The purpose of this study was to analyze the effects of sewerage facilities through I/I analysis by rainfall by selecting areas where storm overflow diverging chamber is remained due to the non-maintenance drainage equipment when the sewerage system was reconstructed as a separate sewer system. Research has shown that wet weather flow(WWF) increased from 106.2% to 154.8% compared to dry weather flow(DWF) in intercepting sewers, and that the WWF increased from 122.4% to 257.6% in comparison to DWF in storm overflow diverging chamber. As a result, owing to storm overflow diverging chamber of partially separate sewer system with untreated tributary of sewage treatment plant, rainfall-derived infiltration/inflow(RDII) has been analyzed 2.7 times higher than the areas without storm overflow diverging chamber. Meanwhile, infiltration quantity of this study area was relatively higher than that of other study areas. Therefore, it is necessary to reduce infiltration quantity through sewer pipe maintenance nearby river. Drainage equipment maintenance should be performed not to operate storm overflow diverging chamber in order to handle the appropriate sewage treatment plant capacity for rainfall because it is also expected that RDII due to rain will occur after maintenance. In conclusion, it is necessary to recognize aRDII(allowance of rainfall-derived infiltration/inflow) and to be reflected it on sewage treatment plant capacity because aRDII can occur even after maintenance to the complete separate sewer system.

본 연구는 미세유체시스템 내부에 효소가 고정된 PEG 하이드로젤 마이크로 어레이를 이용한 알코올 감지 바이오센서 제작에 대한 방법을 소개한다. 모델 알코올인 에탄올은 알코올 옥시다아제(Alcoholoxidase,AOX)를 포함한 PEG 하이드로젤 마이크로 어레이에 의해 광학적으로 분석되며, 다중의 미세채널구조를 이용하여 동시에 여러 농도의 알코올을 측정할 수 있다. 에탄올과 알코올 옥시다아제와의 효소촉매작용을 통해 H2O2이 생성되며 이는 페록시다아제 (Peroxidase,POD)와 반응하여 무색의 Amplex Red reagent를 붉은 형광을 띄는 resorufin으로 바꿔준다. 결과적으로 형광측정을 통해 에탄올의 농도를 확인할 수 있다. 에탄올의 농도가 높을수록 H2O2의 생성이 많아지고 resorufin의 형광강도가 강해지는 메카니즘을 통해 손쉽게 측정이 가능하다. 여섯줄의 미세유체채널을 포함하는 미세유체시스템 내부에 각각 에탄올에 반응하는 알코올 옥시다아제가 고정된 PEG 하이드로젤 마이크로 어레이를 제조하고 체내의 에탄올 농도인 1.0∼10mM을 반응시켜 형광변화를 통한 동시 측정이 가능한 바이오센서를 제작하였다.

This study was carried out to design New Certification of Floral Arrangement based on the National Competency Standards to Using Competency Map. Currently the industrial base certification systems, not academic base, are emphasized. Because the industrial base certification system were derived by job analysis of industrial sites. The data were gathered by direct survey on industrial fields and internet homepages of HRD Korea and Q-net etc. and verified by brainstorming on the expert FGI and interview on the flower design industries. Certification events were 2 items such as ‘Unit Floral Arrangement L2’ and ‘Unit Floral Arrangement L4’, and critical duties and optional duties, and levels and required study time were derived. And Competency Map and lifecycle certification development paths were conducted, and the objectives and subjects, required time of education and training for expert and qualification of technical certification. In this study result, the certifications upto level 5 were suggested, if social and legal system would be served the certification level 8 or below could be developed and suggested. Finally news jobs, such as spatial floral arrangement and eco-friendly floral arrangement, are needed to be suggested and competency maps inked into unit flower design and certification events were developed.