 6월 1일 이앙하고 출수기, 간장, 수장 및 수량 관련형질들을 조사 비교 검토하였다. 1. 간장, 수장 및 1,000립중은 Japonica품종의 경우는 제천에서 통일형 품종의 경우는 수원에서 약간 작아지는 경향이었으나 유의적인 차이는 아니었다. 2. 수량구성요소중 주당 수수는 제천에서 현저히 증가되었는데 그 정도는 품종에 따라서 달랐으며, 임실율은 수원이 제천보다, Japonica 품종이 통일형 품종보다 높았는데 지역간 차이는 통일형 품종에서 현저하였다. 3. 지역에 따른 품종들의 품종군에 따라 달라 Japonica 품종들(대골도 제외)은 제천에서 월등히 높았으나 통일형 품종들은 제천에서 모두 감수되었다.