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        검색결과 450

        64.
        2021.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, we investigated the difference in the affective characteristics between science-gifted students and general students through the positive experiences about science (PES) index. We also explored ways to apply the characteristics of gifted classes suggeseted by the teachers of this study, which had a positive effect on science-gifted students, to general science classes. For this study, a PES survey was carried on middle school science-gifted students enrolled in the gifted education center in the central region and general middle school students in the same area who had no experience in gifted education. Based on the survey result, we conducted in-depth interviews with teachers, having teaching experience with both science-gifted and general students. The results revealed that science-gifted students showed a significantly higher PES index than general students in all five areas of PES. The area with the largest difference between the two groups was science-related self-concept and the smallest was science academic emotion. Teachers suggested ways to apply the characteristics of science-gifted classes to general science classes, such as organizing general science classes around inquiry activities, supporting class materials such as MBL or tablets, reconstructing the classes using materials reflecting students’ needs, and changing the textbook content and narrative style, to induce students' interest and curiosity. Based on the study results, ways to enhance the PES through science classes for general students were proposed.
        4,200원
        65.
        2021.06 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        Even in an era where 8-meter class telescopes are common, small telescopes are considered very valuable research facilities since they are available for rapid follow-up or long term monitoring observations. To maximize the usefulness of small telescopes in Korea, we established the SomangNet, a network of 0.4{1.0 m class optical telescopes operated by Korean institutions, in 2020. Here, we give an overview of the project, describing the current participating telescopes, its scienti c scope and operation mode, and the prospects for future activities. SomangNet currently includes 10 telescopes that are located in Australia, USA, and Chile as well as in Korea. The operation of many of these telescopes currently relies on operators, and we plan to upgrade them for remote or robotic operation. The latest SomangNet science projects include monitoring and follow-up observational studies of galaxies, supernovae, active galactic nuclei, symbiotic stars, solar system objects, neutrino/gravitational-wave sources, and exoplanets.
        4,600원
        67.
        2021.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Resistance to extended-spectrum cephalosporin in Enterobacteriaceae is increasingly prevalent in South Korea. This study aims to explore the distribution of AmpC genes in Proteus mirabilis isolated from stray and hospital-admitted companion animals in South Korea. AmpC β-lactamases hold clinical significance due to its potential to facilitate antimicrobial resistance to cefoxitin, cefazolin, and most penicillins. A total of 163 bacterial isolates belonging to the Enterobacteriaceae family were collected from dogs (n = 158) and cats (n=5). Of them, 134 isolates were from hospital-admitted animals, while 29 isolates from stray animals. Boronic acid tests and antimicrobial susceptibility tests were conducted for an initial screening to detect AmpC β-lactamase resistant P. mirabilis. Gene-specific PCRs were conducted to identify the type of AmpC genes, which include six groups (MOXM, CITM, DHAM, ACCM, EBCM, and FOXM), in the resistant isolates. The boronic acid disk tests revealed 45 (27.6%) positive isolates out of 163 isolates tested. Of these 45 isolates, six were determined to harbor the EBCM gene, 13 for CITM, one for FOXM, and one for DHAM by single detection PCR. No isolates carried for ACCM or MOXM. Thus, a total of 21 out of 163 isolates (12.9%) were demonstrated to possess AmpC genes. No isolates contain more than one group of AmpC gene family. A significantly higher percentage of P. mirabilis was found to possess AmpC genes compared to past studies. Therefore, the increasing trend in antimicrobial resistance in P. mirabilis indicates a dire need to monitor antimicrobial prescription in the veterinary field.
        4,000원
        68.
        2020.12 KCI 등재 구독 인증기관·개인회원 무료
        시설토마토에서 사전에 해충밀도를 예찰 하지 않고 작물 정식과 동시에 적용한, 기생성/포식성 천적과 그들의 먹이원/서식처가 결합되어 있는, ‘Natural Enemy in First (NEF)’ 기술의 총채벌레와 진딧물 방제효과를 확인하였다. 처리 후 12주차에 NEF 처리구에서 총채벌레 밀도억제 효과는 천적처리구 및 관행방제구에 비해 각각 32%와 82% 더 높았다. 처리 후 진딧물의 밀도는 모든 처리구에서 낮게 유지되어 처리구간 유의성 있는 차이를 확인할 수 없었다.
        69.
