본 연구는 여성 독거노인의 심리적 고독감과 삶의 만족도 의 관계에서 사회적 지지의 매개효과를 검증하고자 하였다. 본 연구는 D시에 거주하는 여성 독거노인을 대상으로 2022 년 7월 1일부터 8월 31일까지 300명의 자료를 수집하였으며, 이 중 290명의 자료를 분석에 활용하였다. 자료분석은 빈도 분석, 기술통계, 상관관계 그리고 구조방정식 분석을 하였다. 분석결과, 첫째, 여성 독거노인의 고독감은 삶의 만족도에 유 의미한 부(-)의 관계의 영향을 확인하고, 사회적 지지와 삶의 만족도는 정(+)의 방향에서 유의미한 영향을 이루는 것을 확 인할 수 있다. 둘째, 여성 독거노인의 고독감과 삶의 만족도 의 관계에서 사회적 지지의 매개효과를 확인할 수 있었으며, 사회인구학적 특성인 경제적 상태의 영향력을 확인하였다. 이를 바탕으로 여성 독거노인의 삶의 만족도를 증진할 수 있 는 방안을 제시하였다는 점에서 의의가 있다.

We checked the presence of phospholipase A2(PLA)2 which could split the ester bond at the position 2 in the glycerol backbone of glycerophospholipids, in the cells of hyperthermophiles of Pyrococcus horikoshii and Sulfolobus acidocaldarius. The results obtained are as follows; (1). Pyrococcus horikoshii cells were grown in obligate anaerobic conditions at 95℃ and they needed sulfur as energy source instead of oxygen, while Sulfolobus acidocaldarius species grew well in the aerobic medium (pH 2.5) containing yeast and sucrose at 75℃. (2). Pyrococcus horikoshii cells produced phospholipase A2 in the cell culture media although this species did not show lipase activity at least in the pH range of 1.5 ~ 3.5. Sulfolobus acidocaldarius cells produced lipase hydrolyzing triacylglycerols such as triolein, but did not split any kind of phospholipids used as substates. (3). The compound of 1-decanoyl-2-(p-nitrophenylglutaryl) phosphatidylcholine was not suitable for a substrate in this experiment, though frequently used as a subtrate for checking presence of phospholipase A2, for its decomposi-tion in this experiment. The L-α-phosphatidylcholine-β-[N-7-nitrobenz-2-oxa-1, 3-diazol]aminohexanoyl-γ-hexadecanoyl labelled with a fluorescent material, did not show any migration of acyl chains in the molecule during the reaction with phospholipase A2 under a hot condition. (4). Phospholipase A2 in the cells of Pyrococcus horikoshii, showed the optimum activity at pH6.7~7.2 and 95~105℃, respectively, and was activated by addition of calcium chloride solution. Andthe phospholipase A2 specifically hydrolyzed glycero-phospholipids such as phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine and phosphatidyl inositol, but could not split phospholipid containing ether bonds in the molecule such as DL -α-phosphatidylcholine-β-palmitoyl-γ-O-hexadecyl, DL-α-phosphati- dylcholine-β- oleoyl-γ-O-hexadecyl, DL-phosphatidylcholine-dihexadecyl.

The fatty acid, all cis-δ5,11,14-C20:3, in the Gingko nuts oils, was isolated and, purified by urea-adduct method, silver ion silica gel chromatography and HPLC equipped with reversed phase μ-Bondapak C18 column. Its structural elucidation was conducted by IR and 1H-, 13C-NMR technique. The fatty acid composition of seed oils mainly consists of linoleic acid(37.73%), vaccenic acid(18.30%), oleic acid(15.18%), palmitic acid(3.37%), palmitoleic acid(3.37%) and δ5 NMDB fatty acids(8.50%) in which all cis-δ5,11,14-C20:2 predominates.