해충방제용 미생물 선발을 위해 2018년도에 공원·산림·유기농 근권 토양 87지점을 수집하여 갈색거저리를 이용한 insect-bait법 및 자연조건에서의 감염사충으로부터 곤충병원성 곰팡이를 132균주 분리하였다. 분리된 균주는 점박이응 애, 복숭아혹진딧물에 대한 살충활성 스크리닝을 통하여 각 해충별 살충활성을 보이는 56균주를 선발하였다. 선발된 균주들은 rDNA의 ITS영역을 이용하여 분류동정 하였다. 추가 적용범위 확대를 위해 무잎벌(종령 유충), 거세미나방류 (3~6령 유충)와 같은 지하부 이동성 해충의 방제 가능성을 탐색하기 위해 선발 곤층병원성 곰팡이의 포자 현탁액의 관주처리 방법으로 살충활성을 검정 중이다.

경기지역에 발생하는 돌발 및 남방계 해충의 발생실태 조사결과, 겨울철 경기지역 평균기온이 전년보다 3.7℃, 평년보다는 1.2℃ 낮게 경과함에 따라 포도 재배지 꽃매미 평균 월동난 부화율은 35.6%로 전년 대비 60% 감소하였음. 블루베리 재배지 갈색날개매미충의 평균 월동난 부화율은 26.9%로 전년 대비 50% 감소하였으며, 겨울철 피해가지 제거로 부화약충의 밀도는 매우 낮았음. 저온 적응성이 강한 미국선녀벌레의 경우 6월 중순 안성지역 평야 블루베리에서 약충이 가지당 최대 6.64마리 발견되었으나, 하절기 고온 등으로 성충의 밀도는 크게 감소되었음. 남방계 해충인 볼록총채벌레는 포도 재배지에서 5월 하순부터 발견되기 시작하여 7월 상순부터 급격히 증가하는 양상을 보인 가운데 지역별로는 남부지역(안성), 지대별로는 중산간지 > 산간지 > 평야지 순으로 높은 경향을 보였음.

One of the leading pests of rice, small brown planthopper (Laodelphax striatellus) can grow up to have either short or long wings, depending on conditions. However, under the same breeding conditions, the phenotypes of the long- and short-winged small brown planthopper observed to keep the first collected phenotype. To investigate the mechanism involved in wing dimorphism, metabolomic researches have been conducted. In this study, we observed several metabolites change, and the difference of metabolites could provide clues to the relationship between physiological changes in the small brown planthopper and ecological transport.

A harpacticoid species, Paramphiascella fulvofasciata Rosenfield and Coull, 1974, belonging to Miraciidae Dana, 1846 is newly reported from Korean waters. The species is mostly related with P. pacifica Vervoort, 1962, however, it is easily distinguished by the typical structure of male P2 in having 1 large spine and 2 setae on P2 enp-2. Korean materials represented some discrepancies from the original description of P. fulvofasciata in the detailed structures of mouth parts. The present study is a first record of the genus Paramphiascella Lang, 1944 from Korean waters.

The purpose of this study was to investigate the distribution of trig˗ ger points(TrPs) on athletes with various sporting background. To achieve the purpose, a study was carried out through a survey from 180 athletes involved in 6 selected sports at Yong-In University. Selected sports included Judo, Taekwondo, Kendo, Ssi-reum(Korean traditional wrestling), Boxing, and Golf. An interview type survey and physical examination were conducted with each thirty athletes from each of the selected sports groups. Technical statistic(SPSS 15.0) was used to analyze the distribution of TrPs on these athletes. The most common TrPs observed in muscles of Trapezius, Quadratus Lumborum, Quadriceps in Judo. In Taekwondo, it was on the trapez˗ ius and triceps surae. Kendo athletes had TrPs at sites of trapezius, brachioradialis and triceps surae. Ssirem athletes were found to have TrPs on trapezius, deltoid and quadrates lumborum. In boxers, TrPs appearing at trapezius and brachioradialis were observed. Finally, Golf players were seen to have TrPs at trapezius, quadrates lumbo˗ rum and brachioradialis. Hence, the analysis shows that there are significant differences of the distribution of TrPs according to the different sport items of the athlete.

Sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many economically important crops. The insect has developed resistance to chemical insecticides. Therefore, the development of microbial agent is necessary. Among the several entomopathogenic fungi, Lecanicillium lecanii Btab01 which has high insecticidal activity was carried out this experiments. To develop mass culture, we subcultured L. lecanii Btab01 on PDA, TSA, SDA+Y, RA and GSA media at 25℃ incubator to select the optimal solid culture medium. Hyphal growth was measured every 3 or 4 days. L. lecanii Btab01 grew fastest in RA, followed GSA, SDA+Y, PDA and TSA. L. lecanii Btab01 was cultured on PDB, TSB, SDB+Y, RB, GSB media at 25℃, 180rpm shaking incubator to select the optimal liquid medium. Spore germination was measured by spread plate method every 12 or 24 hours. Spore germination appeared 7.8×108 CFU/ml after 4 days in RB, followed GSB (5.5×108 CFU/ml), SDB+Y (2.7×108 CFU/ml), TSB (1.7×108 CFU/ml) and PDB (0.6×108 CFU/ml).

Low temperature germinability (LTG) is an important trait for breeding of varieties for use in direct-seeding rice production systems. Although rice (Oryza sativa L.) is generally sensitive to low temperatures, genetic variation for LTG exists and several quantitative trait loci (QTLs) have been reported. The objective of this study was to develop and employ high-efficiency molecular markers for evaluation of LTG in rice germplasm. A panel of japonica rice accessions (n=180) from temperate regions in Asia was evaluated for LTG and genotyped with markers from regions previously reported to harbor other LTG QTLs. ANOVA revealed that four markers on chromosome 2, 4, and 11 from previously reported QTLs showed highly significant value (p < 1.0e-04) and their R2 ranged 0.083 (qLTG11-1) to 0.190 (qLTG4b-1). An association analysis was conducted using SNP data generated by sequencing of the panel. Eight SNP markers were found to be significantly associated with LTG using general and mixed linear models. Three SNP-based CAPS and dCAPS markers from these results were developed and showed higher accuracy in predicting sensitive LTG germplasm. These new LTG markers will be useful for molecular evaluation of germplasm, particularly to identify sensitive or weak LTG accessions.

Neutropenic enterocolitis (NE), the most serious gastrointestinal complication, has been reported as a clinical syndrome that occurs in the setting of disease- or chemotherapy-induced neutropenia. Complications of NE include bowel necrosis with perforation, fistula, stenosis, massive bleeding, abscess formation, and pneumatosis intestinalis (PI). Most physicians recommend initial conservative management with bowel rest, intravenous fluids, total parenteral nutrition, broad-spectrum antibiotics, and normalization of neutrophil counts. Surgical intervention is recommended in the event of obstruction, perforation, persistent gastrointestinal bleeding despite correction of thrombocytopenia and coagulopathy, or clinical deterioration. We experienced a patient whose abdominal computed tomography scan showed pneumoretroperitoneum, intramural gas in the colon, and inferior vena caval gas. Her condition improved after treatment with granulocyte colony-stimulating factor and broad-spectrum antibiotics. We report on this case along with a review of the literature.