본 연구에서는 떡쑥 추출물의 항산화 활성 및 활성 성분을 확인하였다. 모든 실험은 떡쑥 추출물의 70%에탄올 추출물, 에틸아세테이트 분획, 아글리콘 분획을 이용하여 진행하였다. DPPH (1,1-diphenyl-2-picrylhydrazyl)법을 이용한 free radical 소거활성(FSC50)은 에틸아세테이트 분획(6.15 μg/mL)이 비교물질인(+)-α-tocopherol (8.89 μg/mL)보다 높게 나타났다. 루미놀 발광법을 이용한 Fe3+-EDTA/H2O2계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 떡쑥 추출물의 총 항산화능(OSC50)은 70% 에탄올추출물의 경우 1.60 μg/mL, 에틸아세테이트 분획의 경우 0.075 μg/mL, 아글리콘 분획의 경우 2.28 μg/mL로비교물질인 L-ascorbic acid (6.88 μg/mL)보다 큰 활성을 나타내었다. 사람 적혈구의 1O2로 유도된 세포손상에 대한 보호효과 실험에서 70% 에탄올 추출물과 아글리콘 분획의 τ50은 10 μg/mL에서 각각 52.0, 60.6 min으로 지용성 항산화제로 알려진 (+)-α-tocopherol (38.0 min)보다 더 큰 세포 보호 효과를 나타내었다. TLC와 HPLC를 이용한 아글리콘 분획의 성분분석 결과 luteolin, apigenin 등이 존재함을 확인하였다. 이상의 결과들로부터 떡쑥 추출물은 라디칼을 포함한 활성산소종을 소거하는 항산화제로서 응용될 수 있음을 시사한다.

본 연구에서는 붉은장목수수(Sorglum Bicolor M.) MeOH 추출물과 분획물들의 다양한 생리활성을 비교하기 위하여 항산화(DPPH assay, 환원력, 총 페놀 및 플라보노이드 함량), 항당뇨(α-Glucosidase 저해활성 및 α-Amlyase 저해활성), 항암(MTT assay) 활성을 관찰 하였다. 항산화활성의 경우 EtOAc fraction이 높은 총 페놀과 플라보노이드 함량을 나타내었으며, DPPH assay와 reducing power 결과 역시 EtOAc fraction과 MeOH fraction이 positive control로 사용한 α-tocopherol보다 우수한 활성을 나타냈다. α-Glucosidase, α-amlyase 저해능 평가 결과 D.W. fraction이 가장 높은 활성을 보였으며 n-BuOH fraction과 MeOH extract도 활성을 나타내었다. 암세포인 AGS, HT29, HCT116세포주에 대한 세포독성 연구인 MTT assay에서는 EtOAc, n-BuOH, D.W. fraction을 처리한 처리구에서 독성을 보였으며 이중 n-BuOH fraction이 모든 세포주에서 농도 의존적으로 세포독성을 가지는 것을 확인할 수 있었다. 연구결과 붉은장목수수의 추출물 EtOAc fraction의 항산화활성, D.W. fraction의 항당뇨활성, n-BuOH fraction의 암세포에 대한 독성과 관련된 화합물의 분리 및 구조규명에 관한 연구가 필요 할 것으로 사료되어지며, normal cell에 대한 MTT assay를 진행하여 항암활성에 관한 연구를 진행하여 붉은장목수수 추출물을 이용한 다양한 건강기능식품과 의약품 개발을 통해 건강 증진과 질병으로부터 보호 받을 수 있을 것으로 기대한다.

This study investigated the changes of antioxidant activity and α-glucosidase inhibitory effect of Jerusalem artichoke (Helianthus tuberosus) 100% methanol extracts by various heat treatment. The contents of total phenolic and flavonoid compounds in methanol extract tended to increased gradually with the rise of temperature to 180℃. The maximum yield of gallic acid (51.52 ± 2.17mg/g extract weight) and quercetin (13.39 ± 0.03mg/g extract weight) were obtained with extraction temperature of 180℃ for 120min. In addition, the improving extraction efficiency resulted in the increased biological activities, such as electronic donation ability (EDA, 90.36± 0.57%), reducing power (Abs 1.14) and α-glucosidase inhibitory effect (92.14 ± 1.14%). Overall, the results of this study indicate that the optimum conditions for the extraction process were an extraction temperature at 180℃ for 120 min, and will provide the basis for future research on the improving extraction yield of phenolic and flavonoid compounds.

In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with RC50 value of 10.5±1.4μg/ml, reducing power value 1.04 Abs (concentration of 1 mg/ml), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/ml). M80 extract showed the high content of total phenolic (319.7 mg GAE/ml extract) and total flavonoid (111.6 mg QE/ml extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging (R2=0.911 and 0.912, each extract) and reducing power (R2=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity (R2=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.

Eli이 wrote the unfinished drama, Sweeney Agonistes: Fragments 01 the Aristophanic Me/odrama after The Waste Land and just before bis Christian conversion. He himself put the subtitle of ‘melodrama’ in it in 1932 and later published tbis poem under the group of “Unfinished Poems," which proves the writer’s intricate intention and concerns for this work. In spite of bis thoughtful cODsideratioDs, this work has not much appealed to the public and the scholars as might be expected. This article is to discover the significance of this work as a preparatory step for Eliot’s later works. The survey of tbe experimental strategies of Sweeney Agonistes: Fragments 01 the Aristophanic Me/odrama has revealed an important fact that Eliot wrote this work as an experimenlal ground for Ihe effeclive delivering of his theme to the public. For tbis purpose be has attempted some stylistic aids from both genres, poetη and drama, in this work. Firsl of all, Eliot utilizes the epigrapbs by introducing two representative perspectives on sin and redemption, the heathen Hellenic and the Christian, and implies the themes of his later works would focus on the latter one, that is, the spirilual exploration of Chrislianity. AIso be adopts one of the dramatic devices, the doubleness, whicb is to proceed witb two patterns of life in one work, the superficial daily one and the deep spiritual one, at the same time. Practically, Eliot shows the life of tbe 1920’s American low class in the first fragment of “A Prologue" while the next fragment of “An Agon" presents the under-pattern of the spiritual awakening tbrough tbe main character, Sweeney, with the invilalion of Doris to the he cannibal island to convert her. To use the advantages of the poetic form, EJi이 adopts musicaJ methods such as the jazz accompaniment and the chorus, which not onJy make up the compressed content of the pJay but derive the unconscious, immediate emotionaJ e따cts from the public even in the ignorance of the spirituaJ meaning of death and Iife. EJiot is aJso foJJowing the ruJes of the meJodrama such as the coincidence and the deJaying of the conclusion. In the work, Doris is obsessed by the idea of coincidence about her affairs and this work ends with Sweeney’s mlsslon as a missionary unfulfilled, which should be continually pursued in Eliot's later works.

스테로이드 호르몬의 합성은 콜레스테롤로부터 시작되고 Steroidogenic acute regulatory protein(StAR)은 스테로이드의 합성과정에서 콜레스테롤을 미토콘드리아의 안으로 신속하게 운반하는 역할을 한다. 스테로이드 호르몬은 난소, 부신, 고환에서 합성되며 본 연구에서는 흰쥐 난소와 부신에서 StAr mRNA의 발현 양상을 in situ hybridization 기법을 이용하여 조사하였다. 난소의 경우 StAR mRNa는 프로게스테론