Background: Porcine pluripotent stem cells (pPSCs) would provide enormous potential for agriculture and biomedicine. However, authentic pPSCs have not established yet because standards for pPSCs-specific markers and culture conditions are not clear. Therefore, the present study reports comparative pluripotency characteristics in porcine induced pluripotent stem cells (piPSCs) derived from different viral transduction and reprogramming factors [Lenti-iPSCs (OSKM), Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM)]. Methods: Porcine fibroblasts were induced into Lenti-iPSCs (OSKM) and Lenti-iPSCs (OSKMNL) by using Lentiviral vector and Sev-iPSCs (OSKM) by using Sendaiviral vector. Expressions of endogenous or exogenous pluripotency-associated genes, surface marker and in vitro differentiation in between Lenti-piPSCs (OSKM), Lenti-iPSCs (OSKMNL) and Sev-piPSCs (OSKM) were compared. Results: Colonial morphology of Lenti-iPSCs (OSKMNL) closely resembles the naïve mouse embryonic stem cells colony for culture, whereas Sev-iPSCs (OSKM) colony is similar to the primed hESCs. Also, the activity of AP shows a distinct different in piPSCs (AP-positive (+) Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM), but AP-negative (-) LentiiPSCs (OSKM)). mRNAs expression of several marker genes (OCT-3/4, NANOG and SOX2) for pluripotency was increased in Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM), but Sev-iPSCs (OSKM). Interestingly, SSEA-1 of surface markers was expressed only in Sev-iPSCs (OSKM), whereas SSEA-4, Tra-1-60 and Tra-1-81 were positively expressed in Lenti-iPSCs (OSKMNL). Exogenous reprogramming factors continuously expressed in Lenti-iPSCs (OSKMNL) for passage 20, whereas Sev-iPSCs (OSKM) did not express any exogenous transcription factors. Finally, only Lenti-iPSCs (OSKMNL) express the three germ layers and primordial germ cells markers in aggregated EBs. Conclusions: These results indicate that the viral transduction system of reprograming factors into porcine differentiated cells display different pluripotency characteristics in piPSCs.

Aligned with the recent shift in fruit consumption trends, there is a growing increase in the cultivation of these fruits. Thus, various types of small fruits, including blueberries, have recently been introduced and cultivated in Korea. According to the data, there has been a notable uptick in blueberry farming, in stark contrast to the decline in cultivation of black raspberries, black chokeberries, and blackcurrants. New varieties of blueberries continue to be introduced and cultivated, aligning with the recent consumption trends and are expected to be consistently cultivated in the future. Despite the decrease in the other berries, health benefits associated with all berries have ignited growing consumer interest, resulting in domestic and international market expansion. In conclusion, this research underscores the importance of identifying plant varieties that are well-suited to Korea's climatic conditions, validating efficient cultivation strategies, and establishing robust distribution networks to foster sustainable development in the berry industry.

󰡔유몽천자(牖蒙千字)󰡕는 한국개신교선교사 게일(James Scarth Gale(한국명 : 奇一), 1863-1937)과 그의 조사(pundit) 이창직(李昌稙, 1866-1936)이 함께 편찬한 경신 학교와 정신여학교의 교과서이다. 이 책은 총 4권으로 구성된 교재다. 여기에 실린 내용 은 자연과학, 사회과학, 서양의 역사, 서양의 인물, 서양의 문학, 우리의 한문고전에 관한 것 등으로 이루어져 있다. 더불어 개화기에 우리의 어문 생활에 필요하다고 생각한 한자 와 관련 정보, 한자어, 국한문, 한문에 관한 지식을 정식 학교 교육을 받는 학생들에게 알 려 주기 위하여 편찬한 것이다. 본고에서는 이러한 󰡔유몽천자󰡕 전집의 체계와 구성, 전 집을 구성하는 3가지 문체 유형, 그리고 전집에 새겨져 있는 개신교선교사의 문체실험의 역사를 고찰했다.

Zhong Hua Zi Jing is Chinese teaching material promoted nationwide and overseas for the purpose of “fast literacy and early reading”, which covers Level 4 Chinese characters specified in the HSK exam outline. Its content includes knowledge of fifty major categories, including astronomy, geography, human relations, history, and the like. Therefore, this study is based on Xu Tongqiang’s sinogram-based theory. This paper proposes a teaching plan using the Zhong Hua Zi Jing in teaching Chinese as a foreign language in order to achieves positive results in vocabulary and Chinese character learning. A survey was first conducted on Korean students in years three and four of the Chinese language major to demonstrate their level of understanding of Chinese characters included in Zhong Hua Zi Jing, and to elucidate the level of understanding of Chinese characters Zhong Hua Zi Jing. Then, the necessity and specific solutions of using Zhong Hua Zi Jing to teach vocabulary mainly based on Chinese characters was explored.

