Recent corpus-based approaches have emphasised autonomous and process-oriented methodologies focused on developing learners’ cognitive skills. This cognitive development is based on consciousness-raising being considered a necessary condition for language learning (Schmidt, 1990). From this viewpoint, the study was aimed at how this kind of corpus-based approach contributes to EFL learners’ interlanguage development. To this end, the study examined advanced Korean learners’ acquisition of English determiners via a corpus-based methodology designed to enhance their cognitive ability and skills. To achieve this, the study contrastively analysed two methodologies: traditional structuralist and corpus-based. The former focused on a product-oriented perspective which is commonly used in Korean grammar classes, while the latter focused on a process-oriented perspective which was based on learners’ exploration of corpus data. The study discovers that both methodologies lead learners to raise their consciousness of knowledge about the grammatical rules of language, but the corpus-based methodology also raises learners’ consciousness of the knowledge of how language is used at the level of noticing. This result suggests that this level of consciousness, raised via a process-oriented corpus methodology, can contribute to EFL learners’ interlanguage development.

Pregnancy is a unique event in which a fetus develops in the uterus despite being genetically and immunologically different from the mother, and the underlying mechanisms remain poorly understood. To analyze the differential gene expression profiles in nonpregnant and 7 days post coitus (dpc) pregnant uterus of mice, we performed a global proteomic study by 2‐D gel electrophoresis (2‐DE) and MALDI‐TOF‐MS. The uterine proteins were separated using 2‐DE. Approximately 1,000 spots were detected on staining with Coomassie brilliant blue. An image analysis using Melanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between pregnant and nonpregnant uterus. Twenty‐one spots were identified as differentially expressed proteins, of which 10 were up‐regulated proteins such as alpha‐fetoprotein, chloride intracellular channel 1, transgelin, heat‐shock protein beta‐1, and carbonic anhydrase II, while 11 were down‐regulated proteins such as X‐box binding protein, glutathione S‐transferase omega 1, olfactory receptor Olfr204, and metalloproteinase‐disintegrin domain containing protein TECADAM. Most of the identified proteins appeared to be related with catabolism, cell growth, metabolism, regulation, cell protection, protein repair, or protection. Our results uncovered key proteins of mouse uterus involved in pregnancy.

14-3-3 proteins are known to play a pivotal role in a diverse array of cellular events such as cell survival, apoptosis, and signal transduction. Numerous 14-3-3 ζ have been cloned and characterized from a host of eukaryotic organisms including human, plants, yeast, fruit fly and silkworm. However, no study on Spodoptera exigua 14-3-3ζ in conjunction with virus infection has so far been reported in insects. It appears that expression of Se14-3-3ζ was decreased starting 24 h post-SeNPV infection as SeNPV titers seemed to increase as evidenced by intense bands of SeNPV IAP3. Interestingly, confocal microscopic analysis revealed that Se14-3-3ζ is expressed at the apical side of the NPV-uninfected gut cells, whereas it was detected mainly in the nucleus of the NPV-infected cells. Thus, despite the biological significance of Se14-3-3ζ in S. exigua in conjunction with molecular interactions between SeNPV and S. exigua is unclear now, our data suggest that Se14-3-3 ζ protein plays a role to protect S. exigua from the infection or inhibit replication of SeNPV.

Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway is known to play a pivotal role in various cellular events and antiviral responses in both vertebrates and insects. In an attempt to elucidate the potential involvement of STAT on S. exigua-SeNPV interactions, the full length cDNA of SeSTAT was cloned from S. exigua. Analysis of temporal expression patterns shows that SeSTAT is expressed in all stages of life cycle such as larvae, pupae, and adult. Spatial expression analysis shows that it is highly expressed in fat body and Malpighian tubule. Interestingly, SeSTAT is induced at 24 h in response to either laminarin or LTA injection in larvae. Electrophoretic mobility shift assay (EMSA) shows that the binding of nuclear extracts from fat body cells immune-challenged with LTA to STAT5 probe was observed. In addition, SeSTAT was nuclear-translocalized in both fat body and gut cells that were challenged with LTA and laminarin, respectively. Finally, gene silencing of SeSTAT shows that SeNPV number appears to be increased. It suggests that SeSTAT may act as a negative regulator against SeNPV in midgut.

