국내 유통 주류 95점에 대하여 에틸카바메이트 잔류실태 조사를 실시하였다. 탁주, 약주, 청주, 과실주를 비롯하여 소주, 브랜디 그리고 에틸카바메이트 잔류가 많이 보고되고 있는 리큐르 등을 대상으로 하였다. 액액분배를 통하여 정제하였으며, GC/MS/MS 분석법을 정립하였다. 카트리지(cartridge)나 농축 과정이 없기 때문에 식품공전에등재되어 있는 GC/MS 분석법보다 분석시간이나 소요비용 면에서 매우 효율적이었다. 정성한계는 1.3 ug/L이었으며, 정량한계는 4.0 ug/L이었다. 탁주, 약주, 청주에 대하여각각 0.63, 7.01, 14.11 ug/L의 평균 잔류량을 나타내 캐나다의 청주 허용기준 200 ug/L나 약주, 청주에서 논의되고있는 허용기준치 200 ug/L와 비교하여 보았을 때 안전한수준인 것으로 나타났다. 복분자주, 포도주에서는 각각 평균 1.66, 2.64 ug/L 검출되어 현재 캐나다, 체코에서 포도주허용기준치로 설정되어 있는 30 ug/L와 비교하여 보았을 때역시 안전한 것으로 나타났다. 과실주 중 매실주의 경우 평균 79.18 ug/L로 본 연구를 통하여 평가된 모든 주종 중 가장 높은 잔류량을 나타내었으나, 외국의 다른 유사 주종에대한 허용기준치 (예로서 캐나다의 경우 400 ug/L 과실 브랜디)와 비교하여 보았을 때 안전한 수준으로 평가할 수있었다. 증류식 소주, 일반증류주, 리큐르도 논의되고 있는기준치와 비교하여 보았을 때 안전한 것으로 조사되었다. 이러한 조사결과는 우리술의 에틸카바메이트 잔류실태가위험한 수준이 아니라는 근거 자료로 활용할 수 있을 것이며, 향후 이러한 유해물질의 지속적인 잔류조사 및 위해평가를 통하여 우리 술 및 전통발효식품의 품질 및 안전성을 확보해 나가야 할 것이다.

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 μg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 μg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 μg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 μg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 μg/kg for them, individually.

Nivalenol (NIV), deoxynivalenol (DON), T-2 toxin (T-2) and zearalenone (ZEN) are mycotoxins produced by some Fusarium species known to be very frequently contaminated in feed. The study for simultaneous analysis and contamination survey in animal feed carried out. All mycotoxins were analysed by using high performance liquid chromatography tandem mass with internal standard. The limits of detection (LOD) were 2.0 μg/kg,1.0 μg/kg, 1.0 μg/kg and 0.1 μg/kg for NIV, DON, T-2 and ZEN, respectively. Two hundred and thirty nine samples of feed were collected. The average concentration of DON was 212.3 μg/kg, 207.8 μg/kg and 812.1 μg/kg in chicken,pig and cattle feed, respectively. The average concentration of ZEN was 31.2 μg/kg, 35.6 μg/kg and 147.2 μg/kg for them, respectively. Especially, the levels of contamination for DON and ZEN were higher than those of NIV or T-2. And, the levels of contamination for four Fusarium mycotoxins in cattle feed appeared higher than those of pig and chicken feed. It was investigated that the high level of mycotoxin contamination in cattle feed was caused by corn gluten feed of ingredients for feed, mainly.

Aflatoxin (AF) and Ochratoxin A (OTA) are carcinogenic and possible carcinogenic mycotoxins respectively produced by Aspergillus spp or Penicillium spp. The study for contamination survey and proposal for reduction of mycotoxin in red pepper were carried out. 192 samples were collected at such various stages and markets as pre/post-harvest stages, internet shopping mall /super-market and small stakeholder mill/geographically indicated company. As only 2 samples were positive for aflatoxin, so contamination rate was 1.04%. In the meanwhile, contamination rate for ochratoxin A was 21.88% and a various amount of OTA was detected in 42 positive samples. 6 samples were found to be contaminated at higher level than 5 μgkg−1 for ochratoxin A, which was established recently as a maximum permissible limit in korea. There was no difference in degree of contamination with regard to cultivation type because any mycotoxin was not found at all in both organically and conventionally grown red pepper. But, there was statistically significant difference in the process of manufacturing. Finished products were OTA-contaminated at a level of 2.32 ± 6.54 μgkg−1(mean ± SD), even though OTA was not detected in deep frozen red peppers right after long term storage. And contamination for OTA was a level of 0.33 ± 0.91 μgkg−1(mean ± SD) in red paprika powder after uv sterilization, while the contamination for OTA was 2.78 ± 4.49 μgkg−1(mean ± SD) in non-uv sterilized powder. In addition, our investigation shows that higher OTA contamination occurred in some of famous brand products sold in super-market and domestic products than products collected through on-line shopping or from small stakeholder mills and imported products respectively, however, difference was not statistically significant.