The genus Desmodesmus (Chodat) S.S. An, T. Friedl & E. Hegewald is ubiquitous in freshwater ecosystems, such as rivers, ponds, and wetlands. The actual species diversity and distribution of the genus is unknown because of morphological plasticity affected by habitats. Currently, 38 Desmodesmus species have been reported in Korea most of which transferred from the genus Scenedesmus recently, however, no phylogenetic relationships have been studied yet. Despite the challenges in analyzing relationships among Desmodesmus species through the morphology, ecology, and original description, this study focused on examining species-level relationships using the FBCC culture strains isolated from Korea. A total of 299 sequences (66 of 18S rRNA, 47 of atpB, 67 of petA, 52 of rbcL, and 67 of tufA) were newly determined and used for phylogenetic analysis. Four plastid genes tend to have higher variation than 18S rRNA in the variable sites and P-distance. From the combined phylogeny, the Desmodesmus included six clades such as Clade-1: D. pseudoserratus and D. serratus, Clade-2: D. communis, D. dispar, D. maximus, D. pannonicus, unidentified Desmodesmus sp., Clade-3: D. bicaudatus and D. intermedius, Clade-4: D. microspina, D. multivariablis, D. pleiomorphus, D. subspicatus, Clade-5: D. abundans, D. kissii, and D. spinosus, and Clade-6: D. armatus, D. armatus var. longispina, D. opoliensis, unidentified Desmodesmus spp. The new sequence data from FBCC strains will be used to identify species and study the molecular ecology of scenedesmacean green algae in freshwater ecosystems. The phylogenetic information from this study will expand our understanding of Desmodesmus species diversity in Korea.
목적 : ‘치매관리법’에 따라 전국적으로 치매안심센터가 설립되고, 치매 관련 서비스를 제공할 수 있는 전문 인력의 요구도 증가되고 있다. 하지만 치매안심센터 조기검진팀의 구성원으로 작업치료사는 배제되어 있다. 따라서 치매안심센터 조기검진팀 구성원으로서 작업치료사의 필요성과 중요성을 알아보고자 하며, 이에 근거 자료를 제시하고자 한다.
연구방법 : 치매 평가영역에서 작업치료사의 역할에 대한 문헌을 중심으로 고찰하였으며, 작업치료사가 시행하는 평가도구 및 평가영역에 대하여 국내 및 국외 연구, 보건복지부 치매정책 관련 자료집과 치매센터 책자를 통하여 자료를 수집하였다. 치매안심센터 조기검진팀에서 시행하는 선별검사 및 신경인지검사와 작업치료사가 주로 사용하는 인지관련 평가도구의 항목들을 분석하였다.
결과 : 작업치료 수행영역에서 인지ᆞ지각영역의 평가 및 치료는 중요도나 수행도 측면에서 상위에 위치한다. 치매안심센터의 평가와 작업치료사들이 임상에서 사용하는 평가는 6가지 인지영역(기억력, 시공간 능력, 집중력, 실행능력, 계산능력, 언어능력)에서 중복되어 사용되고 있다.
결론 : 본 연구에서 작업치료사는 인지ᆞ지각 평가 영역에서 충분한 자질을 갖추고 있는 전문가임을 제시 하였기에 치매안심센터 조기검진팀의 구성원으로 포함되어야 하는 근거자료가 될 것이다. 본 연구는 앞으로 보건복지부에 치매정책관련 개정사항으로 의견 제출 시 유용하게 활용 될 것이다.
This study was conducted to evaluate the effect on quality characteristics of grass-fed cow’s milk (GM) and conventional feed-fed cow’s milk (CM) with various conditions of non-thermal high-pressure processing (HPP) sterilization process. The sterilized GM and CM stored at 5℃ and 10℃ for 14 days and their quality characteristics during storage was evaluated. The pH and titratable acidity changes of HPP sterilized milk were maintained acceptable level 6.7-6.8, 0.14-0.16%, respectively. HPP sterilization was efficient in the reduction of total bacterial population to decrease the level of 4-7 Log CFU/ml compare to those of unsterilized control milk, and coliform bacteria was not detected in the HPP sterilized milk. Non-thermal HPP sterilization processing was not affected milk fatty acid composition compare to those of low temperature long time process (LTLT) sterilized milk. GM has lower ratio of n-3:n-6 unsaturated fatty acid level than those of CM. In the sensory evaluation of GM and commercial milk products, overall consumer acceptance of HPP sterilized GM was higher than CM and LTLT commercial product. In conclusion, the effects of HPP was comparable with conventional LTLT sterilization process to control undesirable microorganism in the milk products with minumal nutritional and chemical changes.