This study was conducted to reset the maximum residue limit (MRL) for didecyldimethylammonium chloride (DDAC) in broiler chickens. The disinfectant containing DDAC (10%, w/w) was diluted 160 times and evenly sprayed on the bodies of twenty-four broiler chickens at a rate of 15 mL per day per bird for 7 days. After the disinfectant treatment, tissue samples were collected from six broiler chickens at 0.25, 1, 3 and 5 days, respectively. Residual DDAC concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 2.0~10.0 μg/kg), recoveries (86.9~118.6%), and coefficient of variations (<19.98%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In all tissues except for liver, DDAC was detected more than LOQ at 5 days after the disinfectant treatment. In liver tissues, DDAC was detected more than LOQ at 3 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal period of DDAC in poultry tissues was established to 26 days. In conclusion, the developed analytical method is sensitive and reliable for detecting DDAC in poultry tissues. When DDAC disinfectant is sprayed on a poultry house in the presence of broiler chickens, it is necessary to keep the disinfectant from contacting the body of the livestock.

This study was conducted to develop a seed propagation protocol of Hemerocallis taxa native to the Korean Peninsula. In this study, the seed dormancy types were classified. The germination traits of H. thunbergii, H. dumortieri, H. hakuunensis, and H. hongdoensis were also characterized. The initial embryo to seed length ratio (E:S ratio) in seeds of the four Hemerocallis species was between 0.80 and 0.90 at harvest, however, the E:S ratio did not increase until just before germination. Thus, seeds of the four Hemerocallis species had no morphological dormancy (MD), and at least 84% of seeds of all tested species germinated at 25/15℃ under light within 30 days. The lower the incubation temperature, the lower the germination rate of seeds of the four Hemerocallis species. However, the temperature range for germination was expanded due to old stratification. Gibberellin treatment increased the germination rate or germination speed at a relatively low temperature of 15/6℃. However, this treatment did not improve the germination rate as much as the temperature at 25/15℃. Under 15/5℃ of incubation, cold stratification increased the final germination percentage and rate/speed and expanded the temperature range for germination from high to low (25/15℃ → 15/6℃). This indicates that the seeds have a conditional dormancy (CD) that germinates in a narrow temperature range. In conclusions, the four Hemerocallis seeds exhibited nondeep physiological dormancy.

This study evaluated the fungicidal efficacy of weakly acidic hypochlorous acid water (WAHW) against Microsporum canis (M. canis) and its therapeutic effect on M. canis-infected mouse skin. WAHW was produced by a WAHW generation module. A fungicidal efficacy test by the broth dilution method was used to determine the lowest effective concentration of the WAHW. The lowest effective concentration of WAHW was less than 10 ppm. For T-1, T-2, and T-3, 30 ppm of WAHW was applied to the infected skin once, twice, and three times a day, respectively, and for T-4, 50 ppm of WAHW was applied once a day. On the 3rd day after the initiation of treatment, skin scores in all of the WAHW-treated groups were significantly decreased compared to those in the positive control group (PC) (p<0.05), and there were no significant differences compared to the normal control (NC). The area of the infected skin in all of the WAHW-treated groups was significantly decreased compared to PC from the first day after the initiation of WAHW treatment (p<0.05). The results showed that WAHW had a fungicidal efficacy on M. canis at less than 10 ppm, and it was effective in improving skin symptoms when 30 ppm of WAHW was applied to the M. canis-infected area once a day for five days or 50 ppm of WAHW was applied once a day.

본 논문에서는 대규모 실시간 매칭의 생존 게임에서 플레이를 위한 유저들의 소셜 관계에 대해 연구한다. 특 히 “사전 팀 구성”을 통한 자의적인 팀 구성이 어떤 방식으로 유저들을 연결하는 지 연구하고자 한다. 다수 의 사람 간 집단 역학에서 나타나는 특성이나 패턴에 대한 조사를 중심으로 하였으며, 개인의 특성은 보조적 인 수단으로만 사용된다. 이번 연구에서는 게임을 플레이하는 유저들의 익명화 된 대규모 데이터를 활용하며 이에 대한 간소화된 집계 방법을 제안한다. 데이터 세트에는 사전 팀 구성에 관한 11,259만 줄의 속성이 포 함되어 있으며, 데이터에서 우리는 250만개의 노드와 1,182만개의 무방향 에지가 있는 협업 네트워크를 구성 하여 대규모 게임 내 협동 네트워크를 만듭니다. 연결 정도, 경로 길이, 클러스터링 및 소속 하위 컴포넌트의 크기 등 네트워크에 관한 수치를 통해 게임내 소셜 활동에 대한 이해를 높이고자 한다. 본 논문에서는 다음 의 두가지 특성을 중심으로 결론을 제시한다. 첫째, 네트워크 내에는 대규모로 연결된 2개(전체의 44% 및 2%)와 나머지의 파편화된 하위 컴포넌트로 구성 되어있다. 이 대규모 컴포넌트 중 작은 쪽은 한국 유저로만 구성되어 있다. 둘째, 컴포넌트 크기 별 평균 연결 거리와 군집화 계수, k-core를 확인함으로써 기타 다른 네 트워크 대비 이웃 간 연결이 강하면서 전체적으로는 비교적 멀리 떨어져 있음을 확인한다.

