Deoxynivalenol (DON) and related trichothecene mycotoxins are extensively distributed in the cereal-based food and feed stuffs worldwide. Recent climate changes and global grain trade increased chance of exposure to more DON and related toxic metabolites in poorly managed production systems. Monitoring the biological and environmental exposures to the toxins are crucial in protecting human and animals from toxicities of the hazardous contaminants in food or feeds. Exposure biomarkers including urine DON itself are prone to shift to less harmful metabolites by intestinal microbiota and liver metabolic enzymes. De-epoxyfication of DON by gut microbes such as Eubacterium strain BBSH 797 and Eubacterium sp. DSM 11798 leads to more fecal secretion of DOM-1. By contrast, most of plant-derived DON-glucoside is also easily catabolized to free DON by gut microbes, which produces more burden to body. Phase 2 hepatic metabolism also contributes to the glucuronidation of DON, which can be useful urine biomarkers. However, chemical modification could be very typical depending on the anthropologic or genetic background, luminal bacteria, and hepatic metabolic enzyme susceptibility to the toxins in the diet. After toxin exposure, effect biomarkers are also important in estimating the linkage and mechanisms of foodborne diseases in human and animal population. Most prominent adverse effects are demonstrated in the DON-induced immunological and behavioral disorders. For instance, acutely elevated interleukin-8 from insulted gut exposed to dietaty DON is a dominant clinical biomarker in human and animals. Moreover, subchronic exposure to the toxins is associated with high levels of serum IgA, a biological mediator of IgA nephritis. In particular, anorexia monitoring using mouse models are recently developed to monitor the biological activities of DON-induced feed refusal. It is also mechanistically linked to alteration of serotoin and peptide YY, which are promising biomarkers of neurological disorders by the toxins. As animalalternative biomonitoring, huamn enterocyte-based assay has been developed and more realistic gut mimetic models would be useful in monitoring the effect biomarkers in resposne to toxic contaminants in the future investigations.

본 연구는 해구별 침적폐기물 추정량을 산정하여 해구별 정화관리 우선순위를 분석하기 위한 전제로서 침적폐기물 정화관리지표를 개발하는 것이 연구목적이다. 이러한 연구목적을 달성하기 위한 연구방법으로 전문가 델파이 조사기법을 활용하였다. 전문가 델파이 기법은 적 절한 예측방법을 찾을 수 없을 때, 전문가들의 직관을 동원하여 미래를 예측하는 방법으로써 미래변화 뿐만 아니라, 합의를 도출하여 문제를 추정하거나 구성원의 의견을 수집․수렴하는 연구방법이다. 본 연구에서는 27명의 전문가를 섭외하여 이들을 대상으로 총 3회기에 걸친 델파이 조사를 실시하였다. 전문가 델파이 조사결과 최종적으로 선택된 연근해 침적폐기물 정화관리지표는 수거 후 재침적율, 폐어구 유실구역, 해구 별 총 폐기물량, 과거 침적폐기물 수거량, 단위면적당 폐기물량 등의 5개 지표로 도출되었다. 본 연구는 패널의 수가 27명으로 한정되어 있는 문제점이 존재하는데, 본 연구를 선행연구로 하여 향후 패널 수를 더욱 확대하여 정밀한 조사가 이루어지기를 바란다.

HACCP methodology was applied in the post-harvest processing and storage of domestic medicinal produces. Particularly in terms of mold and mycotoxin contamination, candidate critical control points (CCP) in the conventional practice in Korean farms were selected and monitored by comparing with on the standard guided processing and storage. When each processing of Angelicae Gigantis Radix were assessed for their safety, the drying steps such as the sun drying or the thermal drying depending on each farm made differences in mold contamination. Moreover, the storage conditions before or after the processing were another critical determinant in the fungal contamination. In other words, storage under 4˚C rather than at room temperature was favorable for reducing mold growth in the harvested crops. Occurrence rate of Aflatoxin B1 (AFB1) in Angelicae Gigantis Radix were 12.8%, but amount of AFB1 in all the collected samples were below 10 ppb regulatory limit allowed in Korea. However, for a few samples of Angelicae Gigantis Radix, still relatively high levels of total amount of the major aflatoxins (aflatoxin B1 + B2 + G1 + G2) were observed around 0.18~49.94 ppb, which is not regulated presently in Korea. It thus can be suggested that post-harvest processing and storage of Korean medicinal crops need further investigation and monitoring to establish the Good Agricultural Practice (GAP), particularly to minimize microbial risk including mold and mycotoxin contamination under the changing climate. Additionally, it is also warranted for new enacting of regulatory limits for total aflatoxins in the medicinal crops.