Background : This experiment was carried out to improve the labor-intensive task to construct of the traditional ginseng shading system in Korea. In this experiment the selected shading materials was new 6 kinds that HDPE - knitted- monofilament and taped PE Net, with taking into account of the amount of light and the amount of leakage to screen the UV stabilized 0.3% - 0.6%. Compared with the traditional shading material, the black-shading material (wt. 200g/㎡) and the yellow shading material (wt. 300g/㎡) was suitable for the ginseng growing environments in Korea. Methods and Results : The installation of shading system was the 45 degree slope -2 meter height from the ground with the selected shading materials for the wide structure ginseng cultivation. Temperature and light intensity was measured with a week interval from the surface to a height of 30cm. The two kinds of the 1st selected materials and the traditional black + blue two layer shading materials, aluminum screen, Canada Gintec shading material, total 5 kinds of materials were installed in wide structure. The growing environmental analysis was measured using data logs (watchdog 1600series). Conclusion : In the first selection of shading materials for Ginseng wide structure, the levels of light intensity were higher in order of blue-shading> Aluminum- shading> black-shading order. The temperatures of the under wide structures were lower in order of the black-shading< aluminum-shading screen< blue-shading order, especially the temperature of the black shading material was lower than the outside temperature. For the second selection of shading material of the ginseng wide structure, the black and yellow shading material were superior to the other materials, in the amount of light shading material and growing conditions and the degree of leakage etc. The temperatures of mid-May in wide structures of ginseng cultivation were higher in order of aluminum-screen> Canada shading materials (Gintec Co.) 2 layer material> Yellow shading> black shading order, and also the amounts of light intensity also was shown to be with the same manner

Background : The production of healthy ginseng seedling is a critical part of growing 5-6-year-old fresh ginseng. Recently, for the problem of replant failure, it becomes more and more difficult to find first planted field for ginseng seedling cultivation. In this situation, This study was conducted to examine the possibility of seedling production practices of ginseng by utilizing the multi-beds cultivation system as a way to produce ginseng seedling stably. Methods and Results : This experiment was conducted by installing a 3-layed cultivation beds using 50㎜ sandwich panels. As the 1st and 2nd beds get insufficient amount of light, fluorescent lights were installed in 2 rows to further lighten up. And the 3rd bed used natural light. The bed soil used was formulated by mixing saprolite, yakto and peat moss. The control was cultivated under natural light on 1-layed bed with commercial bed soil for ginseng seedling. The commercial bed soil had pH 7.24, higher than the standard of permission, 6.5; and its calcium and sodium contents were 10.7, and 0.85 cmol+/㎏, respectively, higher than the standard. The available phosphate concentration was 34.0㎎/㎏, lower than the appropriate level of 100 ㎎/㎏. The mixing bed soil also had pH 7.69, higher than the standard but its nitrate and phosphate were within the appropriate range. The intra-facility light quantities of the 1st and 2nd beds were constant at around 100 μmol during days. But the light quantity of the 3rd bed was 51.4 μmol in average daily value. The roots of the 1st-3rd beds were found to weigh 0.58 – 0.73 g while those of the control group, about 0.47g to show heavier root weight of the multi-beds cultivation ginseng than that of the control. As for the yield, the control showed 145 ㎏/10a whereas the 1st-3rd beds showed higher levels between 183 – 228 ㎏/10a. Conclusion : In the multi-beds cultivation of ginseng seedling, fluorescent lamps seem to have provided sufficient light as an artificial light source. The soil for ginseng seedling production is different from ginseng cultivation field soil, additional study will be also necessary to set up the physiochemical range of bed soil for ginseng seedling.

