본 연구는 충청남도농업기술원 화훼연구소에서 새롭게 육성된 가든멈 신품종 Chrysanthemum×morifolium ‘CandyBall’과 ‘Fancy Ball’의 고품질 생산을 위한 최적 적심 처리횟수와 식물생장조절제 daminozide 살포 최적 농도를 알아보고자 수행하였다. 적심 처리횟수를 정식 1주 후 2주간격으로 1, 2, 3회 하였을때 ‘Candy Ball’과 ‘FancyBall’ 두 품종 모두 적심 처리에 의하여 초장이 크게 줄어들지는 않았으나 적심횟수가 많을수록 개화수가 증가하는 경향을 보여 생산 품질을 향상할 수 있을 것으로판단되었다. Daminozide 처리 농도 실험에서는 0, 1000,2500, 5000mg•L−1의 농도로 3회 엽면 살포시 두 품종모두 daminozide 처리 농도가 증가함에 따라 초장과 화경장이 감소하였다. 두 품종 모두 daminozide 처리에 의한 꽃 크기 변화는 없었으나, ‘Fancy Ball’ 품종의 경우daminozide 처리 농도가 높아질수록 붉은 화색이 옅어짐을 보였다. 두 품종 모두 daminozide 처리 농도가 증가함에 따라 개화수가 증가하는 결과로 전체 화기 건물중및 지상부 건물중이 증가하여 가든멈 품질이 향상됨을 알수 있었다. 결과적으로 가든멈 신품종 ‘Candy Ball’과‘Fancy Ball’의 고품질 생산을 위하여 단독 적심 처리보다는 2500-5000mg•L−1 농도의 daminozide 처리가 초장을 줄이고 개화수를 높이는데 효과적인 것으로 나타났다.

Experimental Study was carried out for benzene desorption by purge gas or evacuation in an activated carbon bed. As purge gas flow rate increased, desorption rate increased due to the higher interstitial linear gas velocity. For various purge gas flow rates, desoption curves almost got together if they were plotted against dimensionless time. At a higher flow rate, mass transfer zone became narrower. Temperature drop in the bed was more fast and severe at higher flow rates and higher outer temperature. It was found out that desorption was almost completed when the temperature in the drop of the bed returned to the initial temperature before temperature drop. Desorption by vacuum purge was completed in shorter time than desorption by purge gas. Countercurrent purge was more effective than cocurrent purge.

The VOCs(Volatile Organic Compounds) is one of the major cause for the atmosphere pollution. Breakthrough behavior of benzene and toluene in adsorption bed packed with activated carbon was experimentally studied. Composition and temperature of the gas flowing in the bed was measured and breakthrough curves for each component was obtained. Breakthrough time of benzene was earlier than that of toluene due to relatively weak adsorptivity. The relationship between breakthrough time and flow rate was obtained. The shape of temperature change with time was dependent on the position in the bed. Temperature changed faster and sharper in the inlet than in the outlet. It was noted that breakthrough behavior could be affected by the heat transfer properties.

This study was carried out to investigate the effect of sucrose, boric acid, and calcium nitrate in optimum pH and relative humidity on pollen germination and pollen tube growth of boxthorn (Lycium chinense M.). Medium containing 100mg/L of H3BO3 showed maximum pollen germination and pollen tube elongation, 64.8% and 920μm. Additionally supplied calcium nitrate to the medium lowered pollen germination rate and pollen tube elongation. Here we report the optimum conditions for the pollen germination and pollen tube development was observed at 10% sucrose, 1.0% agar, pH 6.0, and 100% relative humidity.

Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.

본 연구는 random primer를 이용하여 PCR을 수행하기 위한 딸기 DNA증폭의 최적조건을 구명하여 조직배양된 딸기 배양묘와 모본과의 유전적인 동일성의 여부 및 품종을 판별할 수 있는 marker를 개발하기 위하여 수행하였다. 추출한 딸기 커(\ulcorner)잣 DNA를 proteinase-K나 RNase-H를 처리하였을때 깨끗하고 순수한 DNA band를 확인할 수 있었으며, 50ng의 template DNA, 10pmol의 primer, 37oC annealing 온도로 45 cycle로서 PCR을 행하는 것이 가장 효율적이었다. 상기 실험결과로서 PCR적정조건을 확립한 후, UBC primer를 대상으로 딸기 여봉 DNA에서 PCR를 수행하여 RAPD의 양상을 조사한 결과 총 90개의 primer 중에서 딸기 genomic DNA에서 PCR product를 형성한 것은 46개였으며, 총 형성된 band의 수는 158개로 나타났다. Band를 형성한 primer와 band를 형성하지 않은 primer간의 GC content를 비교하면 band를 형성한 primer의 경우 GC content는 평균 67.4%이었다. 그러나 band를 형성하지 못한 primer의 경우에는GC함량이 평균58%이었다.