        2020.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The present study investigated the intra-vitam larval migration of Anisakis pegreffii, which is naturally infected, from the intestine to the viscera and the musculature of live spotty-bellied greenling, Hexagrammos otakii, under the different seawater temperatures. The results showed that the increase in the ratio of larval migration from the intestine to the viscera correlated with the increase in the water temperature in the aquarium. The intra-vitam larval migration ratios from the intestine to the viscera were 60% (63/105) at 15°C, 1.64% (3/183) at 12°C, 1.16% (2/172) at 9°C and 1.42% (2/141) in the control group (7.5°C). The free larvae showed no, slight and strong movement at 9°C, 12°C and 15°C, respectively, in saline water, and the larvae were present in a coiled state at 9°C and 12°C. The results suggest that temperature plays an important role in the intra-vitam larval migration of A. pegreffii. In addition, the free larval behavior of A. pegreffii in vitro increased with increase in temperature. Therefore, to prevent human anisakiasis caused by the consumption of raw fish, it is important that the temperature of the aquarium must be maintained below 12°C, which is the critical temperature zone.
        4,000원
        72.
        2020.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        농촌진흥청 국립원예특작과학원에서는 2017년에 절화 수명이 길고 수량이 많은 연한 핑크색의 스프레이 장미 ‘Pink Shine’ 을 육성하였다. 모본은 ‘Fire Flash’로 붉은 복색의 스프레이 장미이며, 부본은 ‘Pink Charm’으로 핑크색이며 흰가루병에 강하다. 이 두 품종을 2012년 인공교배하여 이듬해인 2013년 1월에 파종, 9cm 포트 묘에 정식하여 관능 평가 실시 후 도태시켜 39개체 의 실생을 얻었다. 이후 화형, 화색, 꽃잎 수, 절화수량, 병 저항성 등을 고려하여 2015년까지 5개체를 선발하여 유사 품종인 ‘Missha’를 대조로 하여 2017년까지 3차에 걸친 특성 검정을 실시하였다. 그 결과 가장 우수한 ‘원교 D1-325’를 최종선발하여 ‘Pink Shine’으로 명명 후 2018년 3월 22일 품종보호출원(제 2018-212호)하여 2019년 6월 21일에 품종보호권(제7786호)이 등록되었다. 화색은 연한 핑크색(RHS, R36D)이며 잎의 색은 녹색(RHS, G137A)으로 대조 품종 ‘Missha’와 동일하였다. 꽃잎 수는 67.8개, 화폭 5.4cm, 화고 3.2cm로 ‘Missha’보다 컸으며 평방미터당 연간 절화수량은 131본, 절화수명은 15.3일로 ‘Missha’ 보다 우수하였다.
        4,000원
        74.
        2020.08 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        The Korea Astronomy and Space Science Institute (KASI) has been developing a next-generation coronagraph (NGC) in cooperation with NASA to measure the coronal electron density, temperature, and speed simultaneously, using four different optical filters around 400 nm. KASI organized an expedition to demonstrate the coronagraph measurement scheme and the instrumental technology during the 2017 total solar eclipse (TSE) across the USA. The observation site was in Jackson Hole, Wyoming, USA. We built an eclipse observation system, the Diagnostic Coronal Experiment (DICE), composed of two identical telescopes to improve the signal-to-noise ratio. The observation was conducted at four wavelengths and three linear polarization directions in the limited total eclipse time of about 140 seconds. We successfully obtained polarization data for the corona but we were not able to obtain information on the coronal electron temperature and speed due to the low signal-to-noise ratio of the optical system and strong emission from prominences located at the western limb. In this study, we report the development of DICE and the observation results from the eclipse expedition. TSE observation and analysis with our self-developed instrument showed that a coronagraph needs to be designed carefully to achieve its scientific purpose. We gained valuable experience for future follow-up NASA-KASI joint missions: the Balloon-borne Investigation of the Temperature and Speed of Electrons in the Corona (BITSE) and the COronal Diagnostic EXperiment (CODEX).
        4,300원
        76.
