큰느타리버섯의 저온성적응성 형질에 관련된 SCARmarker를 개발하기 위하여 저온성 계통의 8균주와 대조구 8균주의 genomic DNA를 30ug/ml의 농도로bulking한 것을 주형 DNA로 사용하고 operon 사의OPA(20개), OPB(20개), OPL(20개), OPP(20개),OPR(20개), OPS(20개) 등 총 120개 primer를 randomprimer(10mer)로 사용하여 RAPD를 수행하였으며 이중에서 OP-S3 primer를 사용한 PCR 산물들이 대조구와 가장 뚜렷한 차이를 나타내었다. OP-S3 primer를이용한 RAPD 결과, 약 480 bp 부근에서 저온성 계통에 특이적인 DNA band가 관찰되었으며 이 DNAband의 염기서열을 근거로 SCAR marker로 사용할specific primer인 OP-S3-1-F와 OP-S3-1-R를 디자인하였다. SCAR marker OP-S3-1 primer를 이용하여 PCR을 수행한 결과에서는 저온성 계통에서만 480 bp 부근에서 대조구와 구별되는 DNA band를 확인할 수 있었으며 random primer인 OP-S3 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA band를확인할 수 있었다.

How did the Korean religious culture which was consisted of three different religions- Shamanism, Buddhism, Confucianism- be combined and transformed? The author focused the mixture and transformation of the procedure of sacrificial rite and the arrangement of sacrificial food in each religion. In this thesis, the author studied first, the conception in sacrificial rite, second, the procedure of sacrificial rite, third, the items of sacrificial rite food according to each period. In consequence of the research each religion had lost its uniqueness and became mixed to each other and settled down in Korean culture.

The sacrificial rite has its origin in the old China's primitive folkways faith based upon animism (B.C. 25c). From the animistic faith, Confucianism made its appearance in B.C. 551. Inevitably, the procedure of Confucian sacrificial rite was developed on the basis of the preceding primitive faith. In Korean culture, the god of Chinese Confucianism introduced to Korea in A.D. 108 was mixed properly with that of Buddhism imported in A.D. 372. Traditionally, Korean primitive religion (from B.C. 10c to B.C. 2c) was the sacrificial rite practiced by 'shaman.' The 'shaman' who was able to utilize ecstasy for the good of community was gods itself, and naturally the main form of the sacrificial rite was an exorcism with a sacrificial offering (food). After Korean primitive religion had been grafted to Buddhism and Confucianism, the character of Korean culture had to become compound. The most essential conception in sacrificial rite is a discrimination of a ghost, one is the evil spirit and the other is the good spirit. According to this conception, the good spirit is a spirit which ascended to heaven, in contrast, the evil spirit is a one which did not ascend to heaven and dispersed into this world. The sacrificial rite is a method to help the evil spirit ascend to heaven or to prevent harms from it. The mode of sacrificial rite especially the dead ancestor worship was transmitted from generation to generation as a purpose of the wealth and honors of descendants. Descendants believed that the evil spirit would not harm them only after receiving sufficient food and the right sacrifice. As a result, the sacrificial rite food was the sign of filial piety and a compensation for the evil spirit. How did the Korean religious culture which was consisted of three different religions-Shamanism, Buddhism. Confucianism-be combined and transformed? The author focused the mixture and transformation of the procedure of sacrificial rite and the arrangement of sacrificial food in each religion. In this thesis, the author studied first, the conception in sacrificial rite, second, the items of sacrificial rite food according to each period. In consequence of the research, each religion had lost its uniqueness and became mixed to each other and settle down in Korean culture.