The Agriculture, Forestry and Fisheries Research Council within the Ministry of Agriculture, Forestry and Fisheries looks over the Agriculture, Forestry, and Fisheries Research and Development Project. Every five years, it revises the Agriculture, Forestry and Fisheries Research Basic Plan. Based on the information above, the following suggestions are made to implement domestic research projects. First, the strategic planning and promotion of research and development projects are needed to achieve the goals of the country and agricultural policy. Second, it is necessary to break up and diversify research assignments so that the project implementation reflects the trend of the time. Third, there is a need to develop a research partnership that strengthens cooperation among industry, academia, government, and research institutes to utilize technology seeds from fields other than agriculture. Fourth, it is necessary to improve field demonstration research projects where central and local research institutes, universities, private companies, agricultural cooperation, and farmers can participate. Fifth, follow-ups should be conducted at the end of research to advance research implementation systems. Finally, there is a need to build a new dissemination system which includes research results and policy.

The Japanese government, on the basis of the Food, Agriculture and Rural Areas Basic Act, announced the 4th Basic Plan for Food, Agriculture and Rural areas, at the same time the third Basic Plan for Agriculture, Forestry and Fisheries Research in March 2015. The Basic Plan is configured as improvement of food self-sufficiency rate, a stable food supply, agricultural maintenance and development, rural promotion. The new target for the calorie-based food self-sufficiency ratio has been lowered from the previous 50 percent by 2020 to 45 percent by 2025. The production target for table rice has been lowered, but production targets for rice for flour and rice for feed have been raised significantly - five times and 10 times the more than current production by 2025, respectively. The 4th Basic Plan included the important measures of 24 and the details practice challenges of 50 should be intensively promoted for the next five years. The new Research Basic Plan set 21 key targets research and development to solve problems promptly faced by producers with the aim of increasing income in agriculture and rural communities and 11 priority targets research and development to be steadily promoted under medium- to long-term strategy. In addition, it announced the necessary measures of 10 at three areas in order to promote more efficiency and effective in the Agriculture, Forestry and Fisheries Research. In Korea, it refers to the announcement contents of Japan where there is a need to formulate a development plan for Agriculture, Rural Areas and Food Industry Development Plan that meets the Korean situation.

본 연구에서는 볶음 처리 후 여주의 총 phenolics, 총 flavonoids, 갈변물질(MRPs, maillard reaction products), 항산화 활성 및 α-glucosidase 저해활성을 비교하였다. 또한 alkaloids 유도체와 쓴맛 저감 효과를 평가하였다. 볶음 온도 및 시간이 증가함에 따라 볶음 처리된 여주는 총 phenolics, 총 flavonoids, MRPs, 항산화 활성 및 α-glucosidase 저해활성 역시 증가하였으나, alkaloid계 화합물 및 쓴맛은 감소하였다. 특히 200℃에서 15분 동안 볶은 여주는 총 phenolics, 총 flavonoids, MRPs, 항산화 활성 및 α-glucosidase 저해활성이 가장 높았고 alkaloid계 화합물 및 쓴맛은 가장 낮게 나타났다. 이 결과로부터 볶음 여주는 쓴맛이 저감되고 이들 추출물은 식품에서도 잠재적인 천연 항산화제로 사용할 수 있을 것으로 판단되었다.

단핵균주 간 교잡을 통해 육성된 고품질ㆍ다수확 느타리버섯“청산”의 주요특성은 다음과 같다. 가. 균사생장적온은 27∼28℃이고 버섯발생 및 생육온도는 14∼17℃이다. 나. 갓색은 진회색이고 얕은깔대기형이며 발생형태는 다발형이다. 다. 대색은 백색이고 형태는 굵고 긴형이다. 라. 버섯의 균일성에 있어서 갓색과 갓형태의 이형개체 발생은 없었고, 배양기간에서도 지역간 균일한 결과를 나타내었다. 마. 균상재배 시 초발이소요일수는 20일 내외, 자실체 생육일수는 4일이며, 수량은 농가실증시험에 평균 38 kg/3.3㎡을 나타냈다. 바. 청산느타리버섯은 균사활력이 강하며 적정생육온도에서 진회색을 띄나 적정온도이상의 재배환경에서는 갓색이 회색으로 바뀌어 품질이 저하되므로 생육온도 관리에 주의하여야 한다. 사. 발이량이 많으므로 재배 시 환기조절에 주의하여야 하며 갓이 약해 부서지기 쉬워 수확 및 포장 시 특히 주의를 요한다.