자기공명(magnetic resonance, MR)영상에서 주로 발생하는 Rician 노이즈는 영상의 화질을 저하하는 주요 요소 중의 하나이다. 본 연구에서는 노이즈 제거에 효율적이라고 잘 알려진 총변이(total variation, TV) 알고리즘을 모 델링하여 Rician 노이즈 레벨에 따른 파라미터를 최적화하고자 한다. 시스템은 8채널 기반의 3.0 T 장치를 활용하였 고 물 팬텀 영상을 획득하여 각각 Rician 노이즈를 0.05, 0.10, 0.15, 그리고 0.20 값을 부가하였다. TV 알고리즘 은 Rudin-Osher-Fatemi 모델을 기반으로 모델링하였고 최적화를 수행하기 위하여 반복수 파라미터를 조정하여 획득된 영상에 적용하였다. 결과적으로 Rician 노이즈 레벨을 0.05, 0.10, 0.15, 그리고 0.20을 사용하였을 때 각 각 30, 40, 80, 그리고 120 반복수를 기반으로 한 TV 노이즈 알고리즘에서 가장 우수한 신호 대 잡음비(signal to noise ratio, SNR)와 대조도 대 잡음비(contrast to noise ratio, CNR) 결괏값이 도출되었다. 또한, 최적화된 반복수를 적용한 TV 알고리즘을 사용한 MR 영상에서 기존의 위너 및 중간값 필터를 사용하였을 때 비하여 SNR과 CNR 모두 우수한 값을 획득할 수 있었다. 특히 기본적으로 획득된 MR 영상보다 최적화된 TV 알고리즘을 적용한 영상의 평균 SNR과 CNR은 각각 3.11 및 3.31배 향상됨이 증명되었다. 결론적으로, 노이즈 제거 효율이 우수한 TV 알고리즘의 최적화된 파라미터를 활용한다면 MR 영상에서의 활용 가능성이 클 것으로 기대한다.

PURPOSES : The current research aims to evaluate the impact of coating materials and temperature on the percentage of bead loss in pavement markings. METHODS : Five mixtures with varying numbers of coating layers (C0, C1, C2, C3, and C4) were prepared to assess the effect of coating layers on bead loss. The effect of stripping was simulated using a modified Hamburg Wheel Tracking test. Furthermore, the influence of temperature and coating material on bead loss was examined using control mixture (without coating), YR, and SY coating mixtures. The percentage bead loss was evaluated by a developed image analysis program. RESULTS : The results demonstrated a substantial reduction in bead loss as the number of coating layers increased, with the C4 mixture showing an impressive 4.3% bead loss after 500 HWT braking cycles compared to 27.4% for the C0 mixture. Higher testing temperatures resulted in increased bead loss, with the control mixture exhibiting the highest percentage loss at 7,500 HWT rolling cycles. Conversely, the YR and SY coating mixtures displayed superior resistance to bead loss. Statistical analysis confirmed the significance of coating layers in reducing bead loss, further supporting the effectiveness of coatings in preserving bead adhesion during HWT cycles. CONCLUSIONS : The findings highlight the potential of coating materials as a key protective measure for enhancing the longevity and performance of pavement markings.

‘지역사회 계속거주(AIP: Aging in Place)’는 자율적이고 독립적인 노년의 삶을 원하는 노인들의 욕구 와 노인시설 운영비용 부담을 줄이고자 하는 정부의 필요성이 부합되면서 인구 고령화에 대처하는 새로운 패러다임으로서 주목받으며 활발히 논의되고 있다. AIP에 관한 국내외의 다양한 연구들은 AIP가 노년기의 삶의 질에 긍정적인 영향을 미치고 있음을 주장하는데, 이는 노인이 자립적으로 생활할 수 있는 사회환경이 제공되었을 때 가능하다. 본 연구는 사람이 살아가는데 가장 기본적인 필수 의료와 임종기 완화의료 및 돌봄 등의 실태를 점검하고 AIP 관련 정책의 현행 법령과 제도를 살펴봄으로써, 국가와 지방정부의 책무는 무엇이며 향후 그 책무를 충실히 이행하기 위한 실효적 방안들은 무엇인지에 관하여 제언한다.