The purpose of this study is to investigate the distribution of toxin genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in Gwangju. A total of 335 seafood, including 163 shellfish, 97 fish, and 36 mollusk, were tested in this study. As a result, V. parahaemolyticus was detected in 123 (36.7%) of 335 seafood. The tdh gene was not detected in all strains, while the trh gene was detected in 3 strains (2.4%). According to antimicrobial susceptibility test, 116 strains (94.3%) represent resistance to ampicillin, and 1 strain (0.8%) represents resistance to trimethoprim/sulfametoxazole. However, all strains were sensitive to 9 antimicrobial agents, including amikacin, chloramphenicol, tetracycline, and more. Therefore, the risk of V. parahaemolyticus isolated from seafood in Gwangju is considered low, but continuous monitoring of V. parahaemolyticus in seafood is required.

본 연구는 광주광역시에 유통·판매되고 있는 소고기를 대상으로 식중독 발생 비율이 높은 병원성대장균의 검출 여부와 분리된 균주의 항생제 내성 및 유전적 특성을 조사 하였다. 전체 335건의 소고기 중 82건에서 병원성대장균이 검출(24.4%)되었고 102개의 다양한 균주를 분리하였다. 분리균주의 병원성 유전자를 토대로 분류한 결과, EHEC가 66 균주로 가장 많았고 EHEC와 EPEC 등 2가지 유전자가 동시에 검출된 균주도 11균주가 있었다. 분리된 균주의 항생제 내성 시험결과, 30균주는 1가지 이상의 항생제에 대하여 내성을 보였다. 그 중 tetracycline에 내성을 보이는 균주가 27균주로 가장 많았고 그 외에 ampicillin, trimethoprim/ sulfamethoxazole, chloramphenicol 등의 순으로 많았다. 분리 된 균주의 혈청형 검사 결과, 혈청형은 O26, O91, O103, O104, ,O111, O113, O121, O128 및 O145로 확인되었다. 따라서 소고기의 조리 가공 시 교차오염이 발생하지 않도록 조리기구 등에 대한 위생적 관리와 충분한 조리 등의 식중독 예방을 위한 주의가 필요할 것으로 생각된다.

The purpose of this study was to investigate the prevalence, toxin gene profiles, and enterotoxin producing ability of Bacillus cereus isolated from environment-friendly vegetables and good agricultural practices (GAP) vegetables. A total of 49 vegetables including 40 environment-friendly vegetables and 9 GAP vegetables were tested. The Vitek 2 system was used to identify B. cereus and the PCR was used to detect 6 toxin genes, respectively. B. cereus was detected in 34 (69.3%) of 49 vegetables and the prevalence of B. cereus in GAP vegetables (44.4%) was lower than in the environment-friendly vegetables (75.0%). The detection rates of entFM, nheA, hblC, and cytK enterotoxin genes, respectively, among all isolates were 100%, 97.0%, 88.2%, and 73.5%, respectively. All of the isolates had at least one or more enterotoxin gene and 20 isolates (58.8%) had hemolysin BL enterotoxin producing ability. The risk of food poisoning from the environment-friendly vegetables and the GAP vegetables has been shown as constant. Thus, it is necessary to expand the supply of GAP vegetables showing lower B. cereus contamination than the environment-friendly vegetables. The characteristics of the environment-friendly vegetables and the GAP vegetables that must be consumed after cleaning should be disseminated to consumers regarding food poisoning prevention.