Bacillus thuringiensis strains were isolated from the domestic soil and a strain was selected that had a new host range and high toxicity against agriculture insect pest. The 142 samples of soil were sampled from the mountains, paddy fields and patches, in Daejon, Chungnam, Chungbuk and Jeonbuk and used for the investigation. Sixteen B. t strains were isolated from 12 samples among collected samples. There were 11 strains that showed toxical activity on Plutella xylostella (Lepidoptera: Yponomeutidae), 7 strains on Spodoptera litura (Lepidoptera: Noctuidae), 5 strains on Arcte coerulea (Lepidoptera: Noctuidae), 5 strains on Culex pipiens pallens (Diptera: Culicidae) among the 16 isolated B. t strains. But there were not any strains that showed activity against Hyphanria cunea (Lepidoptera: Arctiidae) and Sitophilus oryzae (Coleoptera: Rhynchophoridae). And also some of B. thuringiensis strains showed insecticidal activity with 2, 3 or 4 kinds of insects. But there were also 3 strains that did not show any activities to the 6 insects which were used in the experiment. When examined with a phase-contrast microscope, the insecticidal crystal protein produced from 16 selected strains had 13 bipyramidal and 3 spherical shapes. The insecticidal bioactivity of the S. litura showed 100% mortality when there were 1.3×10⁷ (cfu/㎖) of CAB109 isolates.

포항지역 주민 500명을 대상으로 꽁치과메기의 기호도 조사를 한 결과를 요약하면 다음과 같다. 과메기 용어 인지 여부에 대해서는 대부분 응답자가 알고 있었으며, 과메기 시식 여부는 92.5%가 먹어 보았으며. 먹어 보지 못한 7.5%에 대해 그 이유를 알아본 결과 먹고 싶지 않았다가 61.3%를 차지하였다. 연령별 시식 여부 차이 분석에서 나이가 많을수록 대부분 과메기를 먹어 본 것으로 나타났다(p<0.05). 과메기를 먹는 때는 술안주로가 80.9%로 가장 많았다. 남(86.5%). 여(56.8%) 모두 술안주로 먹는 경우가 가장 많았다. 간식으로 먹는 경우도 남자(6.6%)보다 여자(16.3%)가 많게 나타났다(p<0.05). 또한 연령이 많을수록 간식으로 먹는 경우가 많았다. 이러한 결과를 놓고 볼 때 사철과메기띄 개발 또는 포항 꽁치과메기가 향토식품으로 자리잡기 위해서는 술안주로서의 과메기가 아니라 조리방법이나 스낵 등의 가공방법 개발 등을 통하여 반찬이나 간식으로 먹을 수 있어야 진정한 먹거리로서 자리잡을 수 있을 것이다. 과메기를 처음 먹은 계기는 주위의 권유로 먹은 경우가 57.9%로 가장 많았다. 연령별로 살펴보면 젊을수록 과메기를 주위의 권유로 먹은 경우가 많으며, 나이가 많을수록 먹음직스러워서 과메기를 처음 먹은 계기가 된 것으로 나타났다(p<0.05). 과메기 선호도는 92.5%가 과메기에 대해 호의적인 응답을 하였다. 남자가 여자보다 과메기를 더 선호하는 것으로 나타났다(p<0.05). 연령별로는 31∼40세 연령층이 과메기를 가장 선호하는 계층으로 나타났다. 과메기를 먹을 때 곁들여 먹는 것으로 과메기, 생미역, 초고추장으로 먹는 경우가 가장 많았다. 포항 지역 주민들은 과메기를 먹을 때 대부분 생미떡과 곁들떠 먹지만 초고추장 없이 먹는 경우도 적지 않았다. 대부분3∼10마리 정도 1회 먹는 것으로 나타났으며, 20마리 이상 많이 먹는 주민도 남녀 거의 유사하게 나타났다. 따라서 여성층의 과메기 애호가도 많다는 것(5.6%)을 알 수 있다(p<0.05).

This study was designed to improve the sensory characteristics and mitigate the bitter taste of Platycodon gradiflorum. It was aimed at investigating the qualitative properties of fermented P. gradiflorum after repeated steaming and drying treatments. P. gradiflorum was heated for 2 hr at 95℃, being the first and third treatments compared afterwards. Lactobacillus plantarum, at a concentration of 10%, was used as starter culture. As a result, the third steaming process and the addition of starter improved the physical and chemical properties of P. gradiflorum i.e., crude saponin and total polyphenol contents increased significantly. Moreover, P. gradiflorum steamed three times and fermented by L. plantarum showed the higher overall preference score. Our results indicated that the three-time steaming and drying was an effective manufacturing process for the production of high-quality fermented P. gradiflorum. Lactic acid-fermented P. gradiflorum also could have a potential use as a valuable resource for the development of functional products.