Background : Ginseng farmers prefer first planted field because of replant failure. But the accumulation of nutrients is higher in the first planted field due to using large amounts of chemical fertilizers and manure. The higher phosphate concentration in preparation field decreased emergence ratio and occurred leaf chlorosis in ginseng. The purpose of this study was to develop a technology for reducing phosphate concentration in preparation field of ginseng. Methods and Results : This study was performed in farm's fild located Yeongju in 2015. Treatment of plots consisted of sudangrass, maize, deep plowing + sudangrass and microbes(Rhodobacter sphaeroides, Bacillus subtilis, streptomyces griseus) + sudangrass. sudangrass and maize were sown in early May to 5kg / 10a. Microbes diluted 500 times were treated to drench the soil before sowing crops(400L/10a). The main survey was yield of crop, soil chemical properties, reduction ratio of phosphate and changes in available phosphate. Reduction ratio of phosphate in the plot of maize and Rhodobacter sphaeroides were 27.9% and 24.3%, respectively, which were higher 2∼5% than the control plot. Fresh weight yield was the highest with 11.1ton / 10a in the plot of maize and ratio of dry matter was the highest with 27% in the plot of maize. Conclusion : The phosphate removal ability of maize was better than sudangrass in phosphate accumulation soil. Subsoil reversed using excavators used by some farmers could not mitigate of phosphate and salts concentration in preparation field. The soil that phosphate concentration is more than 1,000mg/kg is required a long-term management.

Background : The Ginseng has been one of the crops that represents Korea, and the main export items in the 1960s, Now it occupies the first exports of agricultural products. The Eco-friendly cultivation of Ginseng was difficult for various reasons. But the cultivation in Green House can be suppressed disease occurrence, when utilizing an environmentally friendly materials has the advantage that can dramatically increase the income by possible organic production. For other crops, it made up a lot of studies on water management, But the studies on water management of ginseng is somewhat lacking. Therefore, this study was conducted to establish appropriate soil- moisture-management-systems within the house of ginseng cultivation. Methods and Results : The type of house is a double steel structure. The first layer was PE film, the second layer was the blue shading net and 75% black shading net. The Soil texture was silt loam and the Soil Series was YEONGOK Series. Install the house on the adjacent paddy soil and upland soil, and planting on April 30, 2011 which was cultivated by the straw mulching. The irrigation start point was the 40, 30, 20kpa, and the end point was treated with 15, 10kpa. Soil moisture measurements were determined by tension meter(Jet Fill) depth 20cm in soil. Growth and Yield Survey was conducted in accordance with the Rural Development Administration (RDA) standard research. Statistical analysis of the results were analyzed using the Microsoft's Excel 2003. Conclusion : In a survey due to differences in soil traits and Irrigation point, from 2 years to 4years of root weight and yield of Ginseng were heavier in the upland, especially 30kpa irrigation point treatment was heaviest than the other treatments. From 5year to 6 Years of ginseng has showed heavier in paddy soil, the most treatment was 30kpa- 15kpa irrigation point. This trend was the same in upland soil. Soil moisture management in the case of silt loam, the humid-state-control was more advantageous than dry-state control in upland and paddy soil.

Geneally, rice seeds regardless indica or japonica are showing low germination ratio or completely lost germination ability together with lost of good eating quality under high temperature and humidity conditions. Thus, this study was designed to evaluate a longevity for conservation of good eating quality during long term storage in rice. For the longevity evaluation, germination ability was studied after 5 days of high temperature and humidity stress (50℃/RH 95%). Dharial, originated from Bangladesh and showing weedy type with red pericarp, was selected as a good donor for longevity genes. A mutant was developed from Dharial through EMS mutagenesis and two populations of Dharial/4*Ilmibyeo and Dharial/4*Gopumbyeo were also developed for genetic study. In the 2-DE analysis followed by MALDI-TOF MS with wild and mutant lines, several candidate genes were identified. In the longevity test of two populations, a few lines showing good germination ability after high temperature and humidity stress were selected and subjected to confirm the relationships between longevity and conservation of good eating quality under long term storage.

An unbalance of rice productions and consumptions caused serious problems in both of agricultural area and grain market of korea. In recently, various efforts for rice processing products such as rice noodles and rice wines are in progress to overcome the unstable rice market. Among them, waxy rice is predominant items in processing rice. However, varietal features of starch viscosity are not considered in the processing industries and rice breeding field. In this study, 12 waxy rices and 2 cultivars Ilmibyeo(japonica) and IR72(Indica) were studied for physicochemical and amylogram to characterize the use of waxy rices. The amylose contents of waxy rices were from 7.1 to 8.1% with soft gel consistency and relatively low alkali digestion value(1.4% KOH) compare to Ilmibyeo. In the amylogram analysis(RVU) of waxy rices, unlikely normal rices, very fast peak time(about 3.5 min.) was obtained compare to that of 6.2 min. of Ilmibyeo. And 2 to 3 groups were classified based on peak viscosity and consitency of RVU. Wangchal, Odorokimochi and Hangangchal showed relatively high peak viscosity seemed not suitable for rice cake due to the hardness speed. And most of waxy rices developed in korea were believed to be suitable for oil fried cakes(Hankwa) because of a certain grade of starch degradation speed. And Mochiminori and Midoromochi originated from japan could be a good sources in waxy rice breeding program to improve the cooking properties especially in slow down of hardness speed with very low peak viscosity, hot viscosity and cool viscosity.

