This experimental study was designed to investigate the effects of Chrysanthemi Flos extract (CFE) on the change of cerebral hemodynamics〔regional cerebral blood flow (rCBF) and mean arterial blood pressure(MABP)〕in normal and cerebral ischemic rats, and to determine the mechanism of action of CFE. This study was also designed to investigate whether CFE inhibit lactate dehydrogenase (LDH) activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. The results were as follows ; 1. CFE increased rCBF and increased MABP significantly in a dose-dependent manner. 2. The CFE-induced increase in rCBF was significnatly inhibited by pretreatment with indometh acin(IDN), an inhibitor of cyclooxygenase and methylene blue(MTB), an inhibitor of guanylate cyclase. 3. The CFE-induced increase in MABP was significnatly inhibited by pretreatment with IDN and MTB. 4. CFE 100㎍/㎖ significantly inhibited lactate dehydrogenase(LDH) activity in neuronal cells. 5. rCBF was significantly and stably increased by CFE (1㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. 6. In serum, by drawing from femoral arterial blood after middle cerebral arterial occlusion (MCAO) 1 hr and reperfusion 1 hr, sample group was significantly decreased IL-1β production compared to that of the control group. 7. In serum, by drawing from femoral arterial blood after MCAO 1 hr and reperfusion 1 hr, sample group was significantly decreased TNF-α production compared to that of the control group. 8. In serum, by drawing from femoral arterial blood after reperfusion 1 hr, sample group significantly increased TGF-β production compared to that of the control group. 9. In serum, by drawing from femoral arterial blood after reperfusion 1 hr, sample group increased IL-10 production compared to that of the control group. These results suggested that the mechanism of CFE was mediated by cyclooxygenase and guanylate cyclase, and CFE had inhibitive effect on the brain damage by inhibited LDH activity, IL-1β and TNF-α production, but accelerated TGF-β production and IL-10 production.thought that CFE should have an anti-ischemic effect on the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.

The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) of Cortex Acanthopanacis in rats, and to determine the effect of Cortex Acanthopanacis on phenylephrine (PE) induced contraction of isolated rat thoracic aorta. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure transducer (Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. The result of this experiment was as following; 1. Cortex Acanthopanacis did not effect the changes of rCBF and blood pressure significantly. 2. Contractions evoked by phenylephrine were decreased significantly by Cortex Acanthopanacis 3. L-NNA, ODQ, atropine and indomethacin significantly altered the relaxation of Cortex Acanthopanacis. 4. Propranolol did not change the relaxation of Cortex Acanthopanacis. These results indicate that Cortex Acanthopanacis did not change the rCBF and BP, but Cortex Acanthopanacis can relax PE induced contraction of isolated rat thoracic aorta and that this increasing contraction related to endothelium and various mechanism.

본 연구는 원예 및 조경용 식물로 최근 들어 관심을 받고 있는 옥잠화에 대한 개화습성, 수분 양식 및 결실특성에 대한 기초정보를 얻고자 수행되었다. 옥잠화는 주로 야간에 백색꽃이 개화하는 식물로 개화초기와 개화성기에는 대부분 풍매에 의해 수정되었고, 개화후기로 갈수록 착화 간격이 줄어들고 옥잠화 특유의 향기가 감소하여 풍매에 의한 착협율과 충매에 의한 수정율이 증가하는 것으로 조사되었다. 착협율과 결실율은 방임구 각각에서 33.3%와 24.3%로 나타났으며, 이에 비해 인공교배는 각각 65.0%와 40.9%로 나타나 인공교배가 착협율과 결실율에서 높게 나타났다. 개화기간에 있어서 하루 중 수정율은 개화성기인 19 : 00~20 : 00시에서 높았으며, 이때의 최적온도는 25~28℃ 이었다.