The purpose of this present study is to measure the changes of regional cerebral blood flow(rCBF) was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. Rhus verniciflua stokes increased the changes of rCBF in rats, significantly. The rCBF of Rhus verniciflua stokes was decreased by pretreated propranolol, l-NNA and methylene blue. But atropine indomethacin did not change the rCBF. Contractions evoked by PE 0.1μM were significantly inecreased by Rhus verniciflua stokes. Propranolol significantly altered the effect of Rhus verniciflua stokes, but l-NNA, ODQ and indomethacin did not change the relaxation of Rhus verniciflua stokes. These results suggest that Rhus verniciflua stokes can increase the rCBF and relax EP induced contraction of isolated rat aorta, and that is related to sympathetics and nitric oxide.

The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) of Cortex Acanthopanacis in rats, and to determine the effect of Cortex Acanthopanacis on phenylephrine (PE) induced contraction of isolated rat thoracic aorta. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure transducer (Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. The result of this experiment was as following; 1. Cortex Acanthopanacis did not effect the changes of rCBF and blood pressure significantly. 2. Contractions evoked by phenylephrine were decreased significantly by Cortex Acanthopanacis 3. L-NNA, ODQ, atropine and indomethacin significantly altered the relaxation of Cortex Acanthopanacis. 4. Propranolol did not change the relaxation of Cortex Acanthopanacis. These results indicate that Cortex Acanthopanacis did not change the rCBF and BP, but Cortex Acanthopanacis can relax PE induced contraction of isolated rat thoracic aorta and that this increasing contraction related to endothelium and various mechanism.