Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits Aβ (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to 100 μM hydrogen peroxide (H2O2) induced neuronal apoptotic death. At 10-100μg/ml, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by H2O2 in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 mg/kg). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcumalongae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid βprotein (Aβ) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to10µM Aβ (25-35) for 36h induced neuronal apoptotic death. At 1-50㎍/㎖, HS0608 inhibited neuronal death, elevation of intra-cellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ (25-35) in primary cul-tures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol Aβ (25-35) wasinhibited by chronic treatment with HS0608 (25, 50 and 100㎎/㎏, p.o. for 7 days) as measured by a passive avoidance test. Fromthese results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against Aβ (25-35)-inducedneurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer’s disease.

송어장 배출수를 이용한 고추냉이 재배 가능성을 확인하기 위해 강원도 평창군 평창읍 상리 송어양식장에서 '95년 5월부터 '96년 5월까지 13개월동안 대왕 001와 달마종의 6개월묘를 공시하여 재배환경, 생육특성 및 수량성과 꽃대 제거에 따른 근경비대 정도 등을 시험한 결과는 다음과 같다. 1. 재배기간중 하우스내 연평균 기온은 14.3℃ 로 8월에 최고기온 27.5℃ 를 나타냈고 2월에 최저기온 -0.7℃ 를 나타냈으며 연평균 수온은 12.2℃ 로 8월에 최고수온 16.5℃ , 2월에 최저수온 9.0℃ 를 나타냈는데 기온과 수온이 고추냉이 생육에 가장 적합한 계절은 5월과 10월이었으며 이때 가장 왕성한 생육을 보였다. 2. 송어양식후 수중에는 Mg을 제외한 무기성분 함량이 증가한 반면 용존산소량은 감소되었는데 8월에는 용존산소량이 6.7ppm으로 급격히 저하되어 고추냉이 생육에 필요한 용존산소의 부족을 초래했다. 3. 대왕 001와 달마종의 개체당 주근경중은 각각 평균 78.7g, 37. 1g으로 대왕 001는 주근경의 비대가 양호해 상품률이 90.0%로 매우 높은 반면 달마종은 얼자의 발생이 심해 상품성 있는 40g 이상의 근경 비율이 37.5%로 매우 낮았으며 대왕 001와 달마종의 10a당 주경의 근경수량은 각각 608, 287kg였다. 4. 대왕 001는 개체당 11.1개의 꽃대가 발생하였으며 2회 꽃대 제거시 주경의 근경중이 21%증가되었다.