This study was conducted to evaluate the hangover relieving effect of algae and plant complex extract. The complex extract showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging effect in a concentration-dependent manner and high antioxidant capacity (59.48%) when administered at 120 μg/mL. In addition, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities were increased according to the concentration of the complex extract. A locomotor activity test with an alcohol-complex extract mouse group showed improved motor activities over that of the alcohol-water group at 120 min post-administration. Blood was harvested from each mouse at 120 min post-administration then concentrations of alcohol and aldehyde were measured. The alcohol-extract treated group showed significantly lower (p<0.0001) alcohol (33.48 ± 0.59 μg/mL) and aldehyde (1.54 ± 0.13 μg/mL) concentrations than the alcohol-water treated group. These results suggest that the hangover relieving effects of complex extract results in increased motor activity of mice, which reduces the alcohol and aldehyde concentration in the blood.