Matsumuraeses phaseoli and M. falcana cannot be classified with the external morphological characters. Although differences in the morphology of male genitalia and in the mitochondrial DNA sequence of cytochrome oxidase I between the two species have been found, there is no information that the two species are biologically different species till now. We, therefore in this study, tried to cross the two species to observe postzygotic incompatibility in the next hybrid generations and to know whether the two species are 'biological species' or not. In crossing between the parents, two kinds of F1 hybrids were produced successfully. In inter- and intra-breeding between F1 hybrids, two lines crossed with females of F1 hybrid produced by females of M. phaseoli could not produce F2 adults to show inviability of larvae. The other two lines produced F2 adults successfully and the F2 adults produced F3 larvae in inbreeding. In back-crossing between parents and F1 hybrids, the two lines of 8 breeding lines, in which females of F1 hybrid were produced by females of M. phaseoli, could not produce the next generation of larvae. The other six lines produced F2 adults successfully. The results indicated that maternal factors of F1 hybrid produced by M. phaseoli females contributed to create the incompatibility between the F1 females and other lines. In conclusion, the results showed a postzygotic reproductive isolation between M. phaseoli and M. falcana in part.

Many biological systems are regulated by an intricate set of feedback loops that oscillate with a circadian rhythm of roughly 24 h. This circadian clock mediates an increase in body temperature, heart rate, blood pressure, and cortisol secretion early in the day. Recent studies have shown changes in the amplitude of the circadian clock in the hearts and livers of streptozotocin (STZ)-treated rats. It is therefore important to examine the relationships between circadian clock genes and growth factors and their effects on diabetic phenomena in animal models as well as in human patients. In this study, we sought to determine whether diurnal variation in organ development and the regulation of metabolism, including growth and development during the juvenile period in rats, exists as a mechanism for anticipating and responding to the environment. Also, we examined the relationship between changes in growth factor expression in the liver and clock-controlled protein synthesis and turnover, which are important in cellular growth. Specifically, we assessed the expression patterns of several clock genes, including Per1, Per2, Clock, Bmal1, Cry1 and Cry2 and growth factors such as insulin-like growth factor (IGF)-1 and -2 and transforming growth factor (TGF)-β1 in rats with STZ-induced diabetes. Growth factor and clock gene expression in the liver at 1 week post-induction was clearly increased compared to the level in control rats. In contrast, the expression patterns of the genes were similar to those observed after 5 weeks in the STZ-treated rats. The increase in gene expression is likely a compensatory change in response to the obstruction of insulin function during the initial phase of induction. However, as the period of induction was extended, the expression of the compensatory genes decreased to the control level. This is likely the result of decreased insulin secretion due to the destruction of beta cells in the pancreas by STZ.

18th of May means the memorial (symbolic) day for Kwang-Ju citizens who resisted against Korean army in order to democratize South Korea in 1980. <Fig.1> illustrates the still image of 5.18 Kwang-Ju affairs. This work was shown in Hub City of Asian Culture Project, “Integrating”, administered by the national government of Korea in Kwang-Ju, 2007, for memorial of 5.18 Kwang-Juaffairs[1]. As the proverb says, “It's better to see it once than hearing it a thousand times.” Eyes as the visual organ play an important role in order to operate human sense organ. These visual images of 5.18 affairs are aroused from their resistance souls and reported for the live scene in those days. Indirectly we are able to feel an emotion of tremble like a fear of 5.18. Inspiration of visual scene propagates through human brain-waves.