To investigate the technology trends in organic farming using journal article analysis, total 2,378 published articles in organic farming between 1974 and 2014 were analyzed. The number of the articles published in organic farming has been steadily increased since 2006. As for category, articles in the field of producing the safe organic products and management (A) were published relatively dominantly, especially division of nutrient and soil management (AA). In the foreign countries, lots of articles in environmental assessment and safety field (B) were published than Korea. the main institutes published the articles in organic farming were Swedish University, Sweden and US department of Agriculture, USA. In case of Korea, the articles in the field of environmental assessment and safety (B) were relatively merely published, especially division of environment in organic farming (BA). With this study, more research based on the environment and safety fields in organic farming should be focused on.

To investigate the technology trends in organic farming using patent analysis, total 1,026 patents in organic farming in Australia, EU, Japan, Korea and USA between 1974 and 2014 were analyzed. The number of the patents applied in organic farming was largest in Korea, followed by Japan and the USA. Since 2000, the patent application in Korea and USA increased drastically, while in Japan the patents in organic farming were applied steadily in the late 1980s. The applied patents in organic farming were mainly dominated in the field of organic production technology (AA) especially in Korea, while those in environmental assessment were merely applied. Most patent appliers were biased to private applier (80%) than national institutes including university. With this study, it is highly recommended that national interests and R&D on the organic farming fields are needed to meet the food safety.

본 연구에서는 한국 및 중국산 감초(Glycyrrhiza uralensis), 그리고 우즈베키스탄산 감초(Glycyrrhizaglabra)를 대상으로 추출용매, 추출온도, 추출시간 등 추출조건별 추출물을 제조하고 이들 추출물들의 추출 수율과 항산화 활성을 비교하여 최적의 추출조건을 선정하였다. 항산화 활성 중 자유 라디칼(1,1-phenyl-2-picrylhydrazyl,DPPH) 소거활성은 85% 에탄올로 60 ℃에서 6 h 동안 추출한 조건에서 한국 감초로부터 얻은추출물이 가장 높은 활성(46.05%)을 나타내었다. 루미놀 발광법을 이용한 추출물들의 총 항산화능 측정 실험과피부 광노화에 중요한 1O2으로 유도된 세포막 손상에 대한 추출물들의 세포 보호 효과를 측정한 실험 모두에서도 위의 조건에서 가장 높은 항산화 활성을 나타내었다. 특히, 한국 감초는 τ50이 116.4 min으로 비교 물질인(+)-α-tocopherol (28.5 min)보다 약 4 배나 높은 세포 보호 효과를 나타내었으며, 추출 수율은 18.75%로우즈베키스탄 및 중국 감초보다 각각 1.2 배 및 2.5 배의 추출 수율을 나타내었다. 따라서, 본 연구 결과는 항산화 소재로 화장품에 응용하기 위하여 감초로부터 추출물을 얻기 위한 최적의 조건은 85% 에탄올로 60 ℃에서6시간 동안 추출하는 것임을 보여주었다.

본 연구에서는 붉은장목수수(Sorglum Bicolor M.) MeOH 추출물과 분획물들의 다양한 생리활성을 비교하기 위하여 항산화(DPPH assay, 환원력, 총 페놀 및 플라보노이드 함량), 항당뇨(α-Glucosidase 저해활성 및 α-Amlyase 저해활성), 항암(MTT assay) 활성을 관찰 하였다. 항산화활성의 경우 EtOAc fraction이 높은 총 페놀과 플라보노이드 함량을 나타내었으며, DPPH assay와 reducing power 결과 역시 EtOAc fraction과 MeOH fraction이 positive control로 사용한 α-tocopherol보다 우수한 활성을 나타냈다. α-Glucosidase, α-amlyase 저해능 평가 결과 D.W. fraction이 가장 높은 활성을 보였으며 n-BuOH fraction과 MeOH extract도 활성을 나타내었다. 암세포인 AGS, HT29, HCT116세포주에 대한 세포독성 연구인 MTT assay에서는 EtOAc, n-BuOH, D.W. fraction을 처리한 처리구에서 독성을 보였으며 이중 n-BuOH fraction이 모든 세포주에서 농도 의존적으로 세포독성을 가지는 것을 확인할 수 있었다. 연구결과 붉은장목수수의 추출물 EtOAc fraction의 항산화활성, D.W. fraction의 항당뇨활성, n-BuOH fraction의 암세포에 대한 독성과 관련된 화합물의 분리 및 구조규명에 관한 연구가 필요 할 것으로 사료되어지며, normal cell에 대한 MTT assay를 진행하여 항암활성에 관한 연구를 진행하여 붉은장목수수 추출물을 이용한 다양한 건강기능식품과 의약품 개발을 통해 건강 증진과 질병으로부터 보호 받을 수 있을 것으로 기대한다.