The removal of nitrogen compounds from a wastewater is essential and it is often accomplished by biological process. An aerobic nitrate-removing bacterium was isolated from a municipal sewage treatment plant and soil. On the basis of its morphological, cultural and physiological characteristics and 16S rRNA sequencing data, this strain was identified as Pseudomonas fluorescens, and named as P. fluorescens K4. The optimal conditions of the initial pH and temperature of media for its growth were 7.0~8.0 and 30℃, respectively. P. fluorescens K4 was able to remove 99.9% of nitrate after 24 h in a culture. The strain could grow with a nitrate concentration up to 800 mg/l and was able to remove 99.9% of nitrate after 104 h of incubation. The optimal electron donor was sodium citrate for a nitrate removal. The strain K4 showed a capability of a complete nitrate removal when the initial C/N ratio was 1.0. An effect of the initial seed concentration was observed for a cell of 10% (v/v) for a nitrate removal. Especially P. fluorescens K4 could completely remove 200 mg/l ammonium for 3 days.

Growth and quality of rice are affected by various factors including the location of cultivation. This study was conducted to investigate the effects of altitudinal locations on the growth and yield-related components of rice. Nineteen Japonica type varieties were grown at Iksan (altitude, 10 m), Imsil (altitude, 150 m), Jinan (altitude, 275 m) and Jangsu (altitude, 430 m) at a similar latitude in Jeonbuk province in the south western Korea. Minimum air temperature showed a strong negative correlation with altitude. The morphological traits and yield-and quality-related components were analyzed. Longer days to heading was required at higher altitudes. However, culm length, panicle length, panicle number, grain number and rice yield were reduced at higher altitudes. Protein content of brown rice increased but fatty acid content decreased at higher altitudes. Amylose content was affected by neither the altitude nor the ecotype. Palatability of polished rice tends to be improved at higher altitudes and in early-maturing ecotypes but its relationships with altitude and ecotype were not significant. Head rice ratio was lower at higher altitudes but broken rice ratio vise versa. These results indicate that growth and quality of rice are affected significantly by changes in temperatures at the locations of different altitude. Also, the characters related to yield and quality of rice often respond incompatibly to the changes in altitudes. These results could provide valuable information for the strategic planning of rice production in geographically diverse areas

Thirty-nine out of eighty-five barley varieties/strains survived until heading stage in the saline experimental field (0.03-0.05~%~;salt) and they were used for pollen study. Light and scanning electron microscopic observations revealed two distinctive types of barley pollens: one transparent and small in size and the other dark and larger. In addition, both types of pollens were stained with Alexander's stain and it was found that the smaller and transparent pollen was cytoplasm-devoid (CD) while the larger pollen was cytoplasm-rich (CR). Sixteen out of 39 barley varieties/lines grown in the saline soil had CR pollens, which were rarely observed in the barley plants grown in the non-saline soil. Moreover, it was observed that salt stress severely reduced seed setting in the varieties having degenerated pollens. These results suggest that salt stress affects the fertility of barley pollen. The sterile pollen was undersized and lack of cytoplasm probably due to abortion. Furthermore, a varietal difference existed in the response of pollen development to salt stress.

This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress (13~circC) in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -sus­ceptible varieties.

Gahwang is an excellent chipping cultivar with yellow flesh, short oval tuber shape and mid-early maturity. Its yields were a little higher than Atlantic through the regional trials with five locations. It has a lower incidence of internal brown spot comp

Shinnamjag is a round, smooth, white-skinned, and fleshed potato cultivar with erect plant shape and early maturity. It has excellent table quality. Its yields are high, especially with prominent marketable yield compared to Superior, a leading potato cul