The Maillard Reaction Products (MRPs) such as Glucose-tyrosine (Glu-Tyr) and Xylose-arginine (Xyl-Arg) have antioxidant, antimutagenic, and antibacterial effects. However, to date, still little is known about the other biological effects of the MRPs. In this study, we investigated whether the fructose-tyrosine MRP, 2,4-bis(p-hydroxyphenyl)-2-butenal (Fru-Tyr), could modulate cell cycle progression and NF-κB activity, and thereby induce apoptotic cell death of colon cancer cells. Treatment with different concentrations (10-40 μg/ ml) of Fru-Tyr for 24 h inhibited colon cancer cell (SW620 and HCT116) growth followed by induction of G₂/M phase cell cycle arrest and apoptosis in a dose-dependent manner. We also found that Fru-Tyr suppressed tumor necrosis factor-alpha (TNF-α)-induced NF-κB transcriptional activity. Moreover, Fru-Tyr induced the expression of apoptotic gene, cleaved caspse-3. These results suggest that Fru-Tyr inhibited colon cancer cell growth through induction of G₂/M phase cell cycle arrest and apoptotic cell death by modulating of NF-κB.

To evaluate the hypolipidemic effects of Cheonggukjang (CGJ), which is frequently used in Korea similar to Natto in Japan and Douchi in China like a dairy product, boiled soybeans were fermented with two Bacillus strains, B. subtilis and B licheniforms, is이ated from rice straw and their antihyperlipidemic effects oftheir products were investigated. Treatment with the CGJs significantly reduced blood triglyceride (TG) and total ch이esterol (TC) levels and increased HDL cholesterollevels in Triton WR-l339-induced hyperlipidemic mice. The treatment of non-fermented soybeans alone also reduced blood TG and TC levels, but not significantly. Feeding the CGJs significantly lowered high blood TG and TC levels as well as body and epididymal mass weights in hyperlipidemic mice induced by the long-term feeding of a high-fat diet that increased blood HDL cholesterollevels. The B. subtilis-fermented CGJ products more potently reduced TG and TC levels, although the differences between the starters were not significant. These finding suggest that CGJ products may be effective as hypolipidemic foods by the synergistic interaction of soy and Bacillus strains.

This study was conducted to evaluate the attractiveness of the sex pheromone trap of Cnaphalocrocis medinalis Guenée as a forecasting tool from 2006 to 2008 in Suwon, Gyeong-gi province and Seosan, Chung-nam province, Korea. The sex pheromone lure was composed of Z11-18:Ald (55㎍): Z13-18:Ald (500㎍): Z11-18:OH (120㎍): Z13-18:OH (180㎍): Z13-18:Ac (55㎍) per a rubber septum, which set in a cylindrical cone trap. The trap catches of male moths was counted on a weekly basis and the lures were renewed every two weeks. The flight activity of C. medinalis moths monitored by the sex pheromone trap revealed two distinct peaks a year in both experimental areas from 2006 to 2008. Degree-days accumulation was calculated using lower developmental threshold (13.3℃) and degree-days (386.4DD) since adults had been first recorded. Estimated adult peak of the first generation was almost coincident with that observed by sex pheromone trap except in Suwon in 2008.

Apolipophorin-Ⅲ (apoLp-Ⅲ) is a hemolymph protein whose function is to facilitate lipid transport in an aqueous medium in insect. Recently, apolipophorin-Ⅲ in Galleria mellonella and Hyphantria cunea was shown to play an unexpected role in insect immune activation. We show here a novel possible function/role of apoLp-Ⅲ in insects. To investigate the genes which have a relationship with apoLp-Ⅲ in fall webworm larvae, we reduction of endogenous Hc apoLp-Ⅲ mRNA levels in larvae via RNA interference (RNAi). The RNAi-mediated Hc apoLp-Ⅲ reduction resulted in the reduction of antioxidants, like MnSOD, catalase, and glutathione S transferase as well as immune proteins. In particular, expression of MnSOD commonly decreased in fat body, midgut, and hemocytes following the knockdown of Hc apoLp-Ⅲ, which induced an elevated level of superoxide anion in H. cunea larvae. The observed effect of Hc apoLp-Ⅲ RNAi suggests that Hc apoLp-Ⅲ is related to the action/expression of antioxidants.

