This study analyzed the patterns of relative clauses (RCs) used on the College Scholastic Ability Test (CSAT), a standardized test for college admissions in Korea. The researchers investigated the Noun Phrase Accessibility Hierarchy and the impact of the antecedent’s animacy on RCs. Also, it was explored how NPs in the subject position of object RCs affected language processing, specifically, the condition where relativizers were omitted and the reasons for producing passive subject RCs. Data were collected from the CSAT and mock tests from 2019 to 2023. The analysis of 622 RCs yielded the following results: First, the frequency of RCs appeared in the following order: subject RCs, object RCs, then OBL. Second, the object RCs showed a preference for inanimate antecedents whereas the subject RCs used did not show a preference for animate antecedents. Third, NPs in the subject position with sentences containing object RCs predominantly took the form of pronouns, with common nouns used less frequently.

We developed a Amylose magnetic beads (AMBs) based detection system for high efficient separation, concentration and detection of E. coli O157:H7 in real sample. AMBs were synthesized by amylosucrase from Deinococcus geothermalis (DgAS) with iron-oxide nanoparticle (NP). The design of amylose magnetic beads (AMBs) have studied by an enzymatic synthesis with optimized reaction condition such as substrate, sucrose, and iron-oxide NP. AMBs have specific feature. AMBs decorated with functional fusion protein, which consists in a maltose binding protein (MBP) and a streptococcal protein G (SPG). Amylose chains has maltose, thus MBP-SPG binds to the AMB. In addition, SPG specifically binds to the Fc part of antibody. That was used as a linker to immobilize antibody to the surface of AMBs. The resulting AMBs were efficiently separated and concentrated target bacteria, E. coli O157:H7. Concentrated sample is qualitatively analyzed by PCR. Our studies demonstrated that AMB-based PCR significantly reduced the limitation of detection as low as 10 1 CFU/mL, compared to that of conventional PCR. The principle of this system can be served as a high efficiency for detection method of any pathogenic bacteria. In addition, AMBs and MBP-SPG cross-linker protein developed in this study is expected to be applicable to the portable food based biological processing monitoring system.