본 연구는 다양한 결실기로 육성한 산초나무 5개의 신품종(한초 915호, 한초 930호, 한초 10호, 한초 1020호, 한초 1030호)에 대해 잎과 가시의 형태적 특성을 조사하여 비교하였다. 조사는 질적 특성과 양적 특성으로 구분하여 실시하였으며, 양적 특성은 일원배치 분산분석(One-Way ANOVA)과 Scheffe의 다중검증(Scheffe's multiple range test)으로 품종 간 차이를 확인하였다. 또한 요인분석을 이용하여 조사한 특성의 기여도를 확인하였다. 그 결과 잎 길이에서는 유의한 차이를 확인하지 못했으나, 정소엽의 거치 수, 소엽의 수, 가시의 크기에서 품종 간 유의한 차이가 있었으며, 요인분석도 유사한 결과를 확인 하였다. 또한 비가중평균결합(Unweighted pair-group method using arithmetic averages) 군집분석을 수지도(dendrogram)로 확인한 결과, 한초 1020호와 1030호가 근접하고, 한초 915호와 930호가 근접하였으나 개화기와 결실기에서 뚜렷한 차이로 품종이 구분되는 것을 확인할 수 있었다.

Elite trees of evergreen oaks (Quercus glauca, Quercus acuta, Quercus salicina, and Quercus gilva) were selected from Jeju Island and Wando Island. Elite trees were carried out by modifying the tree selection criteria. Elite trees were selected by height, DBH, clear length, crown diameter, leaf length, and leaf depth. Regarding height, Q. acuta was the highest, and the other three tree species were similar. Clear length showed the same trend as height. In the case of Q. glauca, height showed a positive correlation with DBH, crown diameter and leaf depth. In the case of Q. acuta, positive correlations were shown with all characteristics of DBH, and correlation analysis between DBH and crown diameter, and leaf length and leaf depth also showed positive correlations. In the Pearson correlation coefficient of Q. salicina, height showed a positive correlation with DBH. In the case of Q. gilva, height showed a positive correlation with DBH (0.539). As a result of analyzing the principal components for each of the six growth characteristics, the four species were divided into two principal components with an eigenvalue of 1 or higher, and the cumulative explanatory power was 57% or more. Based on the principal component results, it was possible to confirm the relationship between growth and trait characteristics by species. Still, it was not easy to understand the relationship among each selection tree, so a cluster analysis was performed using the principal component score. Based on the distance levels 5.0 and 6.0 of the selection tree of each species, they were classified into 4-5 clusters. It is judged that the above results can be used as data for the selection of elite trees of evergreen oaks.

In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines

딸기 묘의 크라운 크기가 정식 후 식물체 생육 및 과실의 수량에 미치는 영향에 관하여 알아보고자 하였다. 묘 크기의 기준은 크라운 직경의 크기로 하여 3등급으로 구분하였으며, A등급 묘는 9.0 mm이상, B등급 묘 8.0∼8.9 mm, C등급 묘 8.0 mm이하로 하였다. 정식기 묘의 크라운 크기가 클수록 정식 후 뿌리 활착 및 식물체 생육이 좋았고, 개화기도 빨랐다. 정화방 첫 꽃 개화기는 A등급 묘가 B등급, C등급 묘에 비해 10일, 평균 개화기는 2∼3일 정도 빨랐다. A등급 묘의 1주당 4월까지 누적수량은 481 g, B등급 445 g, C등급 422 g 으로서, 크라운 직경이 가장 큰 A등급 묘는 B등급, C등급 묘에 비해 각각 8.1%, 13.9% 증수되었고, 2월까지의 초기수량은 A등급 묘가 B, C등급 묘에 비해 각각 23.3%, 39.1% 많았다.

An efficient method for the rapid micropropagation of Camptotheca acuminata from axillary buds was established by application of various plant growth regulators. Among various cytokinins, 0.5 mg L-1 BA showed the best performance on shoot multiplication, number average multiple shoots up to 10.8. The propagated shoot cuttings in vitro were elongated on NN basal medium without plant growth regulators. The secondary multiple shoots were induced at the site of initially induced buds. Rooting was induced directly near the base of the shoot on half-strength NN medium containing 0.5 mg L-1 of IBA, whereas high concentration of 1.0 mg L-1 IBA could induce callus at the base of the shoot. The camptothecin content, anticancer compound of the micropropagated plants was contained in various tissues. Camptothecin contents were 1.8 and 2.5 mg g-1 dry weight in stems from propagated in vitro and mother plant, respectively. This result may be used to develop strategies for large-scale propagation of elite C. acuminata trees.

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, 0.5 mg L -1 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins α-napthalene acetic acid (NAA), indole acetic acid (IAA) and β-indolebutyric acid (IBA) (0.5 and 1.0 mg L -1 ). The 1.0 mg L -1 IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.