Innate immunity responses are triggered by the immune challenge and therefore involve signaling processes. The cellular response is initiated by hemocytes and mainly involves phagocytosis and encapsulation of intruders by these cells. To address whether Hc-STAT is activated upon bacterial challenge, we examined the subcellular location of STAT protein in hemocyte by immunostaining. A new insect member of the STAT family of transcription factors (Hc-STAT) has been cloned from the lepidopteran, Hyphantria cunea. The domain involved in DNA interaction and the SH2 domain are well conserved. The gene is transcribed at a low level during all stages of development, and the protein is present in hemocytes, fat body, midgut, epidermis, and Malphigian tuble (Mt). Especially, hemocytes and Mt showed transcriptional activation of Hc-STAT upon Gram (-) bacteria and fungal challenge. Gram (-) bacteria and fungal challenge specifically results in nuclear translocation of Hc-STAT protein and induction of DNA-binding activity that recognizes a STAT target site in H. cunea hemocyte. In vitro treatment with pervanadate translocates Hc-STAT to the nucleus in hemocyte cells. Here we report the first evidence for the involvement hemocyte JAK/STAT pathway upon microbial infection in lepidopteran insect.

Up to now, 39 species of the family Anthribidae have been recorded from Korea. As a result of this study, we add two genus and four species of the family Anthribidae to the Korean fauna with brief descriptions and illustrations of diagnostic characters. The newly recorded genus Citacalus in the subfamily Choraginae, is very similar to the genus Choragus. Citacalus pygidialis Johraku has an unique character, pygidium strongly convex longitudinally sharply carinate on the apical half in male; in female, pygidium triangularly projecting laterally near the apex. The newly recorded species Euparius modicus yaeyamanus Morimoto in the genus Euparius of the subfamily Anthribinae, can be distinguished from the other two recorded species by the elytra with almost unicolor pubescence color except a pair of black spots behind middle. The newly recorded genus Unciferina is close to Uncifer Jordan, but easily distinguished from the latter by sharply angulate rostrum to head, transversely carinate base of rostrum on the underside, and the acute hind angles of pronotum. The two new species in the genus Unciferina are easily distinguished from the congeneric species with pubescence patches on pronotum and elytra, shape of eye, the ratio of the antennal segments and the shape of the hind angles of pronotum.

(E,E)-8,10-dodecadienyl acetate (E8E10-12:Ac) and (E)-8-dodecaenyl acetate (E8-12:Ac) have been selected as the candidate chemicals for sex pheromone components of the M. phaseoli, female through GC-EAD tests, whereas the two compounds and an additional candidate, (E,Z)-7,9-dodecadienyl acetate (E7Z9-12:Ac), have been found at a ratio of 7:1:1 in the abdominal tip extract (Yum et al., 2008). In order to determine the actual composition of sex pheromone, therefore, several blends using the three chemicals were evaluated for attractiveness to males of M. phaseoli around red bean and soybean fields. Individual components as well as two blends consisted of E8E10-12:Ac/E7Z9-12:Ac and E8-12:Ac/E7Z9-12:Ac did not show attractiveness, whereas the blend of E8E10-12:Ac/E8-12:Ac showed an increased effect in male capture. Of the tested blends with all three chemicals, the 7:1:2 composition of E8E10-12:Ac, E8-12:Ac and E7Z9-12:Ac attracted the most number of males. The results suggested that E7Z9-12:Ac is one of the sex pheromone components and may act as a synergist.

The root zone applications of a systemic insecticide, carbofuran, were evaluated for their impacts on the brown planthopper, Nilaparvata lugens (Stål), and spider populations in the greenhouse and rice paddy fields. In the green house experiments, no BPH nymphs were hatched at root zone treated on 40 to 50 day-old rice, while around 20 to 54 nymphs per pot were emerged in broadcasting and foliar spray treatments. This indicates that the root zone treatment can kill the eggs of BPH effectively. This is the first study ever demonstrated the high egg mortality of BPH due to the root-zone application. In the field experiments, the density of BPH in root zone treated plots were four to six times lower than in broadcasting and foliar spray plots at the 21 days after application. The BPH outbreaks and hopper-burns were observed at all treatments except the root zone treated plot at the 28 days after application. The root-zone application did not impact on the spider population, while foliar spray killed most of all spiders just one day after application. The densities of spider in foliar spray plots were always lower than in root-zone treated and control plots. The results indicated that the root-zone application of carbofuran can control BPH effectively without adverse effects to the spiders inhabited on the paddy field.