The high quality of gene set is necessary to study the functional research of genes. Although perilla is cultivated as an oil crop and as a vegetable crop in Asian countries such as Korea, Japan, northeast China and Nepal, the reference genome is absent. To assembly perilla gene set, we sequenced the various tissues of perilla (Perilla citriodora) RNA-seq with Illumina HiSeq platform, generating 548,549,314 short reads. When de novo transcriptome assembly was performed with five samples, 86,396 and 38,413 transcripts were assembled as total and representative transcripts, respectively. Using 1,917,424 proteins at Phytozome ver. 9.1, we annotated the perilla assembled transcripts, and 66,139(76.55%) and 24,030(62.55%) transcripts showed the similarity with known plant proteins (E-value < 1e-10) as total and representative transcripts, respectively. Among the diverse molecular functions, we were interested in the regulatory components, such as transcription factor and transcription regulator. Using this data, we identified 499 transcripts annotated the putative transcription factor differentially expressed transcripts. 165 putative transcription factors were significantly expressed in perilla flower and 121 putative transcription factors in both leaf and flower. This study provides the perilla reference gene set and the understanding of the molecular regulation of transcription factor dependent on the tissue.

라돈은 우라늄-238과 토륨-232가 방사성붕괴 과정을 거친 후 생성되며, 무색, 무취의 불활성 기체로서 지하 또는 밀폐된 공간에 축적된다. 우라늄-238과 토륨-232는 지각의 암석이나 토양 등에 포함 돼 있다. 건축자재는 암석이나 토양을 재료로하여 만들어 진다. 가스 형태의 라돈은 호흡기를 통해 폐로 유입되고 라돈의 딸핵종이 폐나 기관지에 침적 되어 폐암을 일으키는 원인이 된다. 본 연구는 광주광역시 광산구에 위치한 신축 아파트를 대상으로 창문을 닫고 열은 상태에서 라돈 측정기를 이용하여 측정하였다. 측정 결과로 보아 신축 아파트 실내 평균 라돈농도는 미국 일반인 공기 중 라돈가스 최대허용농도 기준치 4 pCi보다 이하의 값이 나타난다는 것을 볼 수 있다. 측정 결과로 볼 때 신축 아파트의 라돈농도로 인한 피폭은 크지 않을 것으로 예상한다. 그러나 라돈가스가 신체 내에 축적이 되면 폐와 같은 경우는 폐암과 같은 피폭에 의한 피해를 얻을 수 있으므로 방사선 방어적 측면에서 측정 결과와 같이 라돈 농도를 낮추기 위해 창문을 자주 열어 환기를 시켜 피폭을 줄이는 것이 필요하다고 생각 된다.

Cholesterol embolization syndrome (CES) is a multiple systemic disease caused by the embolization of cholesterol crystals from an atherosclerotic plaque of a proximal large-caliber artery, which results in the occlusion of distal small to mediumsized arteries. CES is characterized by development of a multitude of small emboli over time, and should be distinguished from arterial thromboembolism, which occurs through the obstruction of medium-sized to large arteries by one or a few large emboli. We report on a case of CES initially presenting as acute limb ischemia following an intervention for iliac artery occlusion.

The isolated single coronary artery is a rare congenital anomaly, in which both coronary arteries arise from a solitary ostium. Diagnosis of coronary anomalies and identification of the exact anatomy of coronary arteries has significant clinical importance, hence, myocardial ischemia or sudden cardiac death is usually related to its course of anomalous coronary artery. Most patients with a single coronary artery are asymptomatic and have normal electrocardiogram and negative stress tests. However, if the patient has other structural abnormalities, for example, ventricular hypertrophy, the exam is determined. This report describes a case of single coronary artery, where the right coronary artery originated from the distal left circumflex artery in a patient with hypertrophic ardiomyopathy.

Rice is one of the most important cereal crops in the world and a model plant for functional genomics of monocotyledon. Recently, rice crop loss is estimated to be approximately 30% of the total yield due to herbivorous pests, mainly insects. Cry1Ac toxin is a protein produced by the bacterium Bacillus thuringiensis and has insecticidal properties. CryBP1 toxin also is an insecticidal protein produced by the bacterium Bacillus popilliae. These two toxic genes derived bacteria, which were inserted into a binary vector, have been introduced into rice plants by Agrobacterium tumefaciens mediated transformation in order to enhance resistance to insects. Here, we utilized anthers to regenerate transgenic rice plants when it has been plated on the callus induction media as a callus-inducing material. Anther culture has a benefit in terms of being apt to produce doubled haploids in short term in plants breeding compared to seed culture. However, anther culture method in generating transgenic rice still has low productivity of plant regeneration in some genotypes of Japonica rice. Therefore, we examined the efficiency of callus induction and transformation with three different cultivars of Japonica rice, Haiami, Ungwang and Dongjin. In this study, our results showed that Haiami is the best genotype among three cultivars of Japonica rice as callus inducing material in anther culture to produce transgenic rice plants conferring resistance to insects.

Neutropenic enterocolitis (NE), the most serious gastrointestinal complication, has been reported as a clinical syndrome that occurs in the setting of disease- or chemotherapy-induced neutropenia. Complications of NE include bowel necrosis with perforation, fistula, stenosis, massive bleeding, abscess formation, and pneumatosis intestinalis (PI). Most physicians recommend initial conservative management with bowel rest, intravenous fluids, total parenteral nutrition, broad-spectrum antibiotics, and normalization of neutrophil counts. Surgical intervention is recommended in the event of obstruction, perforation, persistent gastrointestinal bleeding despite correction of thrombocytopenia and coagulopathy, or clinical deterioration. We experienced a patient whose abdominal computed tomography scan showed pneumoretroperitoneum, intramural gas in the colon, and inferior vena caval gas. Her condition improved after treatment with granulocyte colony-stimulating factor and broad-spectrum antibiotics. We report on this case along with a review of the literature.

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.

“Onnuri” is a new orchardgrass(Dactylis glomerata L.) variety developed by the National Institue of Animal Science (NIAS) in 2011. To develope the new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. Agronomic growth characteristics and forage production of “Onnuri” were examined at Cheonan from 2009 to 2011, and regional trials were conducted in Cheonan, Pyungchang Jinzu and Jeju from 2009 to 2011, respectively. “Onnuri” showed medium type growth habit in fall and spring and medium in length of flag leaf and long upper internode. Plant height of “Onnuri” was more than 10cm that of standard variety, “Amba” and heading date was 5 days earlyer than 16th May compared to Amba. Characteristics such as waterlogging, disease resistance of “Onnuri” were stronger or better than those of Amba, specially “Onnuri” showed 18% higher dry matter yield (14,775kg/ha) compared to Amaba(12,523kg/ha). Nutritive value was appeared to be similar in both varieties.

Italian Ryegrass is a high productivity and feed value, and an upright grass that behaves like a biennial or short-lived perennial. It grows vigorously in winter and early spring. Italian ryegrass and a related species, perennial ryegrass, Lolium perenne, are the two common weedy ryegrassses. Italian ryegrass and perennial ryegrass can hybridize, resulting in offspring that are difficult to identify as either species. Ryegrasses are cultivated for turf and forage. Sometimes Italian ryegrass is grown as cover crop. It has the potential to produce high yields and, with proper management, can be high quality with good animal performance. To develop of a high quality, productivity and early variety, 20 varieties examined growth and yield characteristics. The heading date of Green farm with high cold tolerance was on 28 April. Most of Introduced varieties except Grazer have mid and late heading date.

The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.