        2020.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Enterotoxigenic Escherichia coli는 신생 및 이유기 돼지 설사의 주요 원인체로서 전세계적으로 양돈산업에 큰 경제적 손실을 끼치고 있다. 그러나 현재 국내에는 이러한 E. coli가 보유하는 다양한 병원성유전자의 분포 및 특성에 대한 정보가 부족한 실정이다. 이에 본 연구에서는 2013년부터 2016년까지 국내 163개 양돈농장에서 이유기 설사증 개체로부터 면봉스왑 샘플을 채취하여 동일 농장의 개체일 경우 5개에서 10개 정도를 혼합한 후, MacConkey agar에 배양하여 최종 API 32E system을 통하여 동정하였다. 분리된 모든 균주에 대해서 3가지의 다른 multiplex PCR을 수행하여 총 13종의 병원성유전자의 분포를 확인하였다. 이를 통하여 총 172개의 최소 한가지 이상의 병원성 유전자를 가지는 E. coli 균주를 확인하였고, 그 결과 병원성 유전자의 분포는 (1) fimbrial adhesins (43.0%): F4 (16.9%), F5 (4.1%), F6 (1.7%), F18 (21.5%), and F41 (3.5%); (2) toxins (90.1%): LT (19.2%), STa (20.9%), STb (25.6%), Stx2e (15.1%), EAST1 (48.3%); and (3) nonfimbrial adhesin (19.6%): EAE (14.0%), AIDA-1 (11.6%) and PAA (8.7%)로 나타났다. 결론적으로 본 연구결과는 국내 양돈농장의 이유기 설사증에 관연하는 E. coli는 다양한 종류의 병원성 유전자를 가지고 있으며 그러한 병원성 유전자의 조합도 매우 다양하게 분포하고 있음을 나타낸다.
        4,000원
        77.
        2020.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Porcine epidemic diarrhea virus (PEDV) is a porcine coronavirus that causes enteric diseases characterized by watery diarrhea and dehydration in suckling piglets. Concentrated and highly purified viruses are required for the preparation of vaccines, diagnostics, and virus research. Currently, most protocols for virus purification require ultracentrifugation, which can be an instrumental barrier to routine operations in a laboratory. In this study, the efficacy of low-speed centrifugation for virus concentration was examined. The SM98 strain of PEDV was propagated in Vero cells and pelleted by centrifugation for 3 h at high speed (100,000 × g) or for 18 h at low speed (10,000 × g). The efficacy of virus concentration was analyzed by virus titration and western blotting. The amounts of infectious viruses and viral proteins in the pelleted samples obtained by low-speed centrifugation were comparable to those obtained by high-speed centrifugation. Interestingly, the pelleted sample impurity level was lower in low-speed than in high-speed centrifugation. In summary, we describe an efficient, easy-to-perform protocol for the preparation of purified and concentrated PEDV.
        4,000원
        78.
        2019.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        국립원예특작과학원에서는 2015년 거베라 신품종 ‘Red Velvet’ 을 육성하였다. ‘Red Velvet’은 2007년 10월부터 2008년 2월까지 주홍색 반겹꽃 스탠다드 거베라 ‘Rose’와 적색 반겹꽃 ‘Sabana’를 각각 모본과 부본으로 인공교배하여 얻은 187개체의 교배실생 분리집단으로부터 2008년부터 2015년까지 8년 동안의 개화 및 생육특성검정을 거쳐 선발된 절화용 스탠다드 거베라 품종이다. ‘Red Velvet’은 초세 강건한 갈색 화심의 적색(RHS R45B) 반겹꽃 대륜계통이다. 꽃의 평균 직경은 12.6±0.5cm이고, 내부설상화 및 통상화의 직경은 각각 5.7±0.3cm와 2.8±0.2cm이다. 꽃대는 꽃목직경이 상부 5.4±0.4mm, 중부 7.8±0.7mm 정도로 굵으며, 화경장은 60.0±8.1cm로 길다. 절화수명은 9.8±2.3일로 대조품종과 비슷하며, 연간 채화량은 48.3±4.8본/주로 많은 다수성 품종이다.
        4,000원
        79.
        2019.12 KCI 등재 구독 인증기관·개인회원 무료
        해충의 예찰 없이 작물 정식 초기에 천적을 먼저 적용할 수 있는 천적-서식처 혼합적용(Natural Enemy in First; NEF)기법의 방제효과를 확인하였다. 시설 딸기에서 조사 12주차에 NEF처리구와 천적 단독 처리구에서 친환경자재 처리구 대비 각 83%, 70%의 점박이응애 밀도 억제효과를 확인할 수 있었다. 또한 예찰 없이 작물 정식 초기에 NEF를 적용한 처리구에서 천적 단독 처리구보다 3배 이상의 높은 천적 밀도를 확인하였다.
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