In general, stepwise hot steaming process is known to be effective in improving its biological activities; however, not much employed in processing Codonopsis lanceolata due to its hardness. In this study, C. lanceolata was first pretreated with warm water at 50℃ and 60℃ for two hours, then steamed for 3 hours. Antioxidant activities of 70% ethanol extracts were compared with the extract from the water solvent: 41.58% vs 8.98% of DPPH radical scavenging activity in adding 1.25mg/ml of steamed extract and water extract, respectively. Reducing power of steamed and fresh C. lanceolata were also measured as 1.39 and 0.71. Total poly phenolic of the steamed extract was estimated as 12.11mg/g, compared to 3.98mg/g fresh C. lanceolata. Total flavonoid contents were also obtained as 11.48mg/g, compared to 7.11mg/g of fresh C. lanceolata. In comparing phenolic acids profiles in the extract, in general higher amounts of gallic acid, trans-ferulic acid, vanillic acid were obtained possibly by easy release of active components during thermal processing, which results in better antioxidant activities than that of water extract. This findings can also be supported by result that the ethanol extract showed better activities than the water extract.

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging (IC50=0.50μg/mL). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p 〈 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

This study was carried out to investigate the effect of Lythrum salicaria L. ethanol extract on anti-obesity effects in rat fed a high fat diet for 8 weeks to induce obese rat model. Male SD rats were divided into normal group, control (high fat diet) group, positive control (Garcinia Cambogia extracts) group, high fat group supplemented with ethanol extracts of Lythrum salicaria L. (EELS). The body weight gain and control (high fat diet) were increased by a high fat diet, but decreased in the EELS. At the end of the experiment, the body weight in high fat diet groups was higher than that of normal diet group, while the body weights of EELS and positive control group were significantly reduced by 16.62%, as compared with that of high fat diet group (p < 0.05). The levels of serum triglyceride, total cholesterol in EELS group were significantly decreased as compared with high fat diet group (p < 0.05). The liver and mesenteric adipose tissue weights of control (high fat diet) increase than that for normal group, whereas EELS and positive control group were significantly decreased (p < 0.05). Levels of triglyceride in liver were significantly lower in EELS group than those in high fat diet group (p < 0.05). These results indicate that Lythrum salicaria L. extract may improve lipid metabolism and reduce fat accumulation and body weight.

In this study, the bioactivities of ethanol (EELS) and water extract (WELS) from the leaf of Lythrum salicaria L. were investigated. In the anti-cancer activity, the growths of both human prostate cancer (DU145) and human colonic carcinoma cell (HT29) were inhibited up 60% by adding 10 mg/ml of EELS. Anti-inflammatory activity of EELS and WELS have been evaluated on lipopolysaccharide (LPS) induced release of nitric oxide (NO) by the macrophage RAW 264.7 cells. EELS and WELS inhibited inflammatory by 57.3 and 46.9% in 10 mg/ml, respectively. In the anti-oxidative activity, IC50 of DPPH radical scavenging activity was respectively 60.71 and 92.90 μg/ml by EELS and WELS. In the anti-diabetic activity, IC50 of α-amylase inhibitory activity of EELS and WELS were respectively 5,250 and 5,020 μg/ml. IC50 of α-glucosidase inhibitory activity was 7.96 and 68.41 μg/ml by EELS and WELS. In the anti-obesity, IC50 of lipase inhibitory activity was 880 and 9,840 μg/ml by EELS and WELS. Finally, EELS and WELS exhibited anti-oxidative, anti-inflammatory, anti-diabetic activity and anti-obesity. It suggests that Lythrum salicaria L. could be potentially used as a resource of bioactive materials for health functional foods.

The objectives of this study were to compare the antioxidant activities by high pressure extraction of Codonopsis lanceolata from different cultivation areas; Hoeng-sung, Jeju island, and China. Total phenolic acid contents of Hoeng-sung, Jeju, China were estimated as 732.11, 640.25, and 584.85 mg QUR/100 g DW, respectively. The flavonoids contents of Hoeng-sung, Jeju, China were measured as 80.37, 76.46, and 74.55 mg QUR/100g DW, respectively. Generally, contents of phenolic acid and flavonoids, HPE was higher than conventional extraction process. Hoeng-sung Codonopsis lanceolata showed 64.33% of DPPH radical scavenging activity (EDA, %) in 3.2 mg/ml of Hoeng-sung Codonopsis lanceolata. The reducing power of Hoeng-sung cultivation area Codonopsis lanceolata also showed the high activity as 3.15. In generally, antioxidant activities of Codonopsis lanceolata were increased by high pressure extraction process. Based on these results, higher contents of flavonoids and total polyphenols were found extracted by high pressure extraction of Codonopsis lanceolata grow in Hoeng-sung area than others.