The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.

열대지역에 적응하는 온대 자포니카 벼 품종 개발을 위한 이삭형질 개선의 육종 방향을 제시하고자 한국과 필리핀 우기재배에서의 이삭형질 변이 및 특성에 대해서 조사하였다. 이삭형질의 변이는 2차 지경 착생립수가 차이가 가장 많이 나는 형질이었고 1차 지경 평균립수가 가장 적게 나는 형질이었다. 2차 지경 관련 형질이 1차 지경 관련 형질보다 수당립수와의 상관관계가 높았다. 지역간 차이가 많이 난 형질은 2차 지경 립수, 2차 지경수, 수당립수, 총지경수 순

벼멸구 저항성 Bph18 유전자를 가진 SR30071-3-7-23-6-1-1-1계통과 감수성인 '새누리'를 교배하여 육성한 약배양집단 192계통에 대해 벼멸구 저항성 유전자 Bph18이 벼의 수량구성요소에 미치는 영향을 검토하였다. 벼멸구 저항성 유전자 Bph18과 밀접히 연관된 7312.T4A 마커의 유전자형을 분석한 결과, 저항성 유전자형을 가진 계통은 24계통이었고 감수성 유전자형을 가진 계통은 168계통이었다. 벼멸구 저항성 유전자 Bph18은

‘다청’은 국립식량과학원 벼맥류부에서 2008년도에 육성한 중만생 고품질 복합내병충성 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 남서해안지, 호남 및 영남평야지 보통기 보비재배에서 평균 출수기가 8월 19일로 ‘남평벼’보다 2일 늦은 중만생종이다. 2. 간장은 87 cm로 ‘남평벼’보다 크며, 수당립수는 많고 등숙비율이 다소 낮은 중립종에 속한다. 3. 위조현상은 나타나지 않았으며 성숙기 하엽노화가 늦고 수발아는 ‘남평벼’보다 잘 되는 편

‘호농’은 국립식량과학원 벼맥류부에서 운봉31호의 개화시에 MNU를 수정란(미숙배)에 처리한 후대에서 선발하여 2009년도에 품종보호권을 출원한 품종이다. 1. 남서해안지, 호남 및 영남평야지 보통기 보비재배에서 평균 출수기가 8월 19일로 ‘남평벼’보다 2일 늦은 중만생종이다. 2. 간장은 79 cm로 ‘남평벼’와 비슷하며, 수당립수는 적고 등숙비율이 다소 높은 중립종이다. 3. 위조현상은 나타나지 않았으며 성숙기 하엽노화가 늦고 수발아는 남평벼보다

Fermented soybean food is one of most economical and health food due to its valuable nutritional and medicinal attributes and have been consumed for centuries as flavoring ingredient in Korea. On fermented soybean food such as doenjang, aroma compounds are important property because they determine taste and grade of fermentation. This study investigated variety of aroma compounds of doenjang made from different soybean genotypes. Aroma compounds in twelve different doenjang made from two cultivar (Daewon and Taekwang) and ten elite lines were extracted by steam distillation extraction (SDE) method and analyzed by gas chromatography/mass spectrometry (GC/MS). Aroma compound were detected over 80 kinds in eight samples (Daewon, Taekwang, MY177, MY187, MY189, MY192, MY204 and MY205) and under 70 kinds in four samples (yeonchun1, MY188, MY203 and MY206). Among the detected aroma components, 47 compounds were assigned as aromatic compounds (21), long chain fatty acid (13), short chain fatty acid (5) and others (8) by the computer library (Wiley 7n program). The major aroma compounds in twelve different doenjang were benzaldehyde, benzeneacetaldehyde, 2-methoxy-4-vinylphenol, pyrazine type compounds, cysteaminesulfonic acid, palmitic acid, oleic acid and linoleic acid. Each doenjang made by same condition (microbe, temperature, aging time and etc) had a difference in ratio and variety of aroma compounds due to different components having soybean genotype.

Methyl jasmonate(MeJA) treatment promotes the plant secondary, especially triterpene saponin which is main compounds in Platycodon grandiflorum. We were performed 5,760 expressed sequence tags from MeJA treated hairy root. Total number of ESTs are 2,536 (811 contigs and 1,725 singletons) among them 2,299 are pass the annotation. The most abundant transcript were encoding fatty acid desaturase (FAD) and lots of secondary metabolite transcript were observed. Four MVA pathway genes (HMGS, HMGR, MK, MDD) and one MEP pathway gene (IDI) are identified. The unigene were classified into three major functional categories by standard GO (Gene Ontology) - cellular component, molecular function and biological process. In this study we were cloned four genes from ESTs : HMGS (1401bp ORF), MK (1167bp ORF), MDD (1254bp ORF), IDI (909bp ORF)

"드래찬" 품종은 국립식량과학원 벼맥류부에서 2008년도에 육성한 중만생 양질 내병성 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 출수기는 보통기 보비재배에서 8월 17일로 한마음보다 2일 늦은 중만생종이다. 2. 쌀은 복백이 약간 있으나 아밀로스 함량이 낮고 밥맛이 좋은 편이다. 3. 도열병, 흰잎마름병 레이스K1-3 및 줄무늬잎마름병에는 저항성 반응을 보였으나 멸구 및 매미충류에는 약한 반응을 보였다. 4. 유묘 내냉성은 한마음과 비슷하

"보석"은 국립식량과학원 벼맥류부에서 2008년도에 육성한 조생 고품질 내냉성 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 보통기 보비재배에서 평균 출수기가 7월 29일, 남부중산간지에서는 7월 26일로 오대벼보다 3일정도 빠른 조생종이다. 2. 오대벼에 해 주당수수와 수당립수는 많고, 등숙비율은 다소 높으나 현미천립중은 오대벼보다 가벼운 편이다. 3. 위조현상은 없고 성숙기 하엽노화가 늦고 수발아는 오대벼 보다 잘되는 편이다. 유묘기 내냉

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

Perilla frutescens, which belonging to the Labiatae family, is widely cultivated oil crop and have been used traditional herbal medicine in East Asia such as Korea, China, and Japan. Especially, the leaves and the seeds of this species are important in Korean traditional cooking, as one of the popular garnish and food colorants. Numerous studies have revealed that the beneficial health effects of perilla are due to its several phytochemicals contents, such as rosmarinic acid, caffeic acid, luteolin, and apigenin. For this reason, increasing the content of phytochemicals in perilla hasbecome a major breeding objective. The genetic diversity of the rosmarinic acid, caffeic acid, luteolin, and apigenin content in perilla seed is poorly documented. We analyzed the rosmarinic acid, caffeic acid, luteolin, and apigenin content of 203 accessions of perilla germplasm by high performance liquid chromatography (HPLC). The rosmarinic acid and luteolin contents ofgermplasms were ranged from 15.7 μg/g to 2717.1 μg/g and from 1.6 μg/g to 582.4 